中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
8期
1816-1818
,共3页
黄林飞%卢玉娟%潘晟%罗浩
黃林飛%盧玉娟%潘晟%囉浩
황림비%로옥연%반성%라호
胃癌%血管内皮生长因子%基质金属蛋白酶-2%侵袭
胃癌%血管內皮生長因子%基質金屬蛋白酶-2%侵襲
위암%혈관내피생장인자%기질금속단백매-2%침습
Gastric cancer%Vascular endothelial growth factor%Matrix metalloproteinase-2%Invasion
目的 观察胃癌组织中血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)的表达,探讨两者与胃癌侵袭、转移的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法检测30例胃癌癌前病变及42例胃癌组织中VEGF、MMP-2的表达.结果 VEGF在浅表性胃炎、慢性萎缩性胃炎伴肠化、不典型增生、早期胃癌和进展期胃癌中VEGF阳性表达率分别为25.0％ (3/12)、36.3％ (4/11)、42.8％(3/7)、57.1％ (8/14)、78.5％ (22/28);MMP-2在浅表性胃炎、慢性萎缩性胃炎伴肠化、不典型增生、早期胃癌和进展期胃癌中MMP-2阳性表达率分别16.7％(2/12)、27.2％ (3/11)、28.6％(2/7)、42.8％ (6/14)、76.2％(20/28).VEGF、MMP-2阳性表达率随着胃癌的发展逐渐升高.42例胃癌组织中,VEGF、MMP-2阳性率在侵及浆膜层组为73.1％和80.8％,显著高于未侵及浆膜层组(56.3％和62.5％,P＜ 0.05);在淋巴结转移组为79.2％和87.5％,显著高于无淋巴结转移组(33.3％和44.4％,P＜0.05);胃癌组织中VEGF、MMP-2表达呈正相关(r =0.416,P＜0.05).结论 VEGF、MMP-2高表达与胃癌侵袭密切相关.
目的 觀察胃癌組織中血管內皮生長因子(VEGF)、基質金屬蛋白酶-2(MMP-2)的錶達,探討兩者與胃癌侵襲、轉移的關繫.方法 採用免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)法檢測30例胃癌癌前病變及42例胃癌組織中VEGF、MMP-2的錶達.結果 VEGF在淺錶性胃炎、慢性萎縮性胃炎伴腸化、不典型增生、早期胃癌和進展期胃癌中VEGF暘性錶達率分彆為25.0％ (3/12)、36.3％ (4/11)、42.8％(3/7)、57.1％ (8/14)、78.5％ (22/28);MMP-2在淺錶性胃炎、慢性萎縮性胃炎伴腸化、不典型增生、早期胃癌和進展期胃癌中MMP-2暘性錶達率分彆16.7％(2/12)、27.2％ (3/11)、28.6％(2/7)、42.8％ (6/14)、76.2％(20/28).VEGF、MMP-2暘性錶達率隨著胃癌的髮展逐漸升高.42例胃癌組織中,VEGF、MMP-2暘性率在侵及漿膜層組為73.1％和80.8％,顯著高于未侵及漿膜層組(56.3％和62.5％,P＜ 0.05);在淋巴結轉移組為79.2％和87.5％,顯著高于無淋巴結轉移組(33.3％和44.4％,P＜0.05);胃癌組織中VEGF、MMP-2錶達呈正相關(r =0.416,P＜0.05).結論 VEGF、MMP-2高錶達與胃癌侵襲密切相關.
목적 관찰위암조직중혈관내피생장인자(VEGF)、기질금속단백매-2(MMP-2)적표체,탐토량자여위암침습、전이적관계.방법 채용면역조직화학련매균항생물소단백-과양화물매(SP)법검측30례위암암전병변급42례위암조직중VEGF、MMP-2적표체.결과 VEGF재천표성위염、만성위축성위염반장화、불전형증생、조기위암화진전기위암중VEGF양성표체솔분별위25.0％ (3/12)、36.3％ (4/11)、42.8％(3/7)、57.1％ (8/14)、78.5％ (22/28);MMP-2재천표성위염、만성위축성위염반장화、불전형증생、조기위암화진전기위암중MMP-2양성표체솔분별16.7％(2/12)、27.2％ (3/11)、28.6％(2/7)、42.8％ (6/14)、76.2％(20/28).VEGF、MMP-2양성표체솔수착위암적발전축점승고.42례위암조직중,VEGF、MMP-2양성솔재침급장막층조위73.1％화80.8％,현저고우미침급장막층조(56.3％화62.5％,P＜ 0.05);재림파결전이조위79.2％화87.5％,현저고우무림파결전이조(33.3％화44.4％,P＜0.05);위암조직중VEGF、MMP-2표체정정상관(r =0.416,P＜0.05).결론 VEGF、MMP-2고표체여위암침습밀절상관.
Objective To investigate the relationship between expression levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 and gastric cane invasion.Methods Streptavid-in-peroxidase (SP) immunohistochemical method was used to detect the expression of VEGF,MMP-2 in 30 cases of precancerous lesions and 42 cases of gastric carcinoma.Results The positive rate of VEGF in superficial gastritis,chronic atrophic gastritis with intestinal metaplasia,atypical hyperplasia,early gastric cancer,progressive stomach cancer were 25.0％ (3/12),36.3％ (4/11),42.8％ (3/7),57.1％ (8/14),78.5％ (22/28).The positive rate of MMP-2 in superficial gastritis,chronic atrophic gastritis with intestinal metaplasia,atypical hyperplasia,early gastric cancer,progressive stomach cancer were 16.7％ (2/12),27.2％ (3/11),28.6％ (2/7),42.8％ (6/14),76.2％ (20/28).With development of gastric carcinoma,the positive rate of VEGF,MMP-2 were increased gradually (P ＜ 0.05).The positive rate of VEGF,MMP-2 in group of seroasinfiltration (73.1％,80.8％)were significantly high-er than that in group of outseroasinfiltration (56.3％,62.5 ％) (P ＜ 0.05).The positive rate of VEGF,MMP-2 in group of lymph node metastasis (79.2％,87.5％)were higher than that in group without lymph node metastasis (33.3％,44.4％) (P ＜ 0.05).There was a relationship between VEGF and MMP-2 expression (P ＜ 0.05).Conclusion Both VEGF and MMP-2 contribute to the development,invasion and metastasis of gastric carcinoma.
