CAJ | 학술논문

目的检测音猬因子(Shh)、Smoothened (Smo)、胶质母细胞瘤转录因子(Gli1)在胰腺癌组织和癌旁组织中表达的差异,探讨Shh、Smo、Gli1在胰腺癌中异常表达与肿瘤发生的关系.方法选取50例手术切除的新鲜胰腺癌组织和癌旁组织,应用逆转录聚合酶链反应(RT-PCR)技术和Western blot检测胰腺癌组织和癌旁组织中Shh、Smo、Gli1 mRNA和蛋白的表达,并分析其与胰腺癌临床病理特征之间的关系.结果 RT-PCR检测结果显示:Shh、Smo、Gli1 mRNA在胰腺癌组织中的相对表达量分别为0.309 ±0.162、0.327±0.264、0.341 ±0.132,在胰腺癌旁组织中为0.184±0.227、0.148 ±0.276、0.105±0.351(P＜0.05).Western blot检测结果显示:Shh、Smo、Gli1蛋白在胰腺癌组织中的相对表达量分别为0.624 ±0.139、0.547 ±0.418、0.563 ±0.271,在胰腺癌旁组织中为0.388 ±0.294、0.294 ±0.152、0.328 ±0.129(P＜0.05).胰腺癌组织中Shh、Smo、Gli1 mRNA与蛋白表达均高于癌旁组织.胰腺癌组织中Shh、Smo、Gli1 mRNA与蛋白表达均与胰腺癌的分化程度相关(P＜0.05),与患者年龄、肿瘤直径、TNM分期、侵犯血管和神经、淋巴结转移、远处转移无相关(P＞0.05).结论 Shh、Smo、Gli1信号分子在胰腺癌组织中表达增高,Hedgehog信号途径可能在胰腺癌发生发展过程中起重要作用.
목적 검측음위인자(Shh)、Smoothened (Smo)、효질모세포류전록인자(Gli1)재이선암조직화암방조직중표체적차이,탐토Shh、Smo、Gli1재이선암중이상표체여종류발생적관계.방법 선취50례수술절제적신선이선암조직화암방조직,응용역전록취합매련반응(RT-PCR)기술화Western blot검측이선암조직화암방조직중Shh、Smo、Gli1 mRNA화단백적표체,병분석기여이선암림상병리특정지간적관계.결과 RT-PCR검측결과현시:Shh、Smo、Gli1 mRNA재이선암조직중적상대표체량분별위0.309 ±0.162、0.327±0.264、0.341 ±0.132,재이선암방조직중위0.184±0.227、0.148 ±0.276、0.105±0.351(P＜0.05).Western blot검측결과현시:Shh、Smo、Gli1단백재이선암조직중적상대표체량분별위0.624 ±0.139、0.547 ±0.418、0.563 ±0.271,재이선암방조직중위0.388 ±0.294、0.294 ±0.152、0.328 ±0.129(P＜0.05).이선암조직중Shh、Smo、Gli1 mRNA여단백표체균고우암방조직.이선암조직중Shh、Smo、Gli1 mRNA여단백표체균여이선암적분화정도상관(P＜0.05),여환자년령、종류직경、TNM분기、침범혈관화신경、림파결전이、원처전이무상관(P＞0.05).결론 Shh、Smo、Gli1신호분자재이선암조직중표체증고,Hedgehog신호도경가능재이선암발생발전과정중기중요작용.
Objective To investigate the differential expression of sonic hedgehog (Shh),Smoothened (Smo) and Glioblastoma transcriptional factor (Gli1) in the pancreatic cancers and their adjacent tissues,and their correlation with pathogenesis of pancreatic cancer.Methods Reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blotting were used to determine the mRNA and protein expression of Shh,Smo and Gli1 in 50 cases of pancreatic cancers and their adjacent tissues.The relationship between the expression of Shh,Smo and Gli1 and the clinicopathological features of pancreatic cancer was analyzed.Results The expression of Shh,Smo and Gli1 mRNA in pancreatic cancer was 0.309 ± 0.162,0.327 ±0.264 and 0.341 ±0.132,and that in the para-cancerous adjacent tissue was 0.184 ± 0.227,0.148 ±0.276 and 0.105 ±0.351 respectively (P ＜0.05).The expression of Shh,Smo and Gli1 protein in pancreatic cancer was 0.624 ±0.139,0.547 ±0.418 and 0.563 ±0.271,and that in paracancerous adjacent tissue was 0.388 ± 0.294,0.294 ± 0.152 and 0.328 ± 0.129 respectively (P ＜ 0.05).The expression levels of Shh,Smo and Gli1 mRNA and protein in pancreatic cancer tissue were higher than in paracancerous adjacent tissue.The overexpression of Shh,Smo and Gli1 was correlated with differentitation of pancreatic cancer cells (P ＜ 0.05),but not with age,tumor size,TNM grade,invasion to nerves and vascellum,lymph node metastasis and distant metastasis (P ＞ 0.05).Conclusion The expression level of Shh,Smo and Gli1 is increased in human pancreatic cancer cells.The overexpression of hedgehog signaling pathway might play an important role in carcinogenesis and development of pancreatic cancer.