CAJ | 학술논문

目的观察神经酰胺信号通路在花生四烯酸乙醇胺(AEA)抑制人胶质瘤U251细胞黏附和侵袭生物学行为的抑制作用.方法通过5 μmol/L AEA、5μmol/L AEA+ 10 μmol/L C2-神经酰胺作用24h的预处理U251细胞,同时与先用10 μmol/L烟曲霉毒素(FB1)预处理U251细胞24 h后的实验组比较,应用噻唑蓝(MTT)比色法测定U251细胞黏附作用的影响,应用Transwell小室实验检测U251细胞侵袭能力的影响.结果 5μmol/L AEA、5μmol/L AEA+ 10 μmol/L C2-神经酰胺均能抑制U251细胞侵袭生物学行为的作用,5μmol/L AEA组U251细胞黏附和侵袭能力分别降低66.53％和83.85％,5μmol/L AEA+ 10 μmol/L C2-神经酰胺组分别降低71.64％和86.43％;而用10 μmol/L FB1先预处理U251细胞24 h后,5μmol/L AEA组U251细胞黏附和侵袭能力仅分别降低27.43％和32.23％,5μmol/L AEA+ 10 μmol/L C2-神经酰胺组则分别降低56.61％和68.11％.结论 AEA具有人胶质瘤U251细胞黏附和侵袭生物学行为的抑制作用,这种作用可能是通过慢反应神经酰胺信号通路来实现的.
목적 관찰신경선알신호통로재화생사희산을순알(AEA)억제인효질류U251세포점부화침습생물학행위적억제작용.방법 통과5 μmol/L AEA、5μmol/L AEA+ 10 μmol/L C2-신경선알작용24h적예처리U251세포,동시여선용10 μmol/L연곡매독소(FB1)예처리U251세포24 h후적실험조비교,응용새서람(MTT)비색법측정U251세포점부작용적영향,응용Transwell소실실험검측U251세포침습능력적영향.결과 5μmol/L AEA、5μmol/L AEA+ 10 μmol/L C2-신경선알균능억제U251세포침습생물학행위적작용,5μmol/L AEA조U251세포점부화침습능력분별강저66.53％화83.85％,5μmol/L AEA+ 10 μmol/L C2-신경선알조분별강저71.64％화86.43％;이용10 μmol/L FB1선예처리U251세포24 h후,5μmol/L AEA조U251세포점부화침습능력부분별강저27.43％화32.23％,5μmol/L AEA+ 10 μmol/L C2-신경선알조칙분별강저56.61％화68.11％.결론 AEA구유인효질류U251세포점부화침습생물학행위적억제작용,저충작용가능시통과만반응신경선알신호통로래실현적.
Objective To observe the effect of ceramide signaling pathway on anandamide (AEA)-mediated inhibition of adhesion and invasion of U251 glioma cell line.Methods Human U251 cells were cultured with DMEM in vitro.Cultured cells were divided into control group,5 μmol/L AEA group,and 5 μmol/L AEA + 10 μmol/L C2-ceramide group.At the same time,the AEA groups were pretreated with 10 μmol/L fumonisin B1 (FB1) for 24 h early.The cell adhesion rate of U251 cells was examined by methyl thiazol tetrazolium (MTT) assay at different time points of AEA treatment.The abilities of invasion were detected by Transwell assay.Results 5 μmol/L AEA,and 5 μmol/L AEA + 10 μmol/L C2-ceramide could inhibit invasive abilities of U251 cells.The capabilities of adhesion and invasion of U251 cells in 5 μmol/L AEA group was reduced by 66.53％ and 83.85％ respectively (P ＜ 0.01),and those in 5 μmol/L AEA + 10 μmol/L C2-ceramide group were decreased by 71.64％ and 86.43％ respectively (P ＜0.01).After pretreatment of U251 cells with 10 μmol/L FB1 for 24 h,The capabilities of adhesion and invasion of U251 cells in 5 μ mol/L AEA group were only decreased by 27.43％ and 32.23％,and those in 5 μmol/L AEA + 10 μmol/L C2-ceramide group decreased by 56.61％ and 68.11％ (P ＜0.01),respectively.Conclusion AEA can inhibit the capabilities of adhesion and invasion of U251 cells via the ceramide signaling de novo synthesis pathway.