中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1877-1879,后插1
,共4页
扈玉华%吴建梁%刘兵%田红伟
扈玉華%吳建樑%劉兵%田紅偉
호옥화%오건량%류병%전홍위
胶质瘤%N-乙酰氨基半乳糖转移酶-14%脱噬作用%O-糖基化
膠質瘤%N-乙酰氨基半乳糖轉移酶-14%脫噬作用%O-糖基化
효질류%N-을선안기반유당전이매-14%탈서작용%O-당기화
Glioma%N-acetyl amino galactosyl transferase-14%Apoptosis%O-glycosylation
目的 探讨N-乙酰氨基半乳糖转移酶-14 (GalNAc-T14)在不同级别恶性胶质瘤中的表达差异及其与胶质瘤恶性程度的关系.方法 采用实时荧光定量核酸扩增检测系统(QPCR)方法和免疫组织化学染色方法检测16例正常脑组织标本、11例Ⅱ级脑胶质瘤组织标本、14例Ⅲ级脑胶质瘤组织标本和9例Ⅳ级脑胶质瘤组织标本中GalNAc-T14的表达水平.结果 QPCR方法显示GalNAc-T14在正常脑组织标本及Ⅱ、Ⅲ、Ⅳ级脑胶质瘤细胞标本中的电泳条带的吸光度值分别为0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.单因素方差分析及LSD、SNK-t分析比较,GalNAc-T14在胶质瘤细胞组中的表达与正常脑组织组差异有统计学意义(P<0.05);GalNAc-T14在Ⅱ、Ⅲ、Ⅳ级星型胶质瘤中的表达差异有统计学意义(P<0.05).免疫组织化学染色方法结果显示GalNAc-T14在正常脑组织标本中和Ⅱ、Ⅲ、Ⅳ级脑胶质瘤细胞标本中的阳性表达率分别为68.7%、63.6%、42.9%和33.3%,x2检验及Fisher确切概率法分析比较,GalNAc-T14在胶质瘤细胞组中的表达与正常脑组织组比较差异有统计学意义(P<0.05);不同级别的脑胶质瘤细胞中,表达存在一定差异.结论 GalNAc-T14在恶性胶质瘤细胞中的表达水平较正常脑组织中明显降低,与病情进展密切相关.
目的 探討N-乙酰氨基半乳糖轉移酶-14 (GalNAc-T14)在不同級彆噁性膠質瘤中的錶達差異及其與膠質瘤噁性程度的關繫.方法 採用實時熒光定量覈痠擴增檢測繫統(QPCR)方法和免疫組織化學染色方法檢測16例正常腦組織標本、11例Ⅱ級腦膠質瘤組織標本、14例Ⅲ級腦膠質瘤組織標本和9例Ⅳ級腦膠質瘤組織標本中GalNAc-T14的錶達水平.結果 QPCR方法顯示GalNAc-T14在正常腦組織標本及Ⅱ、Ⅲ、Ⅳ級腦膠質瘤細胞標本中的電泳條帶的吸光度值分彆為0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.單因素方差分析及LSD、SNK-t分析比較,GalNAc-T14在膠質瘤細胞組中的錶達與正常腦組織組差異有統計學意義(P<0.05);GalNAc-T14在Ⅱ、Ⅲ、Ⅳ級星型膠質瘤中的錶達差異有統計學意義(P<0.05).免疫組織化學染色方法結果顯示GalNAc-T14在正常腦組織標本中和Ⅱ、Ⅲ、Ⅳ級腦膠質瘤細胞標本中的暘性錶達率分彆為68.7%、63.6%、42.9%和33.3%,x2檢驗及Fisher確切概率法分析比較,GalNAc-T14在膠質瘤細胞組中的錶達與正常腦組織組比較差異有統計學意義(P<0.05);不同級彆的腦膠質瘤細胞中,錶達存在一定差異.結論 GalNAc-T14在噁性膠質瘤細胞中的錶達水平較正常腦組織中明顯降低,與病情進展密切相關.
목적 탐토N-을선안기반유당전이매-14 (GalNAc-T14)재불동급별악성효질류중적표체차이급기여효질류악성정도적관계.방법 채용실시형광정량핵산확증검측계통(QPCR)방법화면역조직화학염색방법검측16례정상뇌조직표본、11례Ⅱ급뇌효질류조직표본、14례Ⅲ급뇌효질류조직표본화9례Ⅳ급뇌효질류조직표본중GalNAc-T14적표체수평.결과 QPCR방법현시GalNAc-T14재정상뇌조직표본급Ⅱ、Ⅲ、Ⅳ급뇌효질류세포표본중적전영조대적흡광도치분별위0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.단인소방차분석급LSD、SNK-t분석비교,GalNAc-T14재효질류세포조중적표체여정상뇌조직조차이유통계학의의(P<0.05);GalNAc-T14재Ⅱ、Ⅲ、Ⅳ급성형효질류중적표체차이유통계학의의(P<0.05).면역조직화학염색방법결과현시GalNAc-T14재정상뇌조직표본중화Ⅱ、Ⅲ、Ⅳ급뇌효질류세포표본중적양성표체솔분별위68.7%、63.6%、42.9%화33.3%,x2검험급Fisher학절개솔법분석비교,GalNAc-T14재효질류세포조중적표체여정상뇌조직조비교차이유통계학의의(P<0.05);불동급별적뇌효질류세포중,표체존재일정차이.결론 GalNAc-T14재악성효질류세포중적표체수평교정상뇌조직중명현강저,여병정진전밀절상관.
Objective To explore the expression differences of N-acetyl amino galactosyl transferase-14 (GalNAc-T14) in normal brain tissue and different levels of malignant gliomas and to observe the relationship between the expression of GalNAc-T14 with the differentiation and malignant degree of gliomas.Methods By real-time quantitative polymerase chain reaction detecting system (QPCR) and immunohistochemical staining methods,the expression of GalNAc-T14 was detected in 11 cases of Ⅱ grade glioma tissue samples,14 cases of Ⅲ grade glioma tissue specimens,9 cases of Ⅳ grade glioma tissue samples and 16 specimens of normal brain tissue.Results The average optical density values of GalNAc-T14 in normal brain tissue specimens and the specimens of Ⅱ,Ⅲ and Ⅳ grade glioma cells were 0.956 4 ±0.079 1,0.525 6 ±0.064 7,0.298 9 ±0.080 8,and0.151 4 ±0.062 4 respectively by QPCR.Single factor analysis of variance (One-way ANOVA) and LSD,SNK-t analysis comparison showed the expression of GalNAc-T14 in human gliomas was significantly different from that in the normal brain tissue (P < 0.05).The same significance was also found between grade Ⅱ and grade Ⅲ or grade Ⅳ (P < 0.05).Immunohistochemical staining method revealed that the positive rate of the GalNAc-T14 expression was 68.7% in normal brain tissue specimens and 63.6%,42.9% and 33.3% in glioma tissues of grade Ⅱ,Ⅲ and Ⅳ,and there was significant difference between glioma group and normal brain tissue groups (P < 0.05).Different levels of brain glioma cells showed no obvious consistency.Conclusion The expression of GalNAc-T14 was significantly reduced in malignant glioma cells,and was closely related with disease progression.