中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1877-1879,后插1
,共4页
扈玉华%吴建梁%刘兵%田红伟
扈玉華%吳建樑%劉兵%田紅偉
호옥화%오건량%류병%전홍위
胶质瘤%N-乙酰氨基半乳糖转移酶-14%脱噬作用%O-糖基化
膠質瘤%N-乙酰氨基半乳糖轉移酶-14%脫噬作用%O-糖基化
효질류%N-을선안기반유당전이매-14%탈서작용%O-당기화
Glioma%N-acetyl amino galactosyl transferase-14%Apoptosis%O-glycosylation
目的 探讨N-乙酰氨基半乳糖转移酶-14 (GalNAc-T14)在不同级别恶性胶质瘤中的表达差异及其与胶质瘤恶性程度的关系.方法 采用实时荧光定量核酸扩增检测系统(QPCR)方法和免疫组织化学染色方法检测16例正常脑组织标本、11例Ⅱ级脑胶质瘤组织标本、14例Ⅲ级脑胶质瘤组织标本和9例Ⅳ级脑胶质瘤组织标本中GalNAc-T14的表达水平.结果 QPCR方法显示GalNAc-T14在正常脑组织标本及Ⅱ、Ⅲ、Ⅳ级脑胶质瘤细胞标本中的电泳条带的吸光度值分别为0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.单因素方差分析及LSD、SNK-t分析比较,GalNAc-T14在胶质瘤细胞组中的表达与正常脑组织组差异有统计学意义(P＜0.05);GalNAc-T14在Ⅱ、Ⅲ、Ⅳ级星型胶质瘤中的表达差异有统计学意义(P＜0.05).免疫组织化学染色方法结果显示GalNAc-T14在正常脑组织标本中和Ⅱ、Ⅲ、Ⅳ级脑胶质瘤细胞标本中的阳性表达率分别为68.7％、63.6％、42.9％和33.3％,x2检验及Fisher确切概率法分析比较,GalNAc-T14在胶质瘤细胞组中的表达与正常脑组织组比较差异有统计学意义(P＜0.05);不同级别的脑胶质瘤细胞中,表达存在一定差异.结论 GalNAc-T14在恶性胶质瘤细胞中的表达水平较正常脑组织中明显降低,与病情进展密切相关.
目的 探討N-乙酰氨基半乳糖轉移酶-14 (GalNAc-T14)在不同級彆噁性膠質瘤中的錶達差異及其與膠質瘤噁性程度的關繫.方法 採用實時熒光定量覈痠擴增檢測繫統(QPCR)方法和免疫組織化學染色方法檢測16例正常腦組織標本、11例Ⅱ級腦膠質瘤組織標本、14例Ⅲ級腦膠質瘤組織標本和9例Ⅳ級腦膠質瘤組織標本中GalNAc-T14的錶達水平.結果 QPCR方法顯示GalNAc-T14在正常腦組織標本及Ⅱ、Ⅲ、Ⅳ級腦膠質瘤細胞標本中的電泳條帶的吸光度值分彆為0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.單因素方差分析及LSD、SNK-t分析比較,GalNAc-T14在膠質瘤細胞組中的錶達與正常腦組織組差異有統計學意義(P＜0.05);GalNAc-T14在Ⅱ、Ⅲ、Ⅳ級星型膠質瘤中的錶達差異有統計學意義(P＜0.05).免疫組織化學染色方法結果顯示GalNAc-T14在正常腦組織標本中和Ⅱ、Ⅲ、Ⅳ級腦膠質瘤細胞標本中的暘性錶達率分彆為68.7％、63.6％、42.9％和33.3％,x2檢驗及Fisher確切概率法分析比較,GalNAc-T14在膠質瘤細胞組中的錶達與正常腦組織組比較差異有統計學意義(P＜0.05);不同級彆的腦膠質瘤細胞中,錶達存在一定差異.結論 GalNAc-T14在噁性膠質瘤細胞中的錶達水平較正常腦組織中明顯降低,與病情進展密切相關.
목적 탐토N-을선안기반유당전이매-14 (GalNAc-T14)재불동급별악성효질류중적표체차이급기여효질류악성정도적관계.방법 채용실시형광정량핵산확증검측계통(QPCR)방법화면역조직화학염색방법검측16례정상뇌조직표본、11례Ⅱ급뇌효질류조직표본、14례Ⅲ급뇌효질류조직표본화9례Ⅳ급뇌효질류조직표본중GalNAc-T14적표체수평.결과 QPCR방법현시GalNAc-T14재정상뇌조직표본급Ⅱ、Ⅲ、Ⅳ급뇌효질류세포표본중적전영조대적흡광도치분별위0.956 4±0.079 1、0.525 6±0.0647、0.298 9±0.080 8、0.151 4±0.0624.단인소방차분석급LSD、SNK-t분석비교,GalNAc-T14재효질류세포조중적표체여정상뇌조직조차이유통계학의의(P＜0.05);GalNAc-T14재Ⅱ、Ⅲ、Ⅳ급성형효질류중적표체차이유통계학의의(P＜0.05).면역조직화학염색방법결과현시GalNAc-T14재정상뇌조직표본중화Ⅱ、Ⅲ、Ⅳ급뇌효질류세포표본중적양성표체솔분별위68.7％、63.6％、42.9％화33.3％,x2검험급Fisher학절개솔법분석비교,GalNAc-T14재효질류세포조중적표체여정상뇌조직조비교차이유통계학의의(P＜0.05);불동급별적뇌효질류세포중,표체존재일정차이.결론 GalNAc-T14재악성효질류세포중적표체수평교정상뇌조직중명현강저,여병정진전밀절상관.
Objective To explore the expression differences of N-acetyl amino galactosyl transferase-14 (GalNAc-T14) in normal brain tissue and different levels of malignant gliomas and to observe the relationship between the expression of GalNAc-T14 with the differentiation and malignant degree of gliomas.Methods By real-time quantitative polymerase chain reaction detecting system (QPCR) and immunohistochemical staining methods,the expression of GalNAc-T14 was detected in 11 cases of Ⅱ grade glioma tissue samples,14 cases of Ⅲ grade glioma tissue specimens,9 cases of Ⅳ grade glioma tissue samples and 16 specimens of normal brain tissue.Results The average optical density values of GalNAc-T14 in normal brain tissue specimens and the specimens of Ⅱ,Ⅲ and Ⅳ grade glioma cells were 0.956 4 ±0.079 1,0.525 6 ±0.064 7,0.298 9 ±0.080 8,and0.151 4 ±0.062 4 respectively by QPCR.Single factor analysis of variance (One-way ANOVA) and LSD,SNK-t analysis comparison showed the expression of GalNAc-T14 in human gliomas was significantly different from that in the normal brain tissue (P ＜ 0.05).The same significance was also found between grade Ⅱ and grade Ⅲ or grade Ⅳ (P ＜ 0.05).Immunohistochemical staining method revealed that the positive rate of the GalNAc-T14 expression was 68.7％ in normal brain tissue specimens and 63.6％,42.9％ and 33.3％ in glioma tissues of grade Ⅱ,Ⅲ and Ⅳ,and there was significant difference between glioma group and normal brain tissue groups (P ＜ 0.05).Different levels of brain glioma cells showed no obvious consistency.Conclusion The expression of GalNAc-T14 was significantly reduced in malignant glioma cells,and was closely related with disease progression.
