中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1892-1894,后插1-后插2
,共4页
神经胶质瘤%肿瘤干细胞%免疫组织化学%疱疹病毒相关性泛素特异性蛋白酶%CD133
神經膠質瘤%腫瘤榦細胞%免疫組織化學%皰疹病毒相關性汎素特異性蛋白酶%CD133
신경효질류%종류간세포%면역조직화학%포진병독상관성범소특이성단백매%CD133
Glioma%Cancer stem cell%Immunohistochemistry%Herpesvirus-associated ubiquitin-specific protease%CD133
目的 研究人脑胶质瘤中疱疹病毒相关性泛素特异性蛋白酶(HAUSP)与脑肿瘤干细胞(BTSCs)标志物CD133之间表达的关系,并探讨其在脑胶质瘤发生发展中的意义.方法 (1)取人脑胶质瘤组织标本72例,采用免疫组织化学染色检测HAUSP、CD133的表达,比较不同级别胶质瘤HAUSP+细胞和CD133+细胞所占百分比并分析其与胶质瘤级别的相关性.(2)血清剥夺法从U87胶质瘤细胞株分离培养BTSCs,通过全反式维甲酸(ATRA)诱导分化,免疫细胞荧光双重染色观察HAUSP及CD133在不同时期表达.结果 Ⅱ～Ⅳ级胶质瘤中HAUSP+细胞率分别为(25.9±11.3)％、(49.8±19.8)％、(63.7±20.4)％;CD133+细胞率分别为(12.60±3.49)％、(18.90±5.81)％、(24.80±7.54)％,不同级别胶质瘤中HAUSP+细胞、CD133+细胞百分比差异均有统计学意义(P＜0.05),高级别组胶质瘤中HAUSP+细胞、CD133+细胞表达水平高于低级别组,差异有统计学意义(P＜0.05);HAUSP+表达和CD133+表达呈正相关(r=0.915,P＜0.01);随着U87胶质瘤干细胞分化,HAUSP、CD133表达逐渐减少.结论 HAUSP与干细胞标志物CD133之间有明显的相关性.HAUSP在维持脑胶质瘤干细胞未分化或低分化的状态中可能起到重要作用.
目的 研究人腦膠質瘤中皰疹病毒相關性汎素特異性蛋白酶(HAUSP)與腦腫瘤榦細胞(BTSCs)標誌物CD133之間錶達的關繫,併探討其在腦膠質瘤髮生髮展中的意義.方法 (1)取人腦膠質瘤組織標本72例,採用免疫組織化學染色檢測HAUSP、CD133的錶達,比較不同級彆膠質瘤HAUSP+細胞和CD133+細胞所佔百分比併分析其與膠質瘤級彆的相關性.(2)血清剝奪法從U87膠質瘤細胞株分離培養BTSCs,通過全反式維甲痠(ATRA)誘導分化,免疫細胞熒光雙重染色觀察HAUSP及CD133在不同時期錶達.結果 Ⅱ～Ⅳ級膠質瘤中HAUSP+細胞率分彆為(25.9±11.3)％、(49.8±19.8)％、(63.7±20.4)％;CD133+細胞率分彆為(12.60±3.49)％、(18.90±5.81)％、(24.80±7.54)％,不同級彆膠質瘤中HAUSP+細胞、CD133+細胞百分比差異均有統計學意義(P＜0.05),高級彆組膠質瘤中HAUSP+細胞、CD133+細胞錶達水平高于低級彆組,差異有統計學意義(P＜0.05);HAUSP+錶達和CD133+錶達呈正相關(r=0.915,P＜0.01);隨著U87膠質瘤榦細胞分化,HAUSP、CD133錶達逐漸減少.結論 HAUSP與榦細胞標誌物CD133之間有明顯的相關性.HAUSP在維持腦膠質瘤榦細胞未分化或低分化的狀態中可能起到重要作用.
목적 연구인뇌효질류중포진병독상관성범소특이성단백매(HAUSP)여뇌종류간세포(BTSCs)표지물CD133지간표체적관계,병탐토기재뇌효질류발생발전중적의의.방법 (1)취인뇌효질류조직표본72례,채용면역조직화학염색검측HAUSP、CD133적표체,비교불동급별효질류HAUSP+세포화CD133+세포소점백분비병분석기여효질류급별적상관성.(2)혈청박탈법종U87효질류세포주분리배양BTSCs,통과전반식유갑산(ATRA)유도분화,면역세포형광쌍중염색관찰HAUSP급CD133재불동시기표체.결과 Ⅱ～Ⅳ급효질류중HAUSP+세포솔분별위(25.9±11.3)％、(49.8±19.8)％、(63.7±20.4)％;CD133+세포솔분별위(12.60±3.49)％、(18.90±5.81)％、(24.80±7.54)％,불동급별효질류중HAUSP+세포、CD133+세포백분비차이균유통계학의의(P＜0.05),고급별조효질류중HAUSP+세포、CD133+세포표체수평고우저급별조,차이유통계학의의(P＜0.05);HAUSP+표체화CD133+표체정정상관(r=0.915,P＜0.01);수착U87효질류간세포분화,HAUSP、CD133표체축점감소.결론 HAUSP여간세포표지물CD133지간유명현적상관성.HAUSP재유지뇌효질류간세포미분화혹저분화적상태중가능기도중요작용.
Objective To explore the relationship between herpesvirus-associated ubiquitin-specific protease (HAUSP) and CD133 in gliomas of different pathological grades,and discuss the significance of HAUSP expression in glioma development.Methods The expression of HAUSP and CD133 were detected by immunohistochemistry in the specimens of 72 cases of glioma tissues.Brain tumor stem cells (BTSCs) were isolated from glioma celI line U87 and cultured in simplified serum-free neural stem cell medium by nanosphere suspension culture method spheres,and purified continuously through the monoclonal formation experiment.All-trans retinoic acid (ATRA) was used to induce the differentiation of BTSCs.Double immunofluorescence staining was used to detect the co-expressions of HAUSP and CD133.Results The percentages of HAUSP + and CD133 + in gliomas of grade Ⅱ-Ⅳ were (25.9 ± 11.3) ％,(49.8±19.8)％,(63.7±20.4)％,and (12.60±3.49)％,(18.90±5.81)％,(24.80±7.54)％ respectively,which were significantly different (P ＜ 0.05),and the expressions of HAUSP+,CD133 +cells in gliomas of grade Ⅲ and Ⅳ were significantly higher than those in glioma of grade Ⅱ (P ＜ 0.05).The HAUSP+ expression and CD133 + expression were positively correlated (r =0.915,P ＜ 0.01).HAUSP + and CD133 + expressions were reduced followed as the differentiation of BTSCs.Conclusion HAUSP and stem cell marker CD133 has a significant correlation,and they enjoy significant co-expressions in cellular level.HAUSP may play a crucial role in the maintenance of glioma stem cells undifferentiated or poorly differentiated state.
