中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
9期
1944-1946
,共3页
王建锋%王耿泽%宋展%厉冰
王建鋒%王耿澤%宋展%厲冰
왕건봉%왕경택%송전%려빙
胃癌%Krüpple样转录因子6%核组蛋白-2
胃癌%Krüpple樣轉錄因子6%覈組蛋白-2
위암%Krüpple양전록인자6%핵조단백-2
Gastric cancer%Krüppel-like factor 6%Nucleobindin-2
目的 观察在胃癌大鼠模型胃肠道组织中Krüpple样转录因子6(Klf-6)和核组蛋白-2(NUCB2)及相关基因表达的影响.方法 将Wister大鼠96只分为3组,A组32只,采用黄曲霉素B1(AFB1)对大鼠进行诱导处理3个月,制备胃癌大鼠模型.B组32只,应用AFB1对大鼠进行诱导处理6个月,制备胃癌大鼠模型.C组32只,为正常对照组.用免疫组织化学法检测3组大鼠胃肠道组织中NUCB2和Klf-6的表达水平.逆转录-聚合酶链反应(RT-PCR)技术检测两组大鼠胃肠道组织中NUCB2和Klf-6基因mRNA表达水平,比较其差异.结果 A、B组成功制备了胃癌大鼠模型,但不同诱导时间两组大鼠胃癌发病的程度和发病率不同,差异有统计学意义(P＜0.05).Klf-6和NUCB2在B组的表达率高于A组和C组,差异有统计学意义,A组Klf-6和NUCB2表达又高于C组,差异有统计学意义(x2 =3.17、4.95、3.79,P＜0.05).RT-PCR结果显示,B组胃肠道组织中Klf-6和NUCB2基因的mRNA表达水平高于A组,A组高于C组,差异有统计学意义(x2=2.98、4.13、3.52,P＜0.05).结论 肌氨酸乙酯盐酸盐诱导胃癌模型时间越长,胃癌大鼠胃肠道Klf-6和NUCB2的表达也越强.
目的 觀察在胃癌大鼠模型胃腸道組織中Krüpple樣轉錄因子6(Klf-6)和覈組蛋白-2(NUCB2)及相關基因錶達的影響.方法 將Wister大鼠96隻分為3組,A組32隻,採用黃麯黴素B1(AFB1)對大鼠進行誘導處理3箇月,製備胃癌大鼠模型.B組32隻,應用AFB1對大鼠進行誘導處理6箇月,製備胃癌大鼠模型.C組32隻,為正常對照組.用免疫組織化學法檢測3組大鼠胃腸道組織中NUCB2和Klf-6的錶達水平.逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測兩組大鼠胃腸道組織中NUCB2和Klf-6基因mRNA錶達水平,比較其差異.結果 A、B組成功製備瞭胃癌大鼠模型,但不同誘導時間兩組大鼠胃癌髮病的程度和髮病率不同,差異有統計學意義(P＜0.05).Klf-6和NUCB2在B組的錶達率高于A組和C組,差異有統計學意義,A組Klf-6和NUCB2錶達又高于C組,差異有統計學意義(x2 =3.17、4.95、3.79,P＜0.05).RT-PCR結果顯示,B組胃腸道組織中Klf-6和NUCB2基因的mRNA錶達水平高于A組,A組高于C組,差異有統計學意義(x2=2.98、4.13、3.52,P＜0.05).結論 肌氨痠乙酯鹽痠鹽誘導胃癌模型時間越長,胃癌大鼠胃腸道Klf-6和NUCB2的錶達也越彊.
목적 관찰재위암대서모형위장도조직중Krüpple양전록인자6(Klf-6)화핵조단백-2(NUCB2)급상관기인표체적영향.방법 장Wister대서96지분위3조,A조32지,채용황곡매소B1(AFB1)대대서진행유도처리3개월,제비위암대서모형.B조32지,응용AFB1대대서진행유도처리6개월,제비위암대서모형.C조32지,위정상대조조.용면역조직화학법검측3조대서위장도조직중NUCB2화Klf-6적표체수평.역전록-취합매련반응(RT-PCR)기술검측량조대서위장도조직중NUCB2화Klf-6기인mRNA표체수평,비교기차이.결과 A、B조성공제비료위암대서모형,단불동유도시간량조대서위암발병적정도화발병솔불동,차이유통계학의의(P＜0.05).Klf-6화NUCB2재B조적표체솔고우A조화C조,차이유통계학의의,A조Klf-6화NUCB2표체우고우C조,차이유통계학의의(x2 =3.17、4.95、3.79,P＜0.05).RT-PCR결과현시,B조위장도조직중Klf-6화NUCB2기인적mRNA표체수평고우A조,A조고우C조,차이유통계학의의(x2=2.98、4.13、3.52,P＜0.05).결론 기안산을지염산염유도위암모형시간월장,위암대서위장도Klf-6화NUCB2적표체야월강.
Objective To discuss the expression of Krüppel-like factor 6 (Klf-6) and nucleo-bindin-2 (NUCB2) genes in gastrointestinal tract of rats with gastric cancer.Methods Ninety-six adult Wister rats were randomly divided into three groups:32 rats each in groups A and B were drenched intraperitoneally with sarcosine ethyl ester hydrochloride to induce gastric cancer for 3 and 6 months respectively,and 32 rats in group C were injected with normal saline.The gastrointestinal tract was examined by electronic scale at 3rd and 6th month after modeling.The Klf-6 and NUCB2 expression in gastrointestinal tract of rats was observed,and the mRNA levels were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) after the rats were decapitated.The statistical analysis was done by using SPSS 14.0 statistical software.Results All the 96 rats were involved in the analysis of results.Positive rate of Klf-6 and NUCB2 in group A was higher than in groups B and C,and that in group B higher than in group C (x2 =3.17,4.95,and 3.79,P ＜0.05).RT-PCR revealed that the expression levels of Klf-6 and NUCB2 mRNA in group A were higher than in groups B and C,and those in group B higher than in group C (x2 =2.98,4.13 and 3.52,P ＜ 0.05).Conclusion Combined detection of the expression of Klf-6 and NUCB2 in gastric cancer may give some guideline for the clinical diagnosis,illness evaluation,treatment and prognosis of gastric cancer.
