CAJ | 학술논문

目的比较两种经典的大鼠肾病模型的制模方法及评价指标,为肾病模型的研究提供参考数据.方法 30只雄性SD大鼠被随机分为对照组,嘌呤霉素氨基核苷组(PAN组)和阿霉素组(ADR组).比较3组大鼠的制模方法,评价指标(包括不同时间点大鼠体质量,蛋白尿水平,血清白蛋白浓度,胆固醇浓度,肌酐和尿素氮水平,电镜下足突融合程度等),制模药品不良反应及实验费用等指标,评价两种模型的优缺点.结果 (1)成模后PAN组大鼠体质量,毛色等均无明显变化；ADR组表现为脱毛、毛色差、大便稀,且体质量显著低于对照组(P＜0.01).(2)PAN组制模第10天起24 h尿蛋白量显著高于对照组并持续至第15天(P＜0.01)；ADR组制模第15天始24 h尿蛋白量显著高于对照组并持续至第21天(P＜0.01).(3)PAN组制模第10天起血清白蛋白水平显著低于对照组(P＜0.01),制模第15天恢复；血清胆固醇水平在制模第10天显著高于对照组(P＜0.01),第15天恢复；ADR组血清白蛋白浓度在制模第15天显著低于对照组(P＜0.05),制模第21天恢复；两组大鼠不同时间点血肌酐和尿素氮浓度与对照组差异无统计学意义.(4)PAN组在制模第10天足突宽度显著宽于对照组(P＜0.01)；ADR组在第21天时足突宽度显著宽于对照组(P＜0.05)；(5)PAN组成功诱导1只大鼠(100 g)肾病模型费用为ADR组的3.1倍(578.10元比186.94元).结论两种药物均可成功诱导大鼠肾病模型.PAN有制模方便、不良反应小、组间差异小、急性期发病快、周期短等优点,但价格昂贵；ADR给药途径较困难,不良反应及组间差异大,价格相对便宜.
목적 비교량충경전적대서신병모형적제모방법급평개지표,위신병모형적연구제공삼고수거.방법 30지웅성SD대서피수궤분위대조조,표령매소안기핵감조(PAN조)화아매소조(ADR조).비교3조대서적제모방법,평개지표(포괄불동시간점대서체질량,단백뇨수평,혈청백단백농도,담고순농도,기항화뇨소담수평,전경하족돌융합정도등),제모약품불량반응급실험비용등지표,평개량충모형적우결점.결과 (1)성모후PAN조대서체질량,모색등균무명현변화；ADR조표현위탈모、모색차、대편희,차체질량현저저우대조조(P＜0.01).(2)PAN조제모제10천기24 h뇨단백량현저고우대조조병지속지제15천(P＜0.01)；ADR조제모제15천시24 h뇨단백량현저고우대조조병지속지제21천(P＜0.01).(3)PAN조제모제10천기혈청백단백수평현저저우대조조(P＜0.01),제모제15천회복；혈청담고순수평재제모제10천현저고우대조조(P＜0.01),제15천회복；ADR조혈청백단백농도재제모제15천현저저우대조조(P＜0.05),제모제21천회복；량조대서불동시간점혈기항화뇨소담농도여대조조차이무통계학의의.(4)PAN조재제모제10천족돌관도현저관우대조조(P＜0.01)；ADR조재제21천시족돌관도현저관우대조조(P＜0.05)；(5)PAN조성공유도1지대서(100 g)신병모형비용위ADR조적3.1배(578.10원비186.94원).결론 량충약물균가성공유도대서신병모형.PAN유제모방편、불량반응소、조간차이소、급성기발병쾌、주기단등우점,단개격앙귀；ADR급약도경교곤난,불량반응급조간차이대,개격상대편의.
Objective To compared two classical rat models of nephrotic syndrome and to provide some reference data to researchers.Methods Thirty male SD rats were randomly divided into control group,puromycin aminonucleoside-induced nephrotic syndrome (PAN) group and adriamycininduced nephrotic syndrome (ADR) group.The body weight,twenty four hour proteinuria level,serum albumin concentration,cholesterol concentration,creatinine and urea concentration were measured.The renal pathology change was evaluated.The drug toxic effects,administration methods and the costs were also compared.Results There was no significant difference in body weight and hair color between control group and PAN group.Compared to control group,the body weight of the rats significantly decreased at day 15 and day 21 in ADR group (P ＜ 0.01),accompanied by epilation and diarrhea.Compared to control group,the 24-hour urinary protein levels increased significantly at day 10 (P ＜ 0.01),day 15 (P ＜ 0.01),and reached the peak level at day 15 (P ＜ 0.01),day 21 (P ＜ 0.01) in PAN group and ADR group respectively.Compared to control group,the serum albumin concentration decreased significantly at day 10 (P＜0.01),and return to normal level at day 15.The serum cholesterol concentration was increased significantly at day 10 (P ＜ 0.01) and return to normal at day 15 in PAN group.Compared to control group,the serum albumin concentration was decreased significantly at day 15 (P＜0.05) and return to normal at day 21 in ADR group.No significant difference of serum creatinine and serum urea nitrogen levels were found among three groups.Compared to control group,the width of foot process increased significantly at day10 (P ＜ 0.01) and day 15 (P ＜ 0.05) in PAN group and ADR group respectively.To successfully induce a nephrotic rat model (per 100 g),the cost of PAN group was 3.1 times of ADR group (578.10 yuan vs 186.94 yuan).Conclusions Nephrotic syndrome can be induced by both PAN and ADR.The administration of PAN via intraperitoneal injection is more convenient as compared to ADR via tail intravenous injection.Compared to ADR,PAN can induce nephrotic syndrome model more rapidly,with more consistent detection index,and less toxic effects,but its cost is more expensive.