CAJ | 학술논문

目的通过检测肽酰基精氨酸脱亚胺酶4(PAD4)在ANCA相关性小血管炎(AAV)患者血清中的水平,探讨其在AAV发病中的作用及意义.方法收集13例AAV患者发作期与缓解期血清,11例原发性慢性肾脏病患者的血清,12例健康对照的血清.用酶联免疫吸附法(ELISA)检测PAD4的水平,分析其与AAV患者BVAS评分的关系.结果 (1)在AAV组,PAD4在发作期与缓解期的水平明显高于健康对照组;而在慢性肾脏病患者组中,PAD4水平与健康对照相比无显著差异.(2)AAV组有肾损害的患者PAD4水平,无论发作期还是缓解期,均高于慢性肾脏病组患者的PAD4水平.(3)AAV肾损害组患者发作期的PAD4水平与BVAS评分呈显著正相关(r=0.71,P=0.02).结论 PAD4参与了AAV的发生和发展.
목적 통과검측태선기정안산탈아알매4(PAD4)재ANCA상관성소혈관염(AAV)환자혈청중적수평,탐토기재AAV발병중적작용급의의.방법 수집13례AAV환자발작기여완해기혈청,11례원발성만성신장병환자적혈청,12례건강대조적혈청.용매련면역흡부법(ELISA)검측PAD4적수평,분석기여AAV환자BVAS평분적관계.결과 (1)재AAV조,PAD4재발작기여완해기적수평명현고우건강대조조;이재만성신장병환자조중,PAD4수평여건강대조상비무현저차이.(2)AAV조유신손해적환자PAD4수평,무론발작기환시완해기,균고우만성신장병조환자적PAD4수평.(3)AAV신손해조환자발작기적PAD4수평여BVAS평분정현저정상관(r=0.71,P=0.02).결론 PAD4삼여료AAV적발생화발전.
Objective To explore the significance of peptidylarginine deiminase type 4 (PAD4) in the pathogenesis of ANCA-associated vasculitis (AAV) by detecting its level in patients with AAV.Methods Sera from 13 patients with AAV,11 patients with primary chronic kidney disease and 12 healthy controls were collected.Serum PAD4 was detected using commercial ELISA kits.The association between serum PAD4 and BVAS of AAV was further investigated.Results (1) The serum level of PAD4 in patients with AAV in active and remission stages were all higher than that in the healthy controls.The serum level of PAD4 in patients with CKD was not found elevated compared with the normal controls.(2) The serum levels of PAD4 in AAV with renal damage were all significantly higher than that in CKD group no matter in active or remission stage.(3) The serum level of PAD4 in AAV with renal damage in active stage was positively correlated with BVAS (r=0.71,P=0.02).Conclusion PAD4 is involved in the pathogenesis of AAV.