中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2014年
6期
437-442
,共6页
郑茜子%屈磊%唐嘉薇%孟立强%王玉
鄭茜子%屈磊%唐嘉薇%孟立彊%王玉
정천자%굴뢰%당가미%맹립강%왕옥
NADPH氧化酶%输尿管梗阻%纤维化%夹竹桃麻素
NADPH氧化酶%輸尿管梗阻%纖維化%夾竹桃痳素
NADPH양화매%수뇨관경조%섬유화%협죽도마소
NADPH oxidase%Ureteral obstruction%Fibrosis%Apocynin
目的 探讨NADPH氧化酶系统(NOXs)对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响.方法 雄性Wistar大鼠按随机区间法分为假手术组(Sham,n=8)、假手术+夹竹桃麻素(apocynin)治疗组(SA,n=8)、UUO组(n=8)和UUO+夹竹桃麻素治疗组(UA,n=8).SA及UA组自手术日起每天给予夹竹桃麻素100 mg/kg灌胃治疗,Sham组和UUO组给予等量清水灌胃.术后第7天处死取材,采用ELISA及Western印迹法分别检测肾脏组织超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,8-异构前列腺素F2α(8-iso-PGF2α)浓度,NOX2、NOX4及α平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原(COL-Ⅰ)的表达,ERK1/2激酶的活化.结果 与Sham组相比,UUO大鼠第7天NOX2、NOX4表达及8-iso-PGF2α水平显著升高(P＜0.05),同时伴有肾组织COL-Ⅰ和α-SMA表达升高、ERK1/2激酶活化增加(P＜0.05).夹竹桃麻素治疗显著降低NOX2、NOX4的表达及8-iso-PGF2α水平(P＜0.05),同时大鼠肾组织α-SMA及COL-Ⅰ表达显著下降、ERK1/2活化降低(P＜0.05).夹竹桃麻素治疗对肾组织SOD及CAT活性无明显影响.结论 NADPH氧化酶系统通过促氧化应激参与梗阻性肾病大鼠肾间质纤维化的进展,抑制该系统可能作为抑制肾间质纤维化的治疗靶点.
目的 探討NADPH氧化酶繫統(NOXs)對單側輸尿管梗阻(UUO)大鼠腎間質纖維化的影響.方法 雄性Wistar大鼠按隨機區間法分為假手術組(Sham,n=8)、假手術+夾竹桃痳素(apocynin)治療組(SA,n=8)、UUO組(n=8)和UUO+夾竹桃痳素治療組(UA,n=8).SA及UA組自手術日起每天給予夾竹桃痳素100 mg/kg灌胃治療,Sham組和UUO組給予等量清水灌胃.術後第7天處死取材,採用ELISA及Western印跡法分彆檢測腎髒組織超氧化物歧化酶(SOD)和過氧化氫酶(CAT)活性,8-異構前列腺素F2α(8-iso-PGF2α)濃度,NOX2、NOX4及α平滑肌肌動蛋白(α-SMA)、Ⅰ型膠原(COL-Ⅰ)的錶達,ERK1/2激酶的活化.結果 與Sham組相比,UUO大鼠第7天NOX2、NOX4錶達及8-iso-PGF2α水平顯著升高(P＜0.05),同時伴有腎組織COL-Ⅰ和α-SMA錶達升高、ERK1/2激酶活化增加(P＜0.05).夾竹桃痳素治療顯著降低NOX2、NOX4的錶達及8-iso-PGF2α水平(P＜0.05),同時大鼠腎組織α-SMA及COL-Ⅰ錶達顯著下降、ERK1/2活化降低(P＜0.05).夾竹桃痳素治療對腎組織SOD及CAT活性無明顯影響.結論 NADPH氧化酶繫統通過促氧化應激參與梗阻性腎病大鼠腎間質纖維化的進展,抑製該繫統可能作為抑製腎間質纖維化的治療靶點.
목적 탐토NADPH양화매계통(NOXs)대단측수뇨관경조(UUO)대서신간질섬유화적영향.방법 웅성Wistar대서안수궤구간법분위가수술조(Sham,n=8)、가수술+협죽도마소(apocynin)치료조(SA,n=8)、UUO조(n=8)화UUO+협죽도마소치료조(UA,n=8).SA급UA조자수술일기매천급여협죽도마소100 mg/kg관위치료,Sham조화UUO조급여등량청수관위.술후제7천처사취재,채용ELISA급Western인적법분별검측신장조직초양화물기화매(SOD)화과양화경매(CAT)활성,8-이구전렬선소F2α(8-iso-PGF2α)농도,NOX2、NOX4급α평활기기동단백(α-SMA)、Ⅰ형효원(COL-Ⅰ)적표체,ERK1/2격매적활화.결과 여Sham조상비,UUO대서제7천NOX2、NOX4표체급8-iso-PGF2α수평현저승고(P＜0.05),동시반유신조직COL-Ⅰ화α-SMA표체승고、ERK1/2격매활화증가(P＜0.05).협죽도마소치료현저강저NOX2、NOX4적표체급8-iso-PGF2α수평(P＜0.05),동시대서신조직α-SMA급COL-Ⅰ표체현저하강、ERK1/2활화강저(P＜0.05).협죽도마소치료대신조직SOD급CAT활성무명현영향.결론 NADPH양화매계통통과촉양화응격삼여경조성신병대서신간질섬유화적진전,억제해계통가능작위억제신간질섬유화적치료파점.
Objective To clarify whether the NADPH oxidases (NOXs) family contributed to the reactive oxygen species (ROS) production and subsequent interstitial fibrosis in unilateral ureter obstruction (UUO) rats.Methods Male Wistar rats were randomly divided into sham operation group (n =8),sham operation + apocynin treatment group (n =8),UUO operation group (n =8) and UUO operation+apocynin treatment group (n =8).Either vehicle or apocynin (100 mg/kg per day) were given by gavage for 7 days after surgery.Rats were sacrificed at 7th day.ELISA was used to detect the activity of superoxide dismutase (SOD) and catalase (CAT),and the level of 8-iso-prostaglandin F2alpha (8-isoPGF2α) in renal tissue.Western blotting was used to detect the protein expressions of NADPH oxidase subunit NOX2 and NOX4,α-smooth muscle actin(α-SMA),collagen Ⅰ (COL-Ⅰ) and the level of ERK1/ 2 phosphorylation (p-ERK1/2).Results UUO rats with vehicle displayed increased oxidative stress,as measured by renal tissue 8-iso-PGF2α,accompanied with increased renal expression of NADPH oxidases (NOX2,1.5-fold and NOX4,1.7-fold,respectively),compared with sham-operated rats (P ＜0.05).Furthermore,vehicle treated UUO rats showed increased renal COL-Ⅰ and α-SMA levels,compared with sham-operated rats (P ＜ 0.05).ERK1/2 was also activated as detected by p-ERK1/2 expression in UUO rats with vehicle (P ＜ 0.05).Apocynin treatment significantly decreased renal tissue 8-iso-PGF2α level and expressions of NOX2 (-28.7％) and NOX4 (-31.0％) in UUO rats,respectively,compared with vehicle treated rats (P ＜ 0.05).And significant decrease of COL-Ⅰ (-26.4％) and α-SMA expression (-80.0％) were also observed (P ＜ 0.05).The activation of ERK1/2 in UUO rats was greatly inhibited by apocynin treatment (P ＜ 0.01).Despite the pronounced dysregulation of pro-oxidative NOXs family,no compensatory increase of antioxidative enzyme activities occurred.Conclusion The NOXs family contributes largely to the production of ROS and subsequent interstitial fibrosis after ureter ligation,and inhibition of the NOXs family may be a choice for preventing interstitial fibrosis.
