CAJ | 학술논문

目的探讨氟伐他汀对腹膜透析大鼠腹膜组织转化生长因子β1(TGF-β1)表达的影响.方法每日腹腔注射2.5％或4.25％的葡萄糖腹透液建立腹膜透析大鼠模型.SD雄性大鼠(体质量180 ～ 200 g)随机分为7个组:(1)正常对照组;(2)生理盐水组;(3)氟伐他汀组;(4) 2.5％PD组;(5)4.25％PD组;(6)氟伐他汀干预的2.5％PD组;(7)氟伐他汀干预的4.25％PD组.6周后留取大鼠壁层腹膜组织,光镜观察各组大鼠壁层腹膜形态学的变化,RT-PCR法及免疫组化法分别测定各组大鼠腹膜TGF-β1及纤维连接蛋白(fibronectin,FN)的mRNA及蛋白的表达量.结果光镜下Masson染色显示4.25％PD组与2.5％PD组大鼠腹膜较正常对照组明显增厚;氟伐他汀干预的42％PD组和2.5％PD组的壁层腹膜厚度薄于相应未干预的PD组,但仍厚于正常对照组;生理盐水组和氟伐他汀组大鼠腹膜的厚度较正常对照组未见明显改变.2.5％PD组与4.25％PD组的TGF-β1和FN的mRNA及蛋白的表达量均明显高于正常对照组(均P＜ 0.05),氟伐他汀干预的2.5％PD组和4.25％PD组的TGF-β1和FN的mRNA及蛋白表达量较无氟伐他汀干预的2.5％PD组和4.25％PD组均有显著减少(均P＜0.05).生理盐水组和氟伐他汀组大鼠腹膜TGF-β1和FN的mRNA及蛋白表达量与正常组比较,差异无统计学意义.结论氟伐他汀能改善高糖腹透液环境下大鼠腹膜的厚度,同时降低高糖腹透液诱导的大鼠腹膜TGF-β1和FN mRNA及蛋白的表达,对腹膜有保护作用.
목적 탐토불벌타정대복막투석대서복막조직전화생장인자β1(TGF-β1)표체적영향.방법 매일복강주사2.5％혹4.25％적포도당복투액건립복막투석대서모형.SD웅성대서(체질량180 ～ 200 g)수궤분위7개조:(1)정상대조조;(2)생리염수조;(3)불벌타정조;(4) 2.5％PD조;(5)4.25％PD조;(6)불벌타정간예적2.5％PD조;(7)불벌타정간예적4.25％PD조.6주후류취대서벽층복막조직,광경관찰각조대서벽층복막형태학적변화,RT-PCR법급면역조화법분별측정각조대서복막TGF-β1급섬유련접단백(fibronectin,FN)적mRNA급단백적표체량.결과 광경하Masson염색현시4.25％PD조여2.5％PD조대서복막교정상대조조명현증후;불벌타정간예적42％PD조화2.5％PD조적벽층복막후도박우상응미간예적PD조,단잉후우정상대조조;생리염수조화불벌타정조대서복막적후도교정상대조조미견명현개변.2.5％PD조여4.25％PD조적TGF-β1화FN적mRNA급단백적표체량균명현고우정상대조조(균P＜ 0.05),불벌타정간예적2.5％PD조화4.25％PD조적TGF-β1화FN적mRNA급단백표체량교무불벌타정간예적2.5％PD조화4.25％PD조균유현저감소(균P＜0.05).생리염수조화불벌타정조대서복막TGF-β1화FN적mRNA급단백표체량여정상조비교,차이무통계학의의.결론 불벌타정능개선고당복투액배경하대서복막적후도,동시강저고당복투액유도적대서복막TGF-β1화FN mRNA급단백적표체,대복막유보호작용.
Objective To investigate the effect of fluvastatin on TGF-β1 expression in a rat model of peritoneal dialysis(PD).Methods A rat model of PD was built by intraperitoneal injection of 2.5％ or 4.25％ peritoneal dialysate.SD rats were randomly divided into 7 groups:(1) Normal control group; (2)Saline control group:saline 100 ml/kg intraperitoneal injection(IP) each day; (3) Fluvastatin treatment group:fluvastatin intragastrically administration 10 mg/kg each day; (4) 2.5％ PD group:2.5％ peritoneal dialysate IP 100 ml/kg everyday; (5)4.25％ PD group:4.25％ peritoneal dialysate IP 100 ml/kg everyday; (6)2.5％ PD plus fluvastatin treatment group:2.5％ peritoneal dialysate IP 100 ml/ kg plus fluvastatin 10 mg/kg everyday; (7)4.25％ PD plus fluvastatin treatment group:4.25％ peritoneal dialysate IP 100 ml/kg plus fluvastatin 10 mg/kg everyday.The rats were sacrificed at 6 weeks and peritoneal tissues were dissected.The expressions of TGF-β1 and FN were examined by RT-PCR and immunohistochemical analysis.Masson staining was used for histological examination.Results Masson staining showed that the peritoneum thickened in 2.5％ and 4.25％ PD group than in normal control group and saline control group.The fluvastatin treatment ameliorated the thickening of peritoneum induced by PD.RT-PCR and immunohistochemical analysis showed that the mRNA and protein expression of TGF-β1 and FN increased in 2.5％ and 4.25％ PD group than in normal and saline control group (all P ＜ 0.05).The fluvastatin treatment ameliorated the increased expression of TGF-β1 and FN induced by PD.There was no statistically significant difference among normal control group,saline control group and fluvastatin treatment group in both peritoneal thickness and the expression of TGF-β1 and FN.Conclusion Fluvastatin can reduce the increased expressions of TGF -β1 and FN in rat peritoneum and ameliorate the thickening of peritoneum induced by PD.