中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2014年
8期
592-597
,共6页
姜祥品%郭红岩%宝群%崔美玉
薑祥品%郭紅巖%寶群%崔美玉
강상품%곽홍암%보군%최미옥
抗原,CD26%糖尿病%肾疾病%霉酚酸酯
抗原,CD26%糖尿病%腎疾病%黴酚痠酯
항원,CD26%당뇨병%신질병%매분산지
Antigens,CD26%Diabetes mellitus%Kidney diseases%Mycophenolate mofetil
目的 探讨CD26(又称为二肽基肽酶Ⅳ)在糖尿病(DM)大鼠肾组织中的表达及霉酚酸酯(MMF)对糖尿病大鼠肾组织CD26的影响.方法 将Wistar大鼠按随机数字表法分为正常对照组、DM模型组、MMF治疗组,每组7只.以链脲菌素腹膜内注射加高脂高糖饮食制备2型DM动物模型.16周后,检测各组大鼠血糖(BG)、血尿素氮(BUN)、血肌酐(Scr)、肾肥大指数[肾质量(KW)/体质量(BW)]和24 h尿蛋白量(24Upro);用流式细胞术检测肾组织中CD3+、CD4+T淋巴细胞的含量;Western印迹、免疫组化法检测肾组织CD26的表达.结果 与对照组相比,DM组大鼠BG、BUN、Scr、24Upro等指标均显著升高(P<0.05);除BG、KW/BW外,MMF治疗组上述指标均较DM组低(P<0.05).DM组大鼠肾脏内CD3+、CD4+T细胞较对照组显著上调(P<0.01).CD26主要定位于肾脏间质T淋巴细胞;CD26在DM组大鼠肾组织的表达较对照组明显增强,MMF治疗组其表达显著下降(P<0.05).DM组大鼠肾间质CD26+T细胞浸润与24Upro呈正相关(r2=0.770,P<0.05).结论 CD26与糖尿病肾病具有相关性,MMF可能通过抑制T淋巴细胞增殖以减少肾脏间质CD26的表达,从而起到肾脏保护作用.
目的 探討CD26(又稱為二肽基肽酶Ⅳ)在糖尿病(DM)大鼠腎組織中的錶達及黴酚痠酯(MMF)對糖尿病大鼠腎組織CD26的影響.方法 將Wistar大鼠按隨機數字錶法分為正常對照組、DM模型組、MMF治療組,每組7隻.以鏈脲菌素腹膜內註射加高脂高糖飲食製備2型DM動物模型.16週後,檢測各組大鼠血糖(BG)、血尿素氮(BUN)、血肌酐(Scr)、腎肥大指數[腎質量(KW)/體質量(BW)]和24 h尿蛋白量(24Upro);用流式細胞術檢測腎組織中CD3+、CD4+T淋巴細胞的含量;Western印跡、免疫組化法檢測腎組織CD26的錶達.結果 與對照組相比,DM組大鼠BG、BUN、Scr、24Upro等指標均顯著升高(P<0.05);除BG、KW/BW外,MMF治療組上述指標均較DM組低(P<0.05).DM組大鼠腎髒內CD3+、CD4+T細胞較對照組顯著上調(P<0.01).CD26主要定位于腎髒間質T淋巴細胞;CD26在DM組大鼠腎組織的錶達較對照組明顯增彊,MMF治療組其錶達顯著下降(P<0.05).DM組大鼠腎間質CD26+T細胞浸潤與24Upro呈正相關(r2=0.770,P<0.05).結論 CD26與糖尿病腎病具有相關性,MMF可能通過抑製T淋巴細胞增殖以減少腎髒間質CD26的錶達,從而起到腎髒保護作用.
목적 탐토CD26(우칭위이태기태매Ⅳ)재당뇨병(DM)대서신조직중적표체급매분산지(MMF)대당뇨병대서신조직CD26적영향.방법 장Wistar대서안수궤수자표법분위정상대조조、DM모형조、MMF치료조,매조7지.이련뇨균소복막내주사가고지고당음식제비2형DM동물모형.16주후,검측각조대서혈당(BG)、혈뇨소담(BUN)、혈기항(Scr)、신비대지수[신질량(KW)/체질량(BW)]화24 h뇨단백량(24Upro);용류식세포술검측신조직중CD3+、CD4+T림파세포적함량;Western인적、면역조화법검측신조직CD26적표체.결과 여대조조상비,DM조대서BG、BUN、Scr、24Upro등지표균현저승고(P<0.05);제BG、KW/BW외,MMF치료조상술지표균교DM조저(P<0.05).DM조대서신장내CD3+、CD4+T세포교대조조현저상조(P<0.01).CD26주요정위우신장간질T림파세포;CD26재DM조대서신조직적표체교대조조명현증강,MMF치료조기표체현저하강(P<0.05).DM조대서신간질CD26+T세포침윤여24Upro정정상관(r2=0.770,P<0.05).결론 CD26여당뇨병신병구유상관성,MMF가능통과억제T림파세포증식이감소신장간질CD26적표체,종이기도신장보호작용.
Objective To investigate the expression of CD26 (dipeptidyl peptidase 4) in the kidney tissues of diabetic rats and the effects of mycophenolate mofetil (MMF) on the renal CD26 expression.Methods Wistar rats were randomly divided into three groups:normal control group (NC group,n=7),diabetic model group (DM group,n=7) and MMF-treated group (MMF group,n=7).Wistar rats were fed with high-sucrose-high-fat diet and injected with streptozotocin into abdominal cavity to induce diabetes.Sixteen weeks later,blood glucose (BG),blood urea nitrogen (BUN),serum creatinine (Scr),renal hypertrophy index (kidney weight]body weight) and 24 hour urinary protein (24Upro) were measured.The number of CD37CD4+ T cells in renal tissues were measured through flow cytometry.The expression of CD26 in kidney was examined by using Western blotting and immunohistochemistry.Results Compared with NC group,BG,BUN,Scr,kidney weight/body weight,24Upro were significantly increased in DM group (P < 0.05).Except BG and kidney weight] body weight,the above-mentioned parameters were lower in MMF group compared with that in DM group (P < 0.05).Intrarenal CD37CD4+ T cells were significantly up-regulated in DM group compared with that in NC group (P < 0.01).CD26 in renal tissue was mainly expressed in T lymphocytes of renal interstitium.CD26 expression in DM group was significantly higher than that in NC group,and also higher than that in MMF group (P < 0.05).In DM group,CD26+ T lymphocytes infiltration of renal interstitium was positively correlated with 24Upro (r2=0.770,P < 0.05).Conclusions CD26 is related with diabetic nephropathy.MMF maybe inhibit T lymphocytes infiltration to reduce the expression of CD26 in renal interstitium,thus protecting the kidney function.