CAJ | 학술논문

目的观察雷公藤多甙联合厄贝沙坦对糖尿病肾病大鼠足细胞nephrin和podocinmRNA和蛋白表达的影响.方法将体重200 ～ 260 g的50只SD大鼠按随机数字表法分为正常对照组和4个模型组(n=10),后者分别应用链脲佐菌素造模成功后分为糖尿病肾病组(DN组)、厄贝沙坦组、雷公藤多甙组和雷公藤多甙联合厄贝沙坦组.给予相应干预8周后,检测血尿指标,HE染色观察肾组织病理变化,逆转录-多聚酶链反应(RT-PCR)法检测各组大鼠肾皮质中nephrin和podocinmRNA和蛋白表达.结果 (1)与DN组大鼠比较,雷公藤多甙联合厄贝沙坦组大鼠肾皮质nephrin mRNA (0.507±0.024比0.276 ±0.015,P ＜0.01)和podocin mRNA (0.533±0.024比0.463±0.022,P＜0.01)表达上调.(2)与DN组大鼠比较,雷公藤多甙联合厄贝沙坦组大鼠肾皮质nephrin蛋白(0.738±0.029比0.199±0.012,P＜0.01)和podocin蛋白(0.811 ±0.032比0.227±0.014,P＜0.01)表达上调.(3) HE染色和Masson染色显示,糖尿病组大鼠肾脏肾小球体积增大,系膜基质弥漫增多,系膜细胞明显增多,基底膜弥漫增厚,间质可见灶性淋巴细胞及单核细胞浸润,厄贝沙坦组和雷公藤多甙组病变较糖尿病组减轻,雷公藤多甙联合厄贝沙坦组大鼠肾脏组织病变较厄贝沙坦组和雷公藤多甙组进一步减轻.结论雷公藤多甙联合厄贝沙坦对糖尿病大鼠的肾脏足细胞具有保护作用,这种保护作用可能是通过上调足细胞nephrin和podocin mRNA和蛋白表达有关.
목적 관찰뢰공등다대연합액패사탄대당뇨병신병대서족세포nephrin화podocinmRNA화단백표체적영향.방법 장체중200 ～ 260 g적50지SD대서안수궤수자표법분위정상대조조화4개모형조(n=10),후자분별응용련뇨좌균소조모성공후분위당뇨병신병조(DN조)、액패사탄조、뢰공등다대조화뢰공등다대연합액패사탄조.급여상응간예8주후,검측혈뇨지표,HE염색관찰신조직병리변화,역전록-다취매련반응(RT-PCR)법검측각조대서신피질중nephrin화podocinmRNA화단백표체.결과 (1)여DN조대서비교,뢰공등다대연합액패사탄조대서신피질nephrin mRNA (0.507±0.024비0.276 ±0.015,P ＜0.01)화podocin mRNA (0.533±0.024비0.463±0.022,P＜0.01)표체상조.(2)여DN조대서비교,뢰공등다대연합액패사탄조대서신피질nephrin단백(0.738±0.029비0.199±0.012,P＜0.01)화podocin단백(0.811 ±0.032비0.227±0.014,P＜0.01)표체상조.(3) HE염색화Masson염색현시,당뇨병조대서신장신소구체적증대,계막기질미만증다,계막세포명현증다,기저막미만증후,간질가견조성림파세포급단핵세포침윤,액패사탄조화뢰공등다대조병변교당뇨병조감경,뢰공등다대연합액패사탄조대서신장조직병변교액패사탄조화뢰공등다대조진일보감경.결론 뢰공등다대연합액패사탄대당뇨병대서적신장족세포구유보호작용,저충보호작용가능시통과상조족세포nephrin화podocin mRNA화단백표체유관.
Objective To observe the effect of tripterygium and irbesartan on nephrin and podocin expression in the podocyte of diabetic nephropathy rats.Methods Fifty SD rats (weight 200-260 g) were randomly divided into control group(group C) and model group.Rats in model group were then divided into diabetic nephropathy group (group DN),Irbesartan group (group Ⅰ),Tripterygium group (group T) and combined treatment group(group T + I) after being given streptozotocin (STZ) (60 mg/kg) by a single intraperitoneal injection to establish animal model of diabetes.After 8 weeks,blood and Urine index were measured.The renal cortical tissues were obtained and used for HE staining to determine pathological changes.The expression of nephrin and podocin in renal cortices were semiquantitatively measured with RT-PCR.Results Compared to group DN,the mRNA expressions of nephrin and podocin were higher in the cortex of renal in group (T + I) compared to group DN (0.507 ± 0.024 vs 0.276 ± 0.015,P ＜ 0.01 and 0.533 ± 0.024 vs 0.463 ± 0.022,P ＜ 0.01).The expressions of nephrin and podocin protein were higher in the cortex of renal in group (T + I) compared to group DN (0.738 ± 0.029 vs 0.199 ± 0.012,P ＜ 0.01 and 0.811 ±0.032 vs 0.227 ±0.014,P ＜0.01).HE and Masson staining showed that the glomerular volume became bigger,messegium increased,mesangial cells proliferation,basement-membrane thickening and lymphocytes and mononuclear cell infiltration in group DN,which reduced in Irbesartan group and Tripterygium group,and further reducing in combined treatment group.Conclusions Tripterygium combined irbesartan play a protective role on the podocytes in diabetic nephropathy rats.The mechanism may be partly through the up-regulated expression of nephrin and podocin in kidney.