CAJ | 학술논문

目的探讨多氯联苯(polychlorinated biphenyl,PCB) 118对大鼠胰岛素敏感性的影响.方法将40只清洁级成年雄性Wistar大鼠[体重(200±10)g]按随机数字表法分为4组,每组10只,除正常对照组外,其余3组给予不同剂量PCB118:低、中、高剂量组分别为10、100、1000 μg· kg-1·d-1,每周腹腔注射5d,造模13周后行腹腔葡萄糖耐量试验(IPGTT),计算糖负荷后30 min胰岛素增值与血糖增值比值(△Ⅰ30/△G30)、葡萄糖、胰岛素曲线下面积(AUCg、AUCi)、AUCi/AUCg及稳态模型胰岛素抵抗指数(HOMA-IR).采用单因素方差分析进行多组间均数比较.结果低、中、高剂量组空腹胰岛素水平[(19.0±7.1)、(18.5±3.1)、(22.1±1.2) mU/L]、30 min胰岛素[(55.0±1.9)、(55.0±2.4)、(72.3±1.0) mU/L]及30 min血糖水平[(22.32 ±2.44)、(19.87±1.89)、(21.90±2.88) mmol/L]明显高于对照组[(10.05 ±3.59)、(25.68±1.27) mU/L、(17.08 ± 1.35) mmol/L],差异有统计学意义(F值分别为3.622、8.798、7.024,均P＜0.05)；高剂量组60、120 min胰岛素水平高于对照组[60 min(46.3±1.2)比(18.1±1.1)mU/L,t=3.287,P＜0.05;120 min(39.5±1.3)比(24.5 ±9.4) mU/L,t=2.435,P＜0.05],各剂量组△Ⅰ30/△G30、AUCi、AUCi/ AUCg明显高于对照组(F值分别为15.548、7.130、5.509,均P＜0.05),低、中、高剂量组HOMA-IR亦明显高于对照组(1.26±0.42、1.35±0.22、1.52±0.47比0.65±0.37,F=6.937,P＜0.05),上述指标在各剂量组间比较差异无统计学意义(均P＞0.05).结论低剂量PCB118持续暴露可能引起大鼠胰岛素敏感性降低.
목적 탐토다록련분(polychlorinated biphenyl,PCB) 118대대서이도소민감성적영향.방법 장40지청길급성년웅성Wistar대서[체중(200±10)g]안수궤수자표법분위4조,매조10지,제정상대조조외,기여3조급여불동제량PCB118:저、중、고제량조분별위10、100、1000 μg· kg-1·d-1,매주복강주사5d,조모13주후행복강포도당내량시험(IPGTT),계산당부하후30 min이도소증치여혈당증치비치(△Ⅰ30/△G30)、포도당、이도소곡선하면적(AUCg、AUCi)、AUCi/AUCg급은태모형이도소저항지수(HOMA-IR).채용단인소방차분석진행다조간균수비교.결과 저、중、고제량조공복이도소수평[(19.0±7.1)、(18.5±3.1)、(22.1±1.2) mU/L]、30 min이도소[(55.0±1.9)、(55.0±2.4)、(72.3±1.0) mU/L]급30 min혈당수평[(22.32 ±2.44)、(19.87±1.89)、(21.90±2.88) mmol/L]명현고우대조조[(10.05 ±3.59)、(25.68±1.27) mU/L、(17.08 ± 1.35) mmol/L],차이유통계학의의(F치분별위3.622、8.798、7.024,균P＜0.05)；고제량조60、120 min이도소수평고우대조조[60 min(46.3±1.2)비(18.1±1.1)mU/L,t=3.287,P＜0.05;120 min(39.5±1.3)비(24.5 ±9.4) mU/L,t=2.435,P＜0.05],각제량조△Ⅰ30/△G30、AUCi、AUCi/ AUCg명현고우대조조(F치분별위15.548、7.130、5.509,균P＜0.05),저、중、고제량조HOMA-IR역명현고우대조조(1.26±0.42、1.35±0.22、1.52±0.47비0.65±0.37,F=6.937,P＜0.05),상술지표재각제량조간비교차이무통계학의의(균P＞0.05).결론 저제량PCB118지속폭로가능인기대서이도소민감성강저.
Objective To investigate the effects of polychlorinated biphenyl (PCB)118 on the insulin sensitivity in rats.Methods Forty male Wistar rats were randomly divided into 4 groups:solvent control group,low dose,medium dose,high dose group (PCB118 0,10,100,1000 μg · kg-1 · d-1),respectively,with PCB118 intraperitoneally for five consecutive days per week.After 13 weeks,an intraperitoneal glucose tolerance test (IPGTT) was performed,and the ratio of increment insulin to glucose concentration at 30 min after the glucose loaded (△Ⅰ30/△G30),area under the curve (AUC) of glucose (AUCg),AUC of insulin (AUCi),AUCi/AUCg and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated.One-way analysis of variance (ANOVA) was used for data analysis.Results Compared with the controls,fasting insulin levels and insulin and blood glucose levels at IPGTT 30 min were significantly increased in all the PCB118-treated groups (fasting insulin levels:(19.0 ± 7.1),(18.5 ± 3.1),(22.1±1.2) vs (10.0±3.6) mU/L; insulin levels at IPGTT30 min:(55.0±1.9),(55.0± 2.4),(72.3±1.0) vs (25.7±1.3) mU/L; blood glucose levels at IPGTT30 min:(22.3 ±2.4),(19.87±1.89),(21.90±2.88) vs (17.08±1.35) mmol/L,F values were 3.622,8.798 and 7.024,respectively,all P ＜ 0.05).Insulin levels at IPGTT 60 min and 120 min were significantly increased only in high dose group (60 min:(46.2 ± 1.2) vs (18.1 ± 1.1) mU/L,t =3.287,P ＜ 0.05 ; 1 20 min:(39.5 ± 1.3) vs (24.5 ± 9.4) mU/L,t =2.435,P ＜ 0.05).AⅠ30/△G30,AUCi and AUCi/AUCg were dramatically increased in all the PCB118-treated groups (F values were 15.548,7.130 and 5.509,respectively,all P ＜ 0.05).HOMA-IR were also dramatically increased in all the PCB118-treated groups (1.26±0.42,1.35 ±0.22,1.52 ±0.47 vs 0.65 ±0.37,F=6.937,P＜0.05).However,these indicators showed no significant difference among the PCB118-treated groups (P ＞ 0.05).Conclusion Continuous exposure to a low dose of PCB118 could reduce insulin sensitivity in rats.