CAJ | 학술논문

目的探讨SLC2A9基因第八外显子137A/G多态性与糖尿病合并高尿酸血症的关系.方法纳入2009至2010年在青岛医学院附属医院门诊和病房的患者312例,其中糖尿病患者103例(男59例,女44例)、高尿酸血症患者105例(男86例,女19例)、糖尿病合并高尿酸血症患者104例(男63例,女41例),另选健康志愿者102名(男45名,女57名).采用聚合酶链反应-限制性片段长度多态性技术进行多态性检测.结果 137A/G位点最小等位基因频率为37.9％.137A/G位点多态性与高尿酸血症、糖尿病合并高尿酸血症发病相关(x2 =4.075、4.392,均P＜0.05),与单纯糖尿病无关(x2 =0.942,P＞0.05).校正性别、年龄因素后,logistic回归分析表明携带G等位基因的个体患高尿酸血症、糖尿病合并高尿酸血症的风险比携带A等位基因的个体高(OR=1.523、1.632,均P＜0.05),差异有统计学意义;与单纯糖尿病的发病风险无统计学意义(OR=1.121,P＞0.05).结论 SLC2A9基因第八外显子137A/G多态性与高尿酸血症、糖尿病合并高尿酸血症的发病密切相关,而与单纯糖尿病无关.
목적 탐토SLC2A9기인제팔외현자137A/G다태성여당뇨병합병고뇨산혈증적관계.방법 납입2009지2010년재청도의학원부속의원문진화병방적환자312례,기중당뇨병환자103례(남59례,녀44례)、고뇨산혈증환자105례(남86례,녀19례)、당뇨병합병고뇨산혈증환자104례(남63례,녀41례),령선건강지원자102명(남45명,녀57명).채용취합매련반응-한제성편단장도다태성기술진행다태성검측.결과 137A/G위점최소등위기인빈솔위37.9％.137A/G위점다태성여고뇨산혈증、당뇨병합병고뇨산혈증발병상관(x2 =4.075、4.392,균P＜0.05),여단순당뇨병무관(x2 =0.942,P＞0.05).교정성별、년령인소후,logistic회귀분석표명휴대G등위기인적개체환고뇨산혈증、당뇨병합병고뇨산혈증적풍험비휴대A등위기인적개체고(OR=1.523、1.632,균P＜0.05),차이유통계학의의;여단순당뇨병적발병풍험무통계학의의(OR=1.121,P＞0.05).결론 SLC2A9기인제팔외현자137A/G다태성여고뇨산혈증、당뇨병합병고뇨산혈증적발병밀절상관,이여단순당뇨병무관.
Objective To investigate the association between the 137A/G polymorphism of SLC2A9 gene and diabetes mellitus (DM) complicated hyperuricemia (HUA).Methods A total of 102 healthy controls (45 males and 57 females),103 patients with DM (59 males and 44 females),105 patients with HUA (86 males and 19 females),and 104 patients with DM complicated HUA (63 males and 41 females) were enrolled in the study from the Affiliated Hospital of Medical College of Qingdao University in 2009-2010.The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was used to detect the polymorphism.Results The minor allele frequency of 137A/G was 37.9％.Chi-square test revealed that 137A/G polymorphism was both significantly associated with HUA as well as DM complicated HUA(x2 =4.075,4.392,both P ＜ 0.05),but not with pure DM (x2 =0.942,P ＞0.05).After adjustment for age and gender,logistic regression analysis showed that people with G allele had higher risk of HUA and DM complicated HUA than those without G allele (OR =1.523,1.632,both P ＜ 0.005),no association with pure DM(OR =1.121,P ＞ 0.05).Conclusion The 137A/G polymorphism of SLC2A9 gene is closely associated with HUA as well as DM complicated HUA,but not with DM.