中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2013年
11期
674-677
,共4页
徐俊%王鹏华%褚月颉%丁敏%田应芳%冯书红%于德民%董晓庆%常津
徐俊%王鵬華%褚月頡%丁敏%田應芳%馮書紅%于德民%董曉慶%常津
서준%왕붕화%저월힐%정민%전응방%풍서홍%우덕민%동효경%상진
糖尿病%表皮生长因子%纳米微粒%表皮生长因子受体%创面愈合
糖尿病%錶皮生長因子%納米微粒%錶皮生長因子受體%創麵愈閤
당뇨병%표피생장인자%납미미립%표피생장인자수체%창면유합
Diabetes mellitus%Epidermal growth factor%Nanoparticle%Epidermal growth factor receptor%Wound healing
目的 观察表皮生长因子(EGF)纳米微粒对糖尿病大鼠皮肤创面的愈合作用,探讨EGF纳米微粒促进愈合的机制.方法 制备EGF纳米微粒,测定EGF纳米微粒载药率、包封率和释药行为.用打孔器制备糖尿病大鼠皮肤创面模型.分为4组:EGF纳米组(给予含1μg/d EGF的纳米微粒),EGF组(给予1μg/d EGF),空微粒组(给予不含EGF纳米微粒),磷酸盐缓冲液(PBS)对照组,于3、7、14、21 d照相并取材,计算创面愈合率并比较创面病理学变化及EGF受体阳性细胞面积.结果 EGF纳米微粒平均粒径为193.5 nm,载药率为0.02％,包封率为85％.药物释放达24h.EGF纳米组创面愈合比EGF组快[14 d,(93.8±1.8)％比(89.3±2.3)％,P＜0.05;21 d(96.6±1.6)％比(92.1±4.3)％,P＜0.05].EGF纳米组创面内EGF受体阳性细胞面积高于EGF组[7d,(49.4±6.5)％比(35.1±2.8)％,P ＜0.05;14 d,(80.2±3.8)％比(73.4±1.7)％,P＜0.05].结论 EGF纳米微粒上调创面内细胞EGF受体表达,加速创面的愈合,效果优于EGF.
目的 觀察錶皮生長因子(EGF)納米微粒對糖尿病大鼠皮膚創麵的愈閤作用,探討EGF納米微粒促進愈閤的機製.方法 製備EGF納米微粒,測定EGF納米微粒載藥率、包封率和釋藥行為.用打孔器製備糖尿病大鼠皮膚創麵模型.分為4組:EGF納米組(給予含1μg/d EGF的納米微粒),EGF組(給予1μg/d EGF),空微粒組(給予不含EGF納米微粒),燐痠鹽緩遲液(PBS)對照組,于3、7、14、21 d照相併取材,計算創麵愈閤率併比較創麵病理學變化及EGF受體暘性細胞麵積.結果 EGF納米微粒平均粒徑為193.5 nm,載藥率為0.02％,包封率為85％.藥物釋放達24h.EGF納米組創麵愈閤比EGF組快[14 d,(93.8±1.8)％比(89.3±2.3)％,P＜0.05;21 d(96.6±1.6)％比(92.1±4.3)％,P＜0.05].EGF納米組創麵內EGF受體暘性細胞麵積高于EGF組[7d,(49.4±6.5)％比(35.1±2.8)％,P ＜0.05;14 d,(80.2±3.8)％比(73.4±1.7)％,P＜0.05].結論 EGF納米微粒上調創麵內細胞EGF受體錶達,加速創麵的愈閤,效果優于EGF.
목적 관찰표피생장인자(EGF)납미미립대당뇨병대서피부창면적유합작용,탐토EGF납미미립촉진유합적궤제.방법 제비EGF납미미립,측정EGF납미미립재약솔、포봉솔화석약행위.용타공기제비당뇨병대서피부창면모형.분위4조:EGF납미조(급여함1μg/d EGF적납미미립),EGF조(급여1μg/d EGF),공미립조(급여불함EGF납미미립),린산염완충액(PBS)대조조,우3、7、14、21 d조상병취재,계산창면유합솔병비교창면병이학변화급EGF수체양성세포면적.결과 EGF납미미립평균립경위193.5 nm,재약솔위0.02％,포봉솔위85％.약물석방체24h.EGF납미조창면유합비EGF조쾌[14 d,(93.8±1.8)％비(89.3±2.3)％,P＜0.05;21 d(96.6±1.6)％비(92.1±4.3)％,P＜0.05].EGF납미조창면내EGF수체양성세포면적고우EGF조[7d,(49.4±6.5)％비(35.1±2.8)％,P ＜0.05;14 d,(80.2±3.8)％비(73.4±1.7)％,P＜0.05].결론 EGF납미미립상조창면내세포EGF수체표체,가속창면적유합,효과우우EGF.
Objective To investigate the effect and mechanism of epidermal growth factor(EGF) nanoparticles on the wounds of diabetic rats.Methods (1) EGF nanoparticles were prepared by the modified multiple emulsion way with poly lactic-co-glycolic acid (PLGA) as carrier.Drug-loaded rate,encapsulation ratio and release of EGF nanoparticles were measured.(2) Diabetic rats model were made with streptozocin (STZ) through vena caudalis.Skin wound model of diabetic rats were prepared by hole puncher and were divided into 4 groups:EGF nanoparticles group (containing 1 μg/d EGF nanoparticles),EGF group(1 μg/d EGF),empty nanometer particle group(without EGF but the quality was equivalent to the EGF nanoparticles/d) and PBS control group.(3) Photographs and sampling at 3,7,14,21 days were taken and wound healing rate were calculated.The positive cells area of epidermal growth factor receptor were compared by immunohistochemistry.Results (1) The average particle diameter of EGF nanoparticles was 193.5 nn.The drug loading ratio was 0.02％ and the encapsulation ratio was 85％.The group of EGF nanoparticles released up to 24 h.(2) Healing of wound of EGF nanoparticles group was fast than that of EGF [14d:(93.8±1.8)％ vs(89.3±2.3)％,P＜0.05;21 d:(96.6±1.6)％ vs(92.1±4.3)％,P＜ 0.05].EGF receptor positive cells area of EGF nanoparticles group was more than that of EGF group [7 d:(49.4±6.5)％ vs(35.1 ±2.8)％,P＜0.05;14 d:(80.2±3.8)％ vs(73.4 ±1.7)％,P＜0.05].Conclusion EGF nanoparticles can upregulate the expression of EGF receptor of cells in wound and accelerate wound healing.The effects are better than that of EGF.
