肥胖%糖尿病,2型%胃旁路术%大鼠
肥胖%糖尿病,2型%胃徬路術%大鼠
비반%당뇨병,2형%위방로술%대서
Obesity%Diabetes mellitus,type 2%Gastric bypass%Rats
目的 研究Roux-en-Y胃旁路术(RYGB)对自发型肥胖糖尿病(ZDF)大鼠模型糖脂代谢及全身炎症水平的影响.方法 30只雄性ZDF大鼠采用完全随机分组方法分为3组:RYGB手术组(n=10)、匹配饮食控制对照组(PF组,n=10)、自由进食对照组(AL组,n=10).定期测量大鼠体重与摄食量.在术前及术后10周时,对各组大鼠行口服糖耐量试验,检测血糖、血脂、肝脏转氨酶及血清肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)与单核细胞趋化蛋白1(MCP-1)等炎症相关指标,处死各组大鼠后取附睾脂肪,采用实时定量-聚合酶链反应(RT-PCR)方法检测脂肪细胞TNF-α、IL-1β与MCP-1 mRNA表达水平.组间数据比较采用t检验与方差分析.结果 术后10周口服糖耐量试验结果表明,RYGB组、PF组及AL组在各个时间点的血糖值比较均有统计学差异[分别为:基线空腹血糖(FPG):(6.8±0.5)、(8.7±2.2)、(20.6±3.0) mmol/L,F=12.4,P＜0.05;30 min:(14.0±0.8)、(19.1±4.3)、(31.7±0.9)mmol/L,F=20.1,P＜0.05;60 min:(15.4±1.1)、(20.2±7.1)、(33.0 ±0.3)mmol/L,F =22.5,P ＜0.05;90 min:(13.2 ±0.6)、(20.0±5.8)、(32.4±0.4)mmol/L,F=14.7,P ＜0.05;120 min:(8.3 ±0.3)、(14.0±5.2)、(29.1±1.2)mmol/L,F =20.5,P ＜0.05].血清总胆固醇(TC)、游离脂肪酸(FFA)水平显著低于PF组和AL组[分别为:TC:(3.82±0.13)比(4.10 ±0.37)比(5.25±0.25) mmol/L,F=15.3,P＜0.05,FFA:(0.47±0.14)比(0.93±0.08)比(0.78 ±0.06) mmol/L,F=3.4,P＜0.05].RYGB组大鼠血清TNF-α、IL-1β水平均较AL组明显下降[分别为TNF-α:(124 ±23)比(532±52)mmol/L,t =5.8,P＜0.05；IL-1β:(61±l6)比(250±32)mmol/L,t=4.3,P＜0.05],且脂肪组织中上述两种炎症因子mRNA在3组大鼠中的表达趋势与血清水平基本一致；而MCP-1在3组中表达无统计学差异(均P＞0.05).结论 Roux-en-Y胃旁路术能显著改善肥胖2型糖尿病大鼠的糖脂代谢,减轻脂肪肝及全身炎症水平,且这种改善作用不依赖于摄食量减少.
目的 研究Roux-en-Y胃徬路術(RYGB)對自髮型肥胖糖尿病(ZDF)大鼠模型糖脂代謝及全身炎癥水平的影響.方法 30隻雄性ZDF大鼠採用完全隨機分組方法分為3組:RYGB手術組(n=10)、匹配飲食控製對照組(PF組,n=10)、自由進食對照組(AL組,n=10).定期測量大鼠體重與攝食量.在術前及術後10週時,對各組大鼠行口服糖耐量試驗,檢測血糖、血脂、肝髒轉氨酶及血清腫瘤壞死因子α(TNF-α)、白細胞介素1β(IL-1β)與單覈細胞趨化蛋白1(MCP-1)等炎癥相關指標,處死各組大鼠後取附睪脂肪,採用實時定量-聚閤酶鏈反應(RT-PCR)方法檢測脂肪細胞TNF-α、IL-1β與MCP-1 mRNA錶達水平.組間數據比較採用t檢驗與方差分析.結果 術後10週口服糖耐量試驗結果錶明,RYGB組、PF組及AL組在各箇時間點的血糖值比較均有統計學差異[分彆為:基線空腹血糖(FPG):(6.8±0.5)、(8.7±2.2)、(20.6±3.0) mmol/L,F=12.4,P＜0.05;30 min:(14.0±0.8)、(19.1±4.3)、(31.7±0.9)mmol/L,F=20.1,P＜0.05;60 min:(15.4±1.1)、(20.2±7.1)、(33.0 ±0.3)mmol/L,F =22.5,P ＜0.05;90 min:(13.2 ±0.6)、(20.0±5.8)、(32.4±0.4)mmol/L,F=14.7,P ＜0.05;120 min:(8.3 ±0.3)、(14.0±5.2)、(29.1±1.2)mmol/L,F =20.5,P ＜0.05].血清總膽固醇(TC)、遊離脂肪痠(FFA)水平顯著低于PF組和AL組[分彆為:TC:(3.82±0.13)比(4.10 ±0.37)比(5.25±0.25) mmol/L,F=15.3,P＜0.05,FFA:(0.47±0.14)比(0.93±0.08)比(0.78 ±0.06) mmol/L,F=3.4,P＜0.05].RYGB組大鼠血清TNF-α、IL-1β水平均較AL組明顯下降[分彆為TNF-α:(124 ±23)比(532±52)mmol/L,t =5.8,P＜0.05；IL-1β:(61±l6)比(250±32)mmol/L,t=4.3,P＜0.05],且脂肪組織中上述兩種炎癥因子mRNA在3組大鼠中的錶達趨勢與血清水平基本一緻；而MCP-1在3組中錶達無統計學差異(均P＞0.05).結論 Roux-en-Y胃徬路術能顯著改善肥胖2型糖尿病大鼠的糖脂代謝,減輕脂肪肝及全身炎癥水平,且這種改善作用不依賴于攝食量減少.
목적 연구Roux-en-Y위방로술(RYGB)대자발형비반당뇨병(ZDF)대서모형당지대사급전신염증수평적영향.방법 30지웅성ZDF대서채용완전수궤분조방법분위3조:RYGB수술조(n=10)、필배음식공제대조조(PF조,n=10)、자유진식대조조(AL조,n=10).정기측량대서체중여섭식량.재술전급술후10주시,대각조대서행구복당내량시험,검측혈당、혈지、간장전안매급혈청종류배사인자α(TNF-α)、백세포개소1β(IL-1β)여단핵세포추화단백1(MCP-1)등염증상관지표,처사각조대서후취부고지방,채용실시정량-취합매련반응(RT-PCR)방법검측지방세포TNF-α、IL-1β여MCP-1 mRNA표체수평.조간수거비교채용t검험여방차분석.결과 술후10주구복당내량시험결과표명,RYGB조、PF조급AL조재각개시간점적혈당치비교균유통계학차이[분별위:기선공복혈당(FPG):(6.8±0.5)、(8.7±2.2)、(20.6±3.0) mmol/L,F=12.4,P＜0.05;30 min:(14.0±0.8)、(19.1±4.3)、(31.7±0.9)mmol/L,F=20.1,P＜0.05;60 min:(15.4±1.1)、(20.2±7.1)、(33.0 ±0.3)mmol/L,F =22.5,P ＜0.05;90 min:(13.2 ±0.6)、(20.0±5.8)、(32.4±0.4)mmol/L,F=14.7,P ＜0.05;120 min:(8.3 ±0.3)、(14.0±5.2)、(29.1±1.2)mmol/L,F =20.5,P ＜0.05].혈청총담고순(TC)、유리지방산(FFA)수평현저저우PF조화AL조[분별위:TC:(3.82±0.13)비(4.10 ±0.37)비(5.25±0.25) mmol/L,F=15.3,P＜0.05,FFA:(0.47±0.14)비(0.93±0.08)비(0.78 ±0.06) mmol/L,F=3.4,P＜0.05].RYGB조대서혈청TNF-α、IL-1β수평균교AL조명현하강[분별위TNF-α:(124 ±23)비(532±52)mmol/L,t =5.8,P＜0.05；IL-1β:(61±l6)비(250±32)mmol/L,t=4.3,P＜0.05],차지방조직중상술량충염증인자mRNA재3조대서중적표체추세여혈청수평기본일치；이MCP-1재3조중표체무통계학차이(균P＞0.05).결론 Roux-en-Y위방로술능현저개선비반2형당뇨병대서적당지대사,감경지방간급전신염증수평,차저충개선작용불의뢰우섭식량감소.
Objective To investigate the effect of Roux-en-Y gastric bypass (RYGB) on glucose and lipid metabolism and systemic inflammation levels in spontaneous Zucker Diabetic Fatty (ZDF) rats.Methods Thirty male ZDF rats were randomly divided to three groups: RYGB surgery group (n =10),sham surgery pair-fed (PF) group (n =10) and sham surgery ad libitum fed (AL) group (n =10).Body weight,food intake was monitored.Oral glucose tolerance test,serum lipid level,liver transaminases,TNF-α,IL-1 β and MCP-1 were measured before and 10-week after surgery.Then,rats were sacrificed.The epididymal fat were taken from all groups of rats.The mRNA expression of TNF-α,IL-1 β and MCP-1 in epididymal fat of rats were detected using RT-PCR method.The data was analyzed by t test and analysis of variance.Result Oral glucose tolerance test showed that blood glucose were significantly different among the RYGB group,AL group and PF group after 10 weeks postoperatively (FPG (6.8 ± 0.5),(8.7 ± 2.2),(20.6 ±3.0) mmol/L,F=12.4,P＜0.05,30 min (14.0 ±0.8),(19.1 ±4.3),(31.7 ±0.9) mmol/L,F=20.1,P＜0.05,60 min (15.4±1.1),(20.2 ±7.1),(33.0±0.3) mmol/L,F=22.5,P＜0.05,90min (13.2 ±0.6),(20.0 ±5.8),(32.4 ±0.4) mmol/L,F =14.7,P ＜0.05,120 min (8.3 ±0.3),(14.0 ± 5.2),(29.1 ± 1.2) mmol/L,F =20.5,P ＜ 0.05).Serum total cholesterol (TC) and free fatty acids (FFA) was decreased in the RYGB group,compared with PF and AL controls (TC (3.82 ± 0.13) vs (4.10±0.37) vs (5.25 ±0.25) mmol/L,F=15.3,FFA (0.47 ±0.14) vs (0.93 ±0.08) vs (0.78 ±0.06) mmol/L,F =3.4,both P ＜ 0.05).And liver transaminases (ALT and AST) levels in RYGB group were lower than AL group,but higher than PF group (ALT (129 ±28) vs (67 ±7) vs (726 ± 160) mmol/L,F =15.1,AST (208±26) vs (124±10) vs (673±209) mmol/L,F=5.9,bothP＜0.05) and the serum levels of TNF-α and IL-1 βin RYGB group also decreased,compared with those in AL group (TNF-α (124 ± 23) vs (532 ± 52) mmol/L,t =5.8 ; IL-1 β (61 ± 16) vs (250 ± 32) mmol/L,t =4.3,both P ＜ 0.05).The mRNA expression of above two factors coincided with the serum levels in three groups.However,MCP-1 expression were similar among the groups (P ＞ 0.05 all above).Conclusion RYGB can significantly moderate glucose homeostasis and lipid metabolism,decrease fatty liver and systemic inflammation levels in the ZDF rat model.This effect is not reliable to the reduction of food intake.
