中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2014年
6期
407-410
,共4页
田骆冰%徐刚%秦江媛%孙雪玲%王爱媛%田金莉%张雅中%许静%李烨
田駱冰%徐剛%秦江媛%孫雪玲%王愛媛%田金莉%張雅中%許靜%李燁
전락빙%서강%진강원%손설령%왕애원%전금리%장아중%허정%리엽
糖尿病,2型%老年人%血管内皮生长因子%下肢血管病变
糖尿病,2型%老年人%血管內皮生長因子%下肢血管病變
당뇨병,2형%노년인%혈관내피생장인자%하지혈관병변
Diabetes mellitus,type 2%Aged%Vascular endothelial growth factor%Peripheral artery disease
目的 探讨老年糖尿病患者血浆血管内皮生长因子(VEGF)水平及外周血单个核细胞(PBMCs) VEGF的mRNA表达与糖尿病下肢血管病变(PAD)的关系.方法 选取2009年1月至2012年1月于唐山市工人医院就诊的40例单纯2型糖尿病未合并下肢血管病变患者(DM组)、46例糖尿病合并间歇性跛行患者(IC组)和40例糖尿病合并临界性肢体缺血患者(CLI组).分别检测3组患者血浆VEGF水平和PBMCs的VEGF的mRNA表达水平,并计算VEGF诊断PAD的切点.组间差异比较应用方差分析x2检验或秩和检验.结果 3组间血浆VEGF水平及PBMCs VEGF mRNA表达差异有统计学意义(F=37.87、36.41,均P＜0.05);且CLI组血浆VEGF水平及PBMC的VEGFmRNA表达均显著高于IC组[(568±201)比(392±144) ng/L;4.47(2.20～9.06)比2.58(1.21～5.50),均P＜0.05];当血浆VEGF≥345.58 ng/L时,诊断PAD的灵敏度和特异度最高.3组中血浆VEGF水平和PBMC的VEGF mRNA表达与踝肱指数(ABI)呈负相关(r=-0.495 ～-0.319,均P＜0.05).IC组和CLI组血浆VEGF水平与PBMC的VEGF mRNA表达呈正相关(r=0.341、0.409,均P＜0.05).结论 VEGF与糖尿病PAD密切相关.
目的 探討老年糖尿病患者血漿血管內皮生長因子(VEGF)水平及外週血單箇覈細胞(PBMCs) VEGF的mRNA錶達與糖尿病下肢血管病變(PAD)的關繫.方法 選取2009年1月至2012年1月于唐山市工人醫院就診的40例單純2型糖尿病未閤併下肢血管病變患者(DM組)、46例糖尿病閤併間歇性跛行患者(IC組)和40例糖尿病閤併臨界性肢體缺血患者(CLI組).分彆檢測3組患者血漿VEGF水平和PBMCs的VEGF的mRNA錶達水平,併計算VEGF診斷PAD的切點.組間差異比較應用方差分析x2檢驗或秩和檢驗.結果 3組間血漿VEGF水平及PBMCs VEGF mRNA錶達差異有統計學意義(F=37.87、36.41,均P＜0.05);且CLI組血漿VEGF水平及PBMC的VEGFmRNA錶達均顯著高于IC組[(568±201)比(392±144) ng/L;4.47(2.20～9.06)比2.58(1.21～5.50),均P＜0.05];噹血漿VEGF≥345.58 ng/L時,診斷PAD的靈敏度和特異度最高.3組中血漿VEGF水平和PBMC的VEGF mRNA錶達與踝肱指數(ABI)呈負相關(r=-0.495 ～-0.319,均P＜0.05).IC組和CLI組血漿VEGF水平與PBMC的VEGF mRNA錶達呈正相關(r=0.341、0.409,均P＜0.05).結論 VEGF與糖尿病PAD密切相關.
목적 탐토노년당뇨병환자혈장혈관내피생장인자(VEGF)수평급외주혈단개핵세포(PBMCs) VEGF적mRNA표체여당뇨병하지혈관병변(PAD)적관계.방법 선취2009년1월지2012년1월우당산시공인의원취진적40례단순2형당뇨병미합병하지혈관병변환자(DM조)、46례당뇨병합병간헐성파행환자(IC조)화40례당뇨병합병림계성지체결혈환자(CLI조).분별검측3조환자혈장VEGF수평화PBMCs적VEGF적mRNA표체수평,병계산VEGF진단PAD적절점.조간차이비교응용방차분석x2검험혹질화검험.결과 3조간혈장VEGF수평급PBMCs VEGF mRNA표체차이유통계학의의(F=37.87、36.41,균P＜0.05);차CLI조혈장VEGF수평급PBMC적VEGFmRNA표체균현저고우IC조[(568±201)비(392±144) ng/L;4.47(2.20～9.06)비2.58(1.21～5.50),균P＜0.05];당혈장VEGF≥345.58 ng/L시,진단PAD적령민도화특이도최고.3조중혈장VEGF수평화PBMC적VEGF mRNA표체여과굉지수(ABI)정부상관(r=-0.495 ～-0.319,균P＜0.05).IC조화CLI조혈장VEGF수평여PBMC적VEGF mRNA표체정정상관(r=0.341、0.409,균P＜0.05).결론 VEGF여당뇨병PAD밀절상관.
Objective To investigate plamsa levels of vascular endothelial growth factor(VEGF) and its mRNA expression levels in peripheral blood mononuclear cells (PBMCs) from type 2 diabetes mellitus (T2DM) patients with peripheral artery disease (PAD) and their correlation with PAD among T2DM patients.Methods Total of 126 T2DM patients were enrolled from January 2009 to January 2012 in Tangshan Gongren Hospital in this study; and the patients were divided into three groups including T2DM patients without PAD (DM group,n =40),T2DM with intermittent claudication (IC group,n =46),and T2DM with critical limb ischemia (CLI group,n =40).Plasma level of VEGF and its expression level in PBMCs in the three groups were detected by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction(RT-PCR),respectively,and the cutoff value of VEGF to diagnose T2DM with PAD was evaluated.Statistical analysis were performed by using one-way analysis of variance,x2 test,and nonparameter test for multiple-group comparisons.Results There was significant difference in plasma levels of VEGF and its mRNA levels in PBMCs among the three groups (F =37.87,36.41,both P＜0.05).Moreover,both plasma levels of VEGF and its mRNA levels in PBMCs were significantly increased in CLI group compared with those in IC group [(568 ±201) vs (392 ± 144) ng/L,4.47(2.20-9.06) vs 2.58 (1.21-5.50),both P ＜0.05].The VEGF plasma level ≥345.58 ng/L for the diagnosis of T2DM with PAD was the cutoff value with the highest sensitivity and specificity.Plasma levels of VEGF and its mRNA levels in PMBCs were negatively correlated with ankle brachial index among DM group,IC group and CLI group (r =-0.495--0.319,all P ＜ 0.05).Conclusion VEGF is closely associated with PAD in diabetics.
