CAJ | 학술논문

目的研究糖基化终末产物(AGEs)对人胰岛微血管内皮细胞(HIMVEC)黏附分子表达和白细胞黏附的影响.方法 HIMVEC细胞在200 mg/L的AGEs刺激后,用细胞基础的酶联免疫吸附法(ELISA)和Western blotting法检测细胞表面黏附分子的表达;并与BCECF标记的白细胞共培养检测与白细胞的黏附能力.采用实时荧光定量聚合酶链反应(RT-PCR)和Western blotting分别检测HIMVEC细胞上AGEs受体(RAGE)、蛋白激酶Cβ(PKC β)和蛋白激酶A(PKA)的mRNA表达情况.随后给予PKC β抑制剂LY333531或PKA激活剂8-Br-cAMP,观察对HIMVEC黏附分子表达的影响及和白细胞黏附的影响.两组间比较采用t检验进行分析.结果与对照组相比,AGEs处理后HIMVEC表达P选择素、E选择素和血管细胞黏附分子1(VCAM-1)均上调(分别为1.10 ±0.13比0.64±0.14,0.83 ±0.06比0.47 ±0.05,0.87 ±0.09比0.43±0.07,t =4.93、9.40、7.61,均P＜0.05),并且与白细胞的黏附较对照组明显增加(54 ±4比23 ±3,t=12.69,P＜0.05).与AGEs组比较,RAGE抗体组P选择素、E选择素和VCAM-1的表达明显降低,组间差异具有统计学意义(t=5.69、6.89、5.43,均P＜0.05).RAGE抗体组白细胞黏附明显少于AGEs组(54±4比31 ±4,t=8.22,P＜0.05).与对照组相比,AGEs处理HIMVEC 4 h和18h,在mRNA水平和蛋白质水平均检测到RAGE和PKCβ的表达上调,但PKA的表达下调(t=10.94、7.76、21.82、5.85、10.96、11.47,均P＜0.05).与AGEs组相比,在AGEs处理时给予PKCβ抑制剂LY333531或PKA激活剂8-Br-cAMP,均可降低HIMVEC上P选择素、E选择素和VCAM-1的表达水平(=7.60、6.60、6.25、11.58、4.08、3.47,均P＜0.05),并减少白细胞的黏附(t=7.67、8.89,均P＜0.05).结论 AGEs通过RAGE受体上调PKCβ和下调PKA增加HIMVEC上黏附分子的表达,促进白细胞的黏附,可能是糖尿病状态下胰岛中白细胞浸润的机制之一.
목적 연구당기화종말산물(AGEs)대인이도미혈관내피세포(HIMVEC)점부분자표체화백세포점부적영향.방법 HIMVEC세포재200 mg/L적AGEs자격후,용세포기출적매련면역흡부법(ELISA)화Western blotting법검측세포표면점부분자적표체;병여BCECF표기적백세포공배양검측여백세포적점부능력.채용실시형광정량취합매련반응(RT-PCR)화Western blotting분별검측HIMVEC세포상AGEs수체(RAGE)、단백격매Cβ(PKC β)화단백격매A(PKA)적mRNA표체정황.수후급여PKC β억제제LY333531혹PKA격활제8-Br-cAMP,관찰대HIMVEC점부분자표체적영향급화백세포점부적영향.량조간비교채용t검험진행분석.결과 여대조조상비,AGEs처리후HIMVEC표체P선택소、E선택소화혈관세포점부분자1(VCAM-1)균상조(분별위1.10 ±0.13비0.64±0.14,0.83 ±0.06비0.47 ±0.05,0.87 ±0.09비0.43±0.07,t =4.93、9.40、7.61,균P＜0.05),병차여백세포적점부교대조조명현증가(54 ±4비23 ±3,t=12.69,P＜0.05).여AGEs조비교,RAGE항체조P선택소、E선택소화VCAM-1적표체명현강저,조간차이구유통계학의의(t=5.69、6.89、5.43,균P＜0.05).RAGE항체조백세포점부명현소우AGEs조(54±4비31 ±4,t=8.22,P＜0.05).여대조조상비,AGEs처리HIMVEC 4 h화18h,재mRNA수평화단백질수평균검측도RAGE화PKCβ적표체상조,단PKA적표체하조(t=10.94、7.76、21.82、5.85、10.96、11.47,균P＜0.05).여AGEs조상비,재AGEs처리시급여PKCβ억제제LY333531혹PKA격활제8-Br-cAMP,균가강저HIMVEC상P선택소、E선택소화VCAM-1적표체수평(=7.60、6.60、6.25、11.58、4.08、3.47,균P＜0.05),병감소백세포적점부(t=7.67、8.89,균P＜0.05).결론 AGEs통과RAGE수체상조PKCβ화하조PKA증가HIMVEC상점부분자적표체,촉진백세포적점부,가능시당뇨병상태하이도중백세포침윤적궤제지일.
Objective To investigate the effect of advanced glycation end products (AGEs) on adhesion molecules expression and leukocyte adhesion on human islet microvascular endothelial cells (HIMVEC),and the related mechanisms.Methods HIMVEC was treated with AGEs (final concentration 200 mg/L).Cell-based enzyme-linked immunosorbent assay (ELISA) and western blotting were used to detect adhesion molecules level of HIMVEC; the leukocyte-HIMVEC adhesion was evaluated after BCECF-labeled human leukocytes incubated with HIMVEC.The expression levels of receptor of AGEs (RAGE),protein kinase C β (PKCβ) and protein kinase A (PKA) were detected by real-time PCR and Western blotting.The adhesion molecules expression and leukocyte adhesion were evaluated after cells treated with PKCβ inhibitor (LY333531) or PKA activator (8-Br-cAMP).t-test were used to compare the difference between groups for statistical analysis.Results Compared to control group,P-selectin,E-selectin and Vascular cell adhesion molecule-1 (VCAM-1) were all up-regulated on HIMVEC by AGEs treatment (1.1 ± 0.13 vs 0.64 ±0.14,0.83 ±0.06 vs 0.47 ±0.05,0.87 ±0.09 vs 0.43 ±0.07,t =4.93,9.40,7.61,all P ＜0.05),and leukocytes adhesion was increased in AGEs group than control (54 ± 4 vs 23 ± 3,t =12.69,P ＜0.05).Compared to AGEs group,the expression levels of P-selectin,E-selectin and VCAM-1 were decreased by pre-treated with anti-RAGE antibody (t =5.69,6.89,5.43,all P ＜ 0.05),and leukocyte adhesion was also inhibited by RAGE antibody (54 ± 4 vs 31 ± 4,t =8.22,P ＜ 0.05).Compared to control group,after treated with AGEs for 4 h or 18 h,the mRNA and protein level of RAGE and PKCβ was up-regulated,but PKA was down-regulated (t =10.94,7.76,21.82,5.85,10.96,11.47,all P ＜ 0.05).Compared to AGEs group,PKCβ inhibitor (LY333531) or PKA activator (8-Br-cAMP) can down-regulate the expression of P-selectin,E-selectin and VCAM-1 (t =7.60,6.60,6.25,11.58,4.08,3.47,all P ＜ 0.05).And leukocyte adhesion was also reduced in LY333531 or 8-Br-cAMP group than AGEs group (t =7.67,8.89,both P ＜ 0.05).Conclusion AGEs increased adhesion molecules expression and leukocyte adhesion on HIMVEC by up-regulating PKCB and down-regulating PKA,which might be one reason for leukocyte infiltration in islets of type 2 diabetes.