中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2014年
8期
579-583
,共5页
刘红%刘行海%买文丽%刘华%曹弟勇%兰海涛%霍雯%郑倩
劉紅%劉行海%買文麗%劉華%曹弟勇%蘭海濤%霍雯%鄭倩
류홍%류행해%매문려%류화%조제용%란해도%곽문%정천
糖尿病,2型%内质网%胰岛细胞%凋亡细胞%虫草菌丝
糖尿病,2型%內質網%胰島細胞%凋亡細胞%蟲草菌絲
당뇨병,2형%내질망%이도세포%조망세포%충초균사
Diabetes mellitus,type 2%Endoplasmic reticulum%Islet cell%Apoptosis%Cordyceps sinensis
目的 观察虫草菌丝(cordyceps sinensis,CS)对2型糖尿病(T2DM)大鼠胰岛细胞的保护作用及机制.方法 采用链脲佐菌素联合高脂饮食建立T2DM大鼠模型,成模后大鼠分为T2DM组、虫草菌丝低剂量组(2ml·kg-1·d-1,CS1组)、虫草菌丝高剂量组(5ml·kg-1·d-1,CS2组),同时设置正常对照组.CS1组、CS2组用CS干预12周.观察CS对大鼠糖代谢的影响,进行各组大鼠空腹血糖(FBG)、空腹胰岛素水平(FINS)的比较,并计算胰岛素敏感指数(ISI).胰岛细胞中凋亡的变化采用TUNEL方法测定,测定胰岛内质网应激蛋白(CHOP、JNK)的表达,其表达水平采用免疫组化进行检测.本实验多个样本均数间比较,采用F检验,组间两两比较采用SNK-q法检验,同一组干预前后比较用t检验.结果 (1)FBG、FINS和ISI变化:CS干预12周后,CS1、CS2组FBG [(15.8±2.6)、(13.1±2.3)mmol/L]低于T2DM组[(20.5±3.7)mmol/L; q=8.56、10.20,均P＜0.01];CS1、CS2组FINS[(19.7±3.3)、(15.5±1.9) mU/L]低于T2DM组[(22.3±3.7)mU/L; q=5.28、7.44,均P＜0.01];CS1、CS2组ISI [(-5.43±0.32)、(-4.35±0.24)]高于T2DM组[(-5.94±0.41);q=6.38、8.52,均P＜0.01].(2)大鼠胰岛凋亡指数:T2DM组显著高于正常对照组[(0.583±0.137)比(0.154±0.042); q=12.02,P＜0.01]和CS1、CS2组[(0.369±0.032)、(0.241±0.024);q=4.58、9.87,P＜0.05和P＜0.01].(3)大鼠胰岛CHOP、JNK的表达:T2DM组[(0.362±0.064)、(1.35±0.15)]高于正常对照组[(0.063±0.021)、(0.12±0.03);q=18.87、26.28,均P＜0.01]、CS1组[(0.268±0.038)、(0.56±0.07); q=4.26、6.75,P＜0.05,P＜0.01]和CS2组[(0.152±0.035)、(0.33±0.06); q=7.84、8.97,均P＜0.01].结论 CS能够保护T2DM大鼠胰岛细胞的功能,其机制可能与减轻胰岛细胞凋亡的发生,抑制胰岛内质网应激水平,降低CHOP、JUN的表达有关.
目的 觀察蟲草菌絲(cordyceps sinensis,CS)對2型糖尿病(T2DM)大鼠胰島細胞的保護作用及機製.方法 採用鏈脲佐菌素聯閤高脂飲食建立T2DM大鼠模型,成模後大鼠分為T2DM組、蟲草菌絲低劑量組(2ml·kg-1·d-1,CS1組)、蟲草菌絲高劑量組(5ml·kg-1·d-1,CS2組),同時設置正常對照組.CS1組、CS2組用CS榦預12週.觀察CS對大鼠糖代謝的影響,進行各組大鼠空腹血糖(FBG)、空腹胰島素水平(FINS)的比較,併計算胰島素敏感指數(ISI).胰島細胞中凋亡的變化採用TUNEL方法測定,測定胰島內質網應激蛋白(CHOP、JNK)的錶達,其錶達水平採用免疫組化進行檢測.本實驗多箇樣本均數間比較,採用F檢驗,組間兩兩比較採用SNK-q法檢驗,同一組榦預前後比較用t檢驗.結果 (1)FBG、FINS和ISI變化:CS榦預12週後,CS1、CS2組FBG [(15.8±2.6)、(13.1±2.3)mmol/L]低于T2DM組[(20.5±3.7)mmol/L; q=8.56、10.20,均P＜0.01];CS1、CS2組FINS[(19.7±3.3)、(15.5±1.9) mU/L]低于T2DM組[(22.3±3.7)mU/L; q=5.28、7.44,均P＜0.01];CS1、CS2組ISI [(-5.43±0.32)、(-4.35±0.24)]高于T2DM組[(-5.94±0.41);q=6.38、8.52,均P＜0.01].(2)大鼠胰島凋亡指數:T2DM組顯著高于正常對照組[(0.583±0.137)比(0.154±0.042); q=12.02,P＜0.01]和CS1、CS2組[(0.369±0.032)、(0.241±0.024);q=4.58、9.87,P＜0.05和P＜0.01].(3)大鼠胰島CHOP、JNK的錶達:T2DM組[(0.362±0.064)、(1.35±0.15)]高于正常對照組[(0.063±0.021)、(0.12±0.03);q=18.87、26.28,均P＜0.01]、CS1組[(0.268±0.038)、(0.56±0.07); q=4.26、6.75,P＜0.05,P＜0.01]和CS2組[(0.152±0.035)、(0.33±0.06); q=7.84、8.97,均P＜0.01].結論 CS能夠保護T2DM大鼠胰島細胞的功能,其機製可能與減輕胰島細胞凋亡的髮生,抑製胰島內質網應激水平,降低CHOP、JUN的錶達有關.
목적 관찰충초균사(cordyceps sinensis,CS)대2형당뇨병(T2DM)대서이도세포적보호작용급궤제.방법 채용련뇨좌균소연합고지음식건립T2DM대서모형,성모후대서분위T2DM조、충초균사저제량조(2ml·kg-1·d-1,CS1조)、충초균사고제량조(5ml·kg-1·d-1,CS2조),동시설치정상대조조.CS1조、CS2조용CS간예12주.관찰CS대대서당대사적영향,진행각조대서공복혈당(FBG)、공복이도소수평(FINS)적비교,병계산이도소민감지수(ISI).이도세포중조망적변화채용TUNEL방법측정,측정이도내질망응격단백(CHOP、JNK)적표체,기표체수평채용면역조화진행검측.본실험다개양본균수간비교,채용F검험,조간량량비교채용SNK-q법검험,동일조간예전후비교용t검험.결과 (1)FBG、FINS화ISI변화:CS간예12주후,CS1、CS2조FBG [(15.8±2.6)、(13.1±2.3)mmol/L]저우T2DM조[(20.5±3.7)mmol/L; q=8.56、10.20,균P＜0.01];CS1、CS2조FINS[(19.7±3.3)、(15.5±1.9) mU/L]저우T2DM조[(22.3±3.7)mU/L; q=5.28、7.44,균P＜0.01];CS1、CS2조ISI [(-5.43±0.32)、(-4.35±0.24)]고우T2DM조[(-5.94±0.41);q=6.38、8.52,균P＜0.01].(2)대서이도조망지수:T2DM조현저고우정상대조조[(0.583±0.137)비(0.154±0.042); q=12.02,P＜0.01]화CS1、CS2조[(0.369±0.032)、(0.241±0.024);q=4.58、9.87,P＜0.05화P＜0.01].(3)대서이도CHOP、JNK적표체:T2DM조[(0.362±0.064)、(1.35±0.15)]고우정상대조조[(0.063±0.021)、(0.12±0.03);q=18.87、26.28,균P＜0.01]、CS1조[(0.268±0.038)、(0.56±0.07); q=4.26、6.75,P＜0.05,P＜0.01]화CS2조[(0.152±0.035)、(0.33±0.06); q=7.84、8.97,균P＜0.01].결론 CS능구보호T2DM대서이도세포적공능,기궤제가능여감경이도세포조망적발생,억제이도내질망응격수평,강저CHOP、JUN적표체유관.
Objective To investigate the protective effects and mechanism of cordyceps sinensis (CS) on the islets cells of rats with type 2 diabetes mellitus.Methods Streptozotocin combined with high-fat diet was used to induce T2DM rats' model.The rats were randomly divided into three groups:model control group (MOR),low dose group (CS1,2 ml·kg-1·d-1,i.p.) and high dose group (CS2,5 ml·kg-1·d-1,i.p.).Two treatment groups (CS1 and CS2) were given CS for 12 weeks and a normal control group (NOR) was set to observe the effects of CS on rats' glucose metabolism.The following parameters were measured:the fasting blood glucose (FBG),the fasting insulin level (FINS) and the insulin sensitivity index (ISI).TUNEL was used to detect the islets apoptosis.Immunohistochemistry was used to assess the expression level of islet CHOP and JNK stress proteins.Results (1)Rats were given CS for 12 weeks,FBG of CS1 and CS2 group,respectively (15.8±2.6),(13.1±2.3) mmol/L,were obviously lower than that of MOR group (20.5±3.7) mmol/L,with q values respectively 8.56,10.20,P values all below 0.01.ISI of CS1 and CS2 group,respectively (-5.43±0.32),(-4.35±0.24),were obviously higher than that of NOR group (-5.94±0.41),with q values:6.38,8.52,P＜0.01.(2)AI of MOR group (0.583±0.137) was obviously higher than that of NOR group (0.154±0.042),(q=12.02,P＜0.01).AI of CS1 and CS2,respectively (0.369±0.032),(0.241 ±0.024),were obviously lower than that of MOR,with q values:4.58,9.87,P values below 0.05,0.01.(3)The expression levels of CHOP and JNK in MOR group,respectively (0.362±0.064),(1.35 ± 0.15),were obviously higher than those of NOR group,respectively (0.063±0.021),(0.12±0.03),with q values:18.87,26.28,P＜0.01.The expression levels of CHOP and JNK in CS1 were (0.268±0.038) and (0.56±0.07),obviously lower than those of MOR group,with q values:4.26,6.75,P values below 0.05,0.01.CHOP and JNK expression levels of CS2.respectively (0.152± 0.035) and (0.33±0.06),were obviously lower than those of MOR group,with q values:7.84,8.97 (P＜0.01).Conclusion CS can protect the function of T2DM rats' islet cells; the mechanism may relate to reducing apoptosis,decreasing the level of ERS and the expression level of CHOP and JUN.
