中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2012年
12期
732-736
,共5页
张俊农%许群星%韩玉环%张立军%华绍芳%董微
張俊農%許群星%韓玉環%張立軍%華紹芳%董微
장준농%허군성%한옥배%장립군%화소방%동미
高血压,妊娠性%胎盘%子宫肌层%转化生长因子β1%抗原,CD%受体,细胞表面
高血壓,妊娠性%胎盤%子宮肌層%轉化生長因子β1%抗原,CD%受體,細胞錶麵
고혈압,임신성%태반%자궁기층%전화생장인자β1%항원,CD%수체,세포표면
Hypertension,pregnancy-induced%Placenta%Myometrium%Transforming growth factor beta 1%Antigens,CD%Receptors,cell surface
目的 研究妊娠期高血压和子痫前期患者胎盘组织和子宫浅肌层中转化生长因子-β1(transforming growth factor-β1,TGF-β1)、endoglin(Eng)蛋白及mRNA表达水平及其相关性,探讨Eng与TGF-β1在妊娠期高血压和子痫前期发病中的作用. 方法 选取2009年4月至2010年4月在天津医科大学第二医院产科住院并分娩的孕妇共110例,其中妊娠期高血压组30例,轻度子痫前期组30例,重度子痫前期组30例,正常对照组20例.剖宫产时取胎盘及子宫浅肌层组织.采用Western印迹技术测定胎盘及子宫浅肌层中Eng、TGF-β1蛋白的表达；实时荧光定量聚合酶链反应技术检测各组孕妇胎盘及子宫浅肌层中Eng及TGF-β1 mRNA表达情况.多组比较采用单因素方差分析,两两比较采用Student-Newman-Keuls检验；相关性用Pearson相关分析及直线回归分析.结果 在正常对照组、妊娠期高血压组、轻度子痫前期组和重度子痫前期组,胎盘组织Eng蛋白表达水平分别为0.11±0.07、0.15±0.05、0.18±0.06和0.43±0.04,TGF-β1蛋白表达水平分别为0.11±0.02、0.26±0.05、0.27=0.03和0.88±0.09;子宫浅肌层中Eng蛋白表达水平分别为0.14±0.06、0.16±0.04、0.20±0.08和0.46±0.05,TGF-β1蛋白表达水平分别为0.15±0.03、0.29±0.06、0.31±0.04和0.91±0.08.在正常对照组、妊娠期高血压组、轻度子痫前期组和重度子痫前期组,胎盘组织Eng mRNA表达水平分别为1.00、1.27±0.58、1.54±0.41和1.83±0.35,TGF-β1 mRNA表达水平分别为1.00、1.64±0.33、1.92±0.38和2.23±0.53;子宫浅肌层中Eng mRNA表达水平分别为1.00、1.32±0.46、1.59±0.37和1.93±0.52,TGF-β1 mRNA表达水平分别为1.00、1.71±0.45、1.91±0.51和2.37±0.46.随着病情的加重,2种因子蛋白和mRNA的表达均呈上升趋势,组间差异均有统计学意义(P均＜0.05).妊娠期高血压组、轻度子痫前期组及重度子痫前期组孕妇胎盘中TGF-β1与Eng蛋白水平呈正相关(r值分别为0.57、0.61和0.60,P＜0.05)；在子宫浅肌层中表达亦呈正相关(r值分别为0.59、0.62和0.61,P＜0.05). 结论 Eng及TGF-β1可能参与了妊娠期高血压和子痫前期的发生和发展.
目的 研究妊娠期高血壓和子癇前期患者胎盤組織和子宮淺肌層中轉化生長因子-β1(transforming growth factor-β1,TGF-β1)、endoglin(Eng)蛋白及mRNA錶達水平及其相關性,探討Eng與TGF-β1在妊娠期高血壓和子癇前期髮病中的作用. 方法 選取2009年4月至2010年4月在天津醫科大學第二醫院產科住院併分娩的孕婦共110例,其中妊娠期高血壓組30例,輕度子癇前期組30例,重度子癇前期組30例,正常對照組20例.剖宮產時取胎盤及子宮淺肌層組織.採用Western印跡技術測定胎盤及子宮淺肌層中Eng、TGF-β1蛋白的錶達；實時熒光定量聚閤酶鏈反應技術檢測各組孕婦胎盤及子宮淺肌層中Eng及TGF-β1 mRNA錶達情況.多組比較採用單因素方差分析,兩兩比較採用Student-Newman-Keuls檢驗；相關性用Pearson相關分析及直線迴歸分析.結果 在正常對照組、妊娠期高血壓組、輕度子癇前期組和重度子癇前期組,胎盤組織Eng蛋白錶達水平分彆為0.11±0.07、0.15±0.05、0.18±0.06和0.43±0.04,TGF-β1蛋白錶達水平分彆為0.11±0.02、0.26±0.05、0.27=0.03和0.88±0.09;子宮淺肌層中Eng蛋白錶達水平分彆為0.14±0.06、0.16±0.04、0.20±0.08和0.46±0.05,TGF-β1蛋白錶達水平分彆為0.15±0.03、0.29±0.06、0.31±0.04和0.91±0.08.在正常對照組、妊娠期高血壓組、輕度子癇前期組和重度子癇前期組,胎盤組織Eng mRNA錶達水平分彆為1.00、1.27±0.58、1.54±0.41和1.83±0.35,TGF-β1 mRNA錶達水平分彆為1.00、1.64±0.33、1.92±0.38和2.23±0.53;子宮淺肌層中Eng mRNA錶達水平分彆為1.00、1.32±0.46、1.59±0.37和1.93±0.52,TGF-β1 mRNA錶達水平分彆為1.00、1.71±0.45、1.91±0.51和2.37±0.46.隨著病情的加重,2種因子蛋白和mRNA的錶達均呈上升趨勢,組間差異均有統計學意義(P均＜0.05).妊娠期高血壓組、輕度子癇前期組及重度子癇前期組孕婦胎盤中TGF-β1與Eng蛋白水平呈正相關(r值分彆為0.57、0.61和0.60,P＜0.05)；在子宮淺肌層中錶達亦呈正相關(r值分彆為0.59、0.62和0.61,P＜0.05). 結論 Eng及TGF-β1可能參與瞭妊娠期高血壓和子癇前期的髮生和髮展.
목적 연구임신기고혈압화자간전기환자태반조직화자궁천기층중전화생장인자-β1(transforming growth factor-β1,TGF-β1)、endoglin(Eng)단백급mRNA표체수평급기상관성,탐토Eng여TGF-β1재임신기고혈압화자간전기발병중적작용. 방법 선취2009년4월지2010년4월재천진의과대학제이의원산과주원병분면적잉부공110례,기중임신기고혈압조30례,경도자간전기조30례,중도자간전기조30례,정상대조조20례.부궁산시취태반급자궁천기층조직.채용Western인적기술측정태반급자궁천기층중Eng、TGF-β1단백적표체；실시형광정량취합매련반응기술검측각조잉부태반급자궁천기층중Eng급TGF-β1 mRNA표체정황.다조비교채용단인소방차분석,량량비교채용Student-Newman-Keuls검험；상관성용Pearson상관분석급직선회귀분석.결과 재정상대조조、임신기고혈압조、경도자간전기조화중도자간전기조,태반조직Eng단백표체수평분별위0.11±0.07、0.15±0.05、0.18±0.06화0.43±0.04,TGF-β1단백표체수평분별위0.11±0.02、0.26±0.05、0.27=0.03화0.88±0.09;자궁천기층중Eng단백표체수평분별위0.14±0.06、0.16±0.04、0.20±0.08화0.46±0.05,TGF-β1단백표체수평분별위0.15±0.03、0.29±0.06、0.31±0.04화0.91±0.08.재정상대조조、임신기고혈압조、경도자간전기조화중도자간전기조,태반조직Eng mRNA표체수평분별위1.00、1.27±0.58、1.54±0.41화1.83±0.35,TGF-β1 mRNA표체수평분별위1.00、1.64±0.33、1.92±0.38화2.23±0.53;자궁천기층중Eng mRNA표체수평분별위1.00、1.32±0.46、1.59±0.37화1.93±0.52,TGF-β1 mRNA표체수평분별위1.00、1.71±0.45、1.91±0.51화2.37±0.46.수착병정적가중,2충인자단백화mRNA적표체균정상승추세,조간차이균유통계학의의(P균＜0.05).임신기고혈압조、경도자간전기조급중도자간전기조잉부태반중TGF-β1여Eng단백수평정정상관(r치분별위0.57、0.61화0.60,P＜0.05)；재자궁천기층중표체역정정상관(r치분별위0.59、0.62화0.61,P＜0.05). 결론 Eng급TGF-β1가능삼여료임신기고혈압화자간전기적발생화발전.
Objective To investigate the expression of transforming growth factor-β1 (TGF-β1),endoglin (Eng) and their mRNA in placenta and superficial myometrium of patients with gestational hypertension or preeclampsia and to explore the role of Eng and TGF-β1 in the pathogenesis of gestational hypertension or preeclampsia.Methods One hundred and ten pregnant women were selected in the Second Affiliated Hospital of Tianjin Medical University from April 2009 to April 2010 who underwent cesarean sections and were divided into four groups:gestational hypertension group (n=30),mild preeclampsia group (n=30),severe preeclampsia group (n=30) and control group (normal pregnant women without labor and perinatal complications,n=20).The tissues of placenta and superficial myometrium were collected during cesarean section.Protein levels of Eng and TGF-β1 were detected by Western Blot.Real-time fluorescence reverse transcription polymerase chain reaction was used to detect the Eng and TGF-β1 mRNA expression.One-way ANOVA was used to compare among groups,Student-Newman-Keuls test was used to compare the differences between groups; relation between groups was analyzed by Pearson relation and linear regression.Results In control group,gestational hypertension group,mild and severe preeclampsia group,the Eng mRNA level was 1.00,1.27±0.58,1.54±0.41 and 1.83±0.35,and the TGF-β1 mRNA level was 1.00,1.64 ± 0.33,1.92± 0.38 and 2.23 ± 0.53 in placenta respectively; those figures changed to 1.00,1.32±0.46,1.59±0.37 and 1.93±0.52,and 1.00,1.71 ± 0.45,1.91 ± 0.51 and 2.37 ± 0.46 in superficial myometrium respectively.The Eng and TGF-β1 mRNA levels of control group were lower than those of the other three groups (P＜0.05).The higher the mRNA level,the more severe the disease (P＜ 0.05).In control group,gestational hypertension group,mild and severe preeclampsia group,the Eng protein expression was 0.11±0.07,0.15± 0.05,0.18 ± 0.06 and 0.43 ± 0.04,and the TGF-β1 protein expression was 0.11 ±0.02,0.26 ± 0.05,0.27± 0.03 and 0.88 ± 0.09 in placenta respectively; those figures changed to 0.14±0.06,0.16±0.04,0.20±0.08 and 0.46±0.05,and 0.15±0.03,0.29±0.06,0.31±0.04 and 0.91 ±0.08 in superficial myometrium respectively.The Eng and TGF-β1 protein levels of control group were lower than those of the other three groups (P＜0.05).The higher the protein level,the more severe the disease (P＜0.05).In the gestational hypertension group,mild and severe preeclampsia group,there were positive correlations between Eng and TGF-β1 protein levels in placenta (r=0.57,0.61 and 0.60 respectively,P＜0.05) and superficial myometrium (r=0.59,0.62 and 0.61 respectively,P ＜ 0.05).Conclusions Eng and TGF-β1 might play a role in pathogenesis of gestational hypertension and preeclampsia.
