CAJ | 학술논문

目的了解水通道蛋白3(aquaporin3,AQP3)在小鼠早期胚胎发育中的表达分布情况.方法建立昆明小鼠控制性超排卵模型,收集小鼠四细胞期、八细胞期、桑葚期胚胎及早期囊胚,采用免疫荧光显微镜和激光共聚焦显微镜对AQP3在小鼠早期胚胎中的表达模式及亚细胞定位进行检测分析.结果在小鼠早期胚胎的4个时期中均能检测到特异性AQP3荧光信号,激光共聚焦显微镜观察到AQP3的分布部位在各时期不完全相同,在四细胞期胚胎及八细胞期胚胎主要定位于卵裂球核膜,桑葚期胚胎主要定位于细胞膜,而早期囊胚主要定位于滋养层细胞膜及细胞质.结论 AQP3对小鼠早期胚胎的发育可能具有潜在的调控作用.
목적 료해수통도단백3(aquaporin3,AQP3)재소서조기배태발육중적표체분포정황.방법 건립곤명소서공제성초배란모형,수집소서사세포기、팔세포기、상심기배태급조기낭배,채용면역형광현미경화격광공취초현미경대AQP3재소서조기배태중적표체모식급아세포정위진행검측분석.결과 재소서조기배태적4개시기중균능검측도특이성AQP3형광신호,격광공취초현미경관찰도AQP3적분포부위재각시기불완전상동,재사세포기배태급팔세포기배태주요정위우란렬구핵막,상심기배태주요정위우세포막,이조기낭배주요정위우자양층세포막급세포질.결론 AQP3대소서조기배태적발육가능구유잠재적조공작용.
Objective To investigate the expression and distribution of aquaporin 3 (AQP3) in mouse early embryos at different stages.Methods Controlled ovarian hyperstimulation model of Kunming mouse was used to collect four-cell embryos,eight-cell embryos,morula stage,and early blastocysts.Immunofluorescence microscopy and laser confocal microscopy were used to detect expression and distribution of AQP3 channels in these stages.Results Fluorescence signal of AQP3 was found in four embryonic stages of mice.Distribution within embryo was different at different embryonic stages.AQP3 was mainly expressed on the karyotheca of blastomeres at four-cell and eight-cell stage.In morula stage,AQP3 was mainly expressed on cell membrane of each blastomere.In early blastocysts,AQP3 was predominantly expressed on the cell membrane and cytoplasm of trophoblastic cell.Conclusions AQP3 trans-membrane channel might have potential regulation function on mouse embryonic development.