CAJ | 학술논문

目的观察高压氧(HBO)对急性高原肺水肿(HAPE)模型大鼠水通道蛋白1(AQP1)和水通道蛋白5(AQP5)的影响.方法 56只Wistar大鼠按随机数字法分为正常对照组、高原肺水肿组(HAPE组)、高压氧治疗1次组(1HBOT组)、高压氧治疗2次组(2HBOT组)和常压氧治疗组(NOT组).正常对照组大鼠8只,其余每组12只大鼠.除正常对照组外,余各组制作HAPE模型后分别给予HBO干预.使用Western Blot定量检测各组大鼠肺组织AQP1、AQP5蛋白水平的表达,同时观察肺组织病理变化.结果正常对照组的损伤评分为0,HAPE组、1 HBOT组、2HBOT组和NOT组的损伤评分分别为(4.46±1.54)、(3.17±1.83)、(1.30 ±0.73)和(3.29±2.27)分；HAPE组较其它各组肺组织损伤程度严重,与正常对照组和2HBOT组比较,差异均有统计学意义(P＜0.01)；2HBOT组与NOT组和1HBOT组比较,肺组织损伤程度减轻(P＜0.05).正常对照组AQP1和AQP5的灰度值为1.00,HAPE组、1HBOT组、2HBOT组和NOT组AQP1的相对灰度值分别为(0.58 ±0.20)、(0.76±0.16)、(0.87±0.11)和(0.75±0.27),其AQP5的相对灰度值分别为(0.75±0.12)、(0.80±0.14)、(0.96 ±-0.14)和(0.84±0.18)；HAPE组AQP1和AQP5蛋白水平均较其它各组低,且与正常对照组和2HBOT组间的差异有统计学意义(P＜0.01和P＜0.05)；2HBOT组AQP1和AQP5蛋白水平与1HBOT组比较,均有提高,且2组间比较,AQP5蛋白水平差异有统计学意义(P＜0.05).结论 HAPE模型大鼠肺组织内AQP1、AQP5蛋白水平表达降低；HBO能够上调AQP1、AQP5的表达,减轻肺水肿；对于减轻肺水肿,行2次HBO治疗比行1次HBO治疗及NOT效果更明显.
목적 관찰고압양(HBO)대급성고원폐수종(HAPE)모형대서수통도단백1(AQP1)화수통도단백5(AQP5)적영향.방법 56지Wistar대서안수궤수자법분위정상대조조、고원폐수종조(HAPE조)、고압양치료1차조(1HBOT조)、고압양치료2차조(2HBOT조)화상압양치료조(NOT조).정상대조조대서8지,기여매조12지대서.제정상대조조외,여각조제작HAPE모형후분별급여HBO간예.사용Western Blot정량검측각조대서폐조직AQP1、AQP5단백수평적표체,동시관찰폐조직병리변화.결과 정상대조조적손상평분위0,HAPE조、1 HBOT조、2HBOT조화NOT조적손상평분분별위(4.46±1.54)、(3.17±1.83)、(1.30 ±0.73)화(3.29±2.27)분；HAPE조교기타각조폐조직손상정도엄중,여정상대조조화2HBOT조비교,차이균유통계학의의(P＜0.01)；2HBOT조여NOT조화1HBOT조비교,폐조직손상정도감경(P＜0.05).정상대조조AQP1화AQP5적회도치위1.00,HAPE조、1HBOT조、2HBOT조화NOT조AQP1적상대회도치분별위(0.58 ±0.20)、(0.76±0.16)、(0.87±0.11)화(0.75±0.27),기AQP5적상대회도치분별위(0.75±0.12)、(0.80±0.14)、(0.96 ±-0.14)화(0.84±0.18)；HAPE조AQP1화AQP5단백수평균교기타각조저,차여정상대조조화2HBOT조간적차이유통계학의의(P＜0.01화P＜0.05)；2HBOT조AQP1화AQP5단백수평여1HBOT조비교,균유제고,차2조간비교,AQP5단백수평차이유통계학의의(P＜0.05).결론 HAPE모형대서폐조직내AQP1、AQP5단백수평표체강저；HBO능구상조AQP1、AQP5적표체,감경폐수종；대우감경폐수종,행2차HBO치료비행1차HBO치료급NOT효과경명현.
Objective To observe the effects of hyperbaric oxygen (HBO) exposure on the expression of aquaporin-1 (AQP1) and aquaporin-5 (AQP5) in rats with simulated high-altitude pulmonary edema (HAPE).Methods Fifty-six rats were randomly divided into five groups:control (normal),HAPE (high altitude pulmonary edema model),1 HBOT (HAPE model and HBO therapy for 1 time),2 HBOT (HAPE model and HBO therapy twice) and NOT (normal pressure oxygen therapy) groups,and was intervened accordingly.Western blotting and real-time PCR techniques were used to analyze the expression of AQP1 and AQP5 in their lungs.The wet-todry (W/D) weight ratio and morphology of the lungs was also examined.Results The protein and gene expression of AQP1 and AQP5 in the HAPE group decreased significantly compared with the control group.There were obvious differences in the protein and mRNA expression of AQP1 and AQP5 between the 2 HBOT group and the HAPE group and between the 2 HBOT group and the 1 HBOT group.Compared with the control group and the 1 HBOT group,marked lung injury could be seen in the HAPE group.Compared with the NOT group and the 1 HBOT group,lung injury in the 2 HBOT group was relieved significantly.Conclusions HAPE in rats is associated with down-regulation of the expression of AQP1 and AQP5 in the lungs.This down-regulation can be attenuated and lung injury can be alleviated by HBOT.Two sessions of HBOT could be more helpful than one for promoting this improvement.