CAJ | 학술논문

目的将新西兰兔制成股四头肌钝挫伤模型,观察按摩损伤侧阳陵泉对受损骨骼肌血管内皮生长因子(VEGF)mRNA表达、微泡超声造影及血液流变的影响.方法采用随机数字表法将42只新西兰大白兔分为正常组(6只)、自然恢复组(18只)及按摩组(18只).选用重物打击法将自然恢复组及按摩组实验兔制成股四头肌急性钝挫伤模型.按摩组实验兔于制模当日开始,在每天下午3:00时给予患侧阳陵泉按摩,每天持续15 min,每天治疗1次.于制模后2h、3d、7d及10d时采用微泡超声影像技术检测各组实验兔局部肌肉病理改变；于制模后3d、7d及10 d时采用逆转录-聚合酶链反应(RT-PCR)检测各组实验兔肌肉组织中VEGF mRNA表达；采用全自动血流变分析仪检测各组实验兔全血黏度及血浆黏度改变.结果制模后3d、7 d及10 d时发现自然恢复组肌肉组织中VEGF mRNA表达较正常组分别增加了35.00％、43.81％、17.94％；按摩组VEGF mRNA表达则较正常组分别增加了94.39％、85.69％、94.20％.微泡超声造影显示按摩组局部损伤修复情况明显优于自然恢复组.血液流变观察结果显示穴位按摩能调节实验兔全身血流状态,加速损伤愈合.结论按摩患侧阳陵泉能改善实验兔局部血供及全身血流状态,加速急性骨骼肌钝挫伤修复.
목적 장신서란토제성고사두기둔좌상모형,관찰안마손상측양릉천대수손골격기혈관내피생장인자(VEGF)mRNA표체、미포초성조영급혈액류변적영향.방법 채용수궤수자표법장42지신서란대백토분위정상조(6지)、자연회복조(18지)급안마조(18지).선용중물타격법장자연회복조급안마조실험토제성고사두기급성둔좌상모형.안마조실험토우제모당일개시,재매천하오3:00시급여환측양릉천안마,매천지속15 min,매천치료1차.우제모후2h、3d、7d급10d시채용미포초성영상기술검측각조실험토국부기육병리개변；우제모후3d、7d급10 d시채용역전록-취합매련반응(RT-PCR)검측각조실험토기육조직중VEGF mRNA표체；채용전자동혈류변분석의검측각조실험토전혈점도급혈장점도개변.결과 제모후3d、7 d급10 d시발현자연회복조기육조직중VEGF mRNA표체교정상조분별증가료35.00％、43.81％、17.94％；안마조VEGF mRNA표체칙교정상조분별증가료94.39％、85.69％、94.20％.미포초성조영현시안마조국부손상수복정황명현우우자연회복조.혈액류변관찰결과현시혈위안마능조절실험토전신혈류상태,가속손상유합.결론 안마환측양릉천능개선실험토국부혈공급전신혈류상태,가속급성골격기둔좌상수복.
Objective To understand the effects of point massage on the expression of vascular endothelial growth factor (VEGF) mRNA expression after skeletal muscle contusion,and also its effects on ultrasonic microbubble imaging and hemorheology.Methods Forty-two New Zealand white rabbits were randomly divided into a normal group (n=6),a self-repairing group (n =18) and a massage group (n =18).Rabbits in the self-repairing and massage groups were hit to generate acute contusion of the quadriceps femoris.Beginning on the same day,the rabbits in the massage group were treated by massage at the Yanglingquan acupoint (GB34) for 15 minutes once daily.Ultrasonic microbubble imaging was used to examine any pathologic changes in the rabbits' muscles at 2 hours and then 3,7 and 10 days after the insult.A reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect VEGF mRNA expression in the rabbits' muscle tissues at days 3,7 and 10.An automatic hemorheology analyzer was employed to detect any changes of in the viscosity of the blood and plasma.Results At days 3,7 and 10,VEGF mRNA expression in the rabbits in the self-repairing group was 35％,44％ and 18％ higher than in the normal group respectively.Average VEGF mRNA expression in the massage group was 94％,86％ and 94％ higher than in the normal group at the same time points.Ultrasonic microbubble imaging suggested that in the massage group local tissue injury repaired much better than in the self-repairing group.The hemorheology observations suggested that point massage could regulate the hemorheology of the rabbits' entire body and hasten the damage recovery process.Conclusion Point massage at the Yanglingquan point (GB34) can hasten the recovery of skeletal muscle contusions by improving local blood supply and whole body hemorheology,at least in rabbits.