CAJ | 학술논문

目的观察早期跑台训练对中重度颅脑外伤大鼠运动功能的影响.方法将32只成年SD大鼠制成中重度脑外伤模型,采用随机数字表法将其分为训练A组、训练B组、训练C组及对照组.训练A组、训练B组及训练C组大鼠分别于术后24 h、3d和7d时进行为期2周的电动跑台训练.于制模后第6、12、18、24及28天时分别采用足误试验(foot-fault)和圆筒试验(cylinder test)评定各组大鼠肢体运动协调能力及前肢运动功能；于制模后第28天时通过焦油紫(CV)染色观察各组大鼠脑体积缺失情况.结果训练A组足误评分在制模后各时间点与对照组间差异均无统计学意义(均P＞0.05)；训练C组足误评分在制模后第12、18、24及28天时分别为(4.70±0.17)分、(4.66 ±0.09)分、(4.81 ±0.14)分和(4.84 ±0.14)分,与对照组间差异均具有统计学意义(均P＜0.05)；训练B组足误评分在制模后第18、24和28天时分别为(4.62±0.17)分、(4.81 ±0.12)分和(4.81±0.09)分,与对照组间差异均具有统计学意义(均P＜0.05).训练C组圆筒试验前肢不对称分值在制模后第12天时开始升高,在制模后第28天时为(0.31±0.04)分,与对照组前肢不对称分值[(0.17±0.04)分0]组间差异具有统计学意义(P＜0.05)；训练A组、训练B组前肢不对称分值在制模后不同时间点与对照组间差异均无统计学意义(均P＞0.05).制模后第28天时发现各跑台训练组大鼠脑体积缺失情况均较对照组有减少趋势,但组间差异均无统计学意义(均P＞0.05).结论于颅脑损伤后3 d或7d时进行为期2周的跑台训练,能改善模型大鼠肢体运动协调能力及前肢运动功能,其治疗过程与脑组织缺失体积无明显相关性.
목적 관찰조기포태훈련대중중도로뇌외상대서운동공능적영향.방법 장32지성년SD대서제성중중도뇌외상모형,채용수궤수자표법장기분위훈련A조、훈련B조、훈련C조급대조조.훈련A조、훈련B조급훈련C조대서분별우술후24 h、3d화7d시진행위기2주적전동포태훈련.우제모후제6、12、18、24급28천시분별채용족오시험(foot-fault)화원통시험(cylinder test)평정각조대서지체운동협조능력급전지운동공능；우제모후제28천시통과초유자(CV)염색관찰각조대서뇌체적결실정황.결과 훈련A조족오평분재제모후각시간점여대조조간차이균무통계학의의(균P＞0.05)；훈련C조족오평분재제모후제12、18、24급28천시분별위(4.70±0.17)분、(4.66 ±0.09)분、(4.81 ±0.14)분화(4.84 ±0.14)분,여대조조간차이균구유통계학의의(균P＜0.05)；훈련B조족오평분재제모후제18、24화28천시분별위(4.62±0.17)분、(4.81 ±0.12)분화(4.81±0.09)분,여대조조간차이균구유통계학의의(균P＜0.05).훈련C조원통시험전지불대칭분치재제모후제12천시개시승고,재제모후제28천시위(0.31±0.04)분,여대조조전지불대칭분치[(0.17±0.04)분0]조간차이구유통계학의의(P＜0.05)；훈련A조、훈련B조전지불대칭분치재제모후불동시간점여대조조간차이균무통계학의의(균P＞0.05).제모후제28천시발현각포태훈련조대서뇌체적결실정황균교대조조유감소추세,단조간차이균무통계학의의(균P＞0.05).결론 우로뇌손상후3 d혹7d시진행위기2주적포태훈련,능개선모형대서지체운동협조능력급전지운동공능,기치료과정여뇌조직결실체적무명현상관성.
Objective To investigate the effect of early rehabilitation on rats' motor performance after moderate or severe experimental traumatic brain injury (TBI).Methods After adult Sprague-Dawley rats had received moderate or severe TBI,they were randomly assigned to either a 24 hour exercise group (group A),a 3-day exercise group (group B),a 7-day exercise group (group C) or a control group.Each group was composed of 8 rats.The exercise group endured forced treadmill training on an electric treadmill for 14 consecutive days initiated 24 hours,3 days or 7 days post TBI.The foot-fault and cylinder tests were performed on the 6th,12th,18th,24th and 28th day post injury.On the 28th day the rats were sacrificed and the brains were removed and frozen.The frozen coronal brain sections were stained with cresyl violet (CV) for quantitative evaluation of cortical lesion volume.Results The rats in groups B and C showed significant decreases in the percentage of left forelimb foot faults compared with the control group at the 18th,24th and28th day after injury.In addition,the rats of group C demonstrated significant decreases in the percentage of left forelimb foot-fault at the 12th day after injury.There was no significant difference between group A and the control group in terms of foot fault scores in any of the subsequent test sessions.In the cylinder test the forelimb use asymmetry scores of neither group A nor group B were significantly different from the control group.Group C showed marked locomotor asymmetry improvement at day 28 post-injury.There was no significantly different loss of hemispheric tissue between those groups.Conclusion After treadmill exercise for 14 consecutive days initiated at 3 or 7 days following moderate or severe TBI,rats' motor function improved.The cortical lesion volume was not associated with recovery of motor function.