CAJ | 학술논문

目的观察针刺对去卵巢骨质疏松症模型大鼠雌二醇、生长素和骨密度的影响.方法选取60只3月龄SD健康雌性大鼠,按随机数字表法分为手术组和假手术组,手术组40只,假手术组20只.采用双侧卵巢切除法复制成质疏松大鼠模型,造模3个月后经双能X线法检测BMD,手术组大鼠腰椎和股骨的骨密度较假手术组明显降低(P＜0.01),表明骨质疏松模型造模成功.造模成功后,从假手术组选取10只大鼠设为对照组,从手术组选取30只大鼠按随机数字表法分为模型组、针刺组和雌激素组,每组10只.其中,针刺组给予电针刺激,雌激素组皮下注射苯甲酸雌二醇,对照组和模型组不作任何处理.连续干预3个月后行骨密度(BMD)检测,然后处死大鼠,摘除眼球取血,检测血清雌二醇、生长素等指标.结果血清生长素:针刺组[(399±163) pg/ml]和雌激素组[(276±92) pg/ml]与模型组[(546 ±98)pg/ml]比较,均有明显降低(P＜0.01);血清雌二醇:针刺组[(128.02±62.28)pg/ml]和雌激素组[(182.89±42.01) pg/ml]与模型组[(72.10±19.83)pg/ml]比较,均有明显提高(P＜0.05);腰椎骨密度:针刺组[(0.212±0.019) g/cm2]和雌激素组[(0.231 ±0.021)g/cm2]与模型组[(0.191±0.012) g/cm2]比较,均有明显提高(P＜0.05),但针刺组对生长素和腰椎骨密度的作用不及雌激素组(P＜0.05).结论针刺疗法能明显提高去卵巢骨质疏松症模型大鼠腰椎骨密度和血清雌二醇的水平,下调血清生长素的水平,但整体作用不及雌激素.
목적 관찰침자대거란소골질소송증모형대서자이순、생장소화골밀도적영향.방법 선취60지3월령SD건강자성대서,안수궤수자표법분위수술조화가수술조,수술조40지,가수술조20지.채용쌍측란소절제법복제성질소송대서모형,조모3개월후경쌍능X선법검측BMD,수술조대서요추화고골적골밀도교가수술조명현강저(P＜0.01),표명골질소송모형조모성공.조모성공후,종가수술조선취10지대서설위대조조,종수술조선취30지대서안수궤수자표법분위모형조、침자조화자격소조,매조10지.기중,침자조급여전침자격,자격소조피하주사분갑산자이순,대조조화모형조불작임하처리.련속간예3개월후행골밀도(BMD)검측,연후처사대서,적제안구취혈,검측혈청자이순、생장소등지표.결과 혈청생장소:침자조[(399±163) pg/ml]화자격소조[(276±92) pg/ml]여모형조[(546 ±98)pg/ml]비교,균유명현강저(P＜0.01);혈청자이순:침자조[(128.02±62.28)pg/ml]화자격소조[(182.89±42.01) pg/ml]여모형조[(72.10±19.83)pg/ml]비교,균유명현제고(P＜0.05);요추골밀도:침자조[(0.212±0.019) g/cm2]화자격소조[(0.231 ±0.021)g/cm2]여모형조[(0.191±0.012) g/cm2]비교,균유명현제고(P＜0.05),단침자조대생장소화요추골밀도적작용불급자격소조(P＜0.05).결론 침자요법능명현제고거란소골질소송증모형대서요추골밀도화혈청자이순적수평,하조혈청생장소적수평,단정체작용불급자격소.
Objective To observe the effect of acupuncture on ghrelin,estradiol (E2) and bone mineral density (BMD) in postmenopausal osteoporosis rats.Methods According to the random number table method,60 female Sprague-Dawley rats aged 3 months,were randomly divided into surgery group (40 rats) and sham group (20 rats).The osteoporosis model was established by bilateral ovariectomy.Three months after the operation,a significant decrease in BMD of lumbar vertebrae and femur of the model rats as measured by dual energy X-ray absorptionmetry against the sham group indicated successful establishment of the osteoporosis model.Then surgery group was randomly divided into a model subgroup,a acupuncture subgroup and an estrogen subgroup,with 10 rats in each subgroup.The acupuncture subgroup was treated with electroacupuncture,estrogen subgroup with subcutaneous injection of estradiol benzoate,sham group and model subgroup without any treatment.After 3 months of intervention all the rats were sacrificed,BMD of the lumbar vertebrae and femur was measured,ghrelin and E2 were determined.Results Serum ghrelin in acupuncture subgroup (399 pg/ml) and estrogen subgroup (276 pg/ml) were significantly lower (P ＜ 0.01) than that in model subgroup (546 pg/ml); serum E2 in acupuncture subgroup (128.02 pg/ml) and estrogen subgroup (182.89 pg/ml) increased significantly (P ＜ 0.05) than that in model subgroup (72.10 pg/ml) ;lumbar vertebrae BMD in acupuncture subgroup (0.212 g/cm2) and estrogen subgroup (0.231 g/cm2) increased significantly (P ＜ 0.05) than that in model subgroup (0.191 g/cm2),but in acupuncture subgroup the effect on improving the level of E2,ghrelin and lumbar vertebrae BMD was less than that in estrogen subgroup (P ＜ 0.05).Conclusions Acupuncture intervention can improve lumbar vertebrae BMD and serum E2 level of postmenopausal osteoporosis rat model,down regulate serum ghrelin level,but the overall effect of acupuncture is less than estrogen.