CAJ | 학술논문

目的探讨磁场对慢性不可预知的温和应激(CUMS)模型大鼠行为学的影响及其作用机制.方法将36只Wistar大鼠按随机数字表法分为模型组、磁场组和对照组,每组大鼠12只.对照组不予任何刺激,常规饲养9周;模型组和磁场组大鼠按照CUMS的方法每日随机给予一种刺激,连续刺激5周.入组5周后,将磁场组鼠置于旋磁机中心,模型组则常规饲养4周.3组大鼠均于入组当天、入组5周后、入组9周后,进行常规、行为学和血尿指标检测.结果入组5周后,对照组的糖水消耗量为(32.4±16.7)ml/24 h,与模型组的(25.8 ±19.7)ml/24 h和磁场组的(26.2±18.4) ml/24 h比较,差异均有统计学意义(P＜0.05);入组5周后和入组9周后,模型组和磁场组大鼠的体重均显著低于对照组(P＜0.05),入组9周后,磁场组大鼠体重显著高于模型组(P＜0.05);3组大鼠存活数量比较采用Fisher确切概率法,3组比较,差异均无统计学意义(P＞0.05).入组9周后,模型组和磁场组旷场、悬尾实验各项指标与对照组比较,差异均有统计学意义(P＜0.05),磁场组的穿格次数、理毛时间、悬尾不动时间与模型组比较,差异亦有统计学意义(P＜0.05).入组5周后,磁场组和模型组尿皮质醇水平均高于对照组(P＜0.05);入组9周后,磁场组血皮质醇含量与模型组比较,差异有统计学意义(P＜0.05).结论 400 mT的磁场可减轻慢性应激所致大鼠抑郁状态时行为变化的程度,其机制可能与影响血、尿皮质醇水平有关.
목적 탐토자장대만성불가예지적온화응격(CUMS)모형대서행위학적영향급기작용궤제.방법 장36지Wistar대서안수궤수자표법분위모형조、자장조화대조조,매조대서12지.대조조불여임하자격,상규사양9주;모형조화자장조대서안조CUMS적방법매일수궤급여일충자격,련속자격5주.입조5주후,장자장조서치우선자궤중심,모형조칙상규사양4주.3조대서균우입조당천、입조5주후、입조9주후,진행상규、행위학화혈뇨지표검측.결과 입조5주후,대조조적당수소모량위(32.4±16.7)ml/24 h,여모형조적(25.8 ±19.7)ml/24 h화자장조적(26.2±18.4) ml/24 h비교,차이균유통계학의의(P＜0.05);입조5주후화입조9주후,모형조화자장조대서적체중균현저저우대조조(P＜0.05),입조9주후,자장조대서체중현저고우모형조(P＜0.05);3조대서존활수량비교채용Fisher학절개솔법,3조비교,차이균무통계학의의(P＞0.05).입조9주후,모형조화자장조광장、현미실험각항지표여대조조비교,차이균유통계학의의(P＜0.05),자장조적천격차수、리모시간、현미불동시간여모형조비교,차이역유통계학의의(P＜0.05).입조5주후,자장조화모형조뇨피질순수평균고우대조조(P＜0.05);입조9주후,자장조혈피질순함량여모형조비교,차이유통계학의의(P＜0.05).결론 400 mT적자장가감경만성응격소치대서억욱상태시행위변화적정도,기궤제가능여영향혈、뇨피질순수평유관.
Objective To study influences of magnetic field on behavior of depressive rats under chronic unpredictable mild stress,and possible mechanisms under such phenomena.Methods Thirty-six Wistar rats were divided into model group,magnetic field group and control group (n =12 in each group) according to random number table.The model group and the magnetic field group received CUMS stimulation daily for 5 weeks,while the control group received no any treatment.At the end of the fifth week,rats in the magnetic field group were placed in the center of a magnetic machine (400 mT),and the model group was raised freely for4 weeks.The severity of depressive behaviors,cortisol levels and contents of C-reactive protein in blood and urine sample were tested pre-treatment,at the end of 5 weeks and 9 weeks.Results After 5 weeks,consumption of glucose solution of the control group was higher than the other two groups (P ＜ 0.05).After 5 and 9 weeks,body weights of the magnetic group and the model group were lower than the control group (P ＜ 0.05).At the end of 9 weeks,performance of the magnetic group as indicated by frequency of grid crossing,grooming time and suspending tail test were improved to a significantly greater extent than the other two groups (P ＜ 0.05).In addition,the urine content of cortisol in 24 hours in magnetic field and model groups was higher than the control group at the end of 5 weeks,and the serum level of cortisol in the magnetic field group were significantly higher than the model group at the end of 9 weeks (P ＜ 0.05).Conclusion The 400mT magnetic field can reduce the severity of behavior changes of rats with CUMS-induced depression,which might be attributable to its influence on the cortisol levels in blood and urine.