CAJ | 학술논문

目的探讨高压氧(HBO)治疗对β-淀粉样蛋白(Aβ) 25-35所致拟阿尔茨海默病(AD)模型大鼠认知和记忆功能的改变及其海马神经元凋亡情况的影响.方法选取健康成年雄性Sprague Dawley(SD)大鼠48只,按随机数字表法分为正常对照组、假手术组、模型组和HBO治疗组,每组12只.正常对照组不做任何处理.其余各组大鼠给予10％水合氯醛(4 ml)腹腔注射麻醉,假手术组大鼠每侧海马注射5μl生理盐水;造模大鼠(模型组和HBO治疗组)每侧海马注射5μl的Aβ25-35制备拟AD大鼠痴呆模型.造模成功后,模型组大鼠不做任何治疗处理;HBO治疗组大鼠造模2周后,常规HBO治疗,每日1次,10d为1个疗程,中间休息3d,共2个疗程.采用Morris水迷宫法观察各组大鼠空间记忆能力的改变,TUNEL染色观察大鼠海马神经元凋亡情况的改变,同时检测海马组织凋亡相关基因Bcl-2和Bax的mRNA、蛋白表达的改变.结果水迷宫实验中,第5天和第6天HBO治疗组大鼠的逃避潜伏期与模型组比较显著缩短[(33.4±4.5)s比(48.1±2.7)s,(20.8±1.7)s比(40.5±1.9)s,P＜O.05],空间探索实验中HBO治疗组大鼠在原平台所在象限的时间及穿过原平台的次数较模型组显著增加[(35.8±5.6)％比(21.1±3.8)％,(4.8±1.1)次比(3.1±1.2)次,P＜0.05].TUNEL染色中,模型组海马神经元中胞核呈现棕色凋亡形态的较多,而HBO治疗组则见少数凋亡的海马神经元.海马组织凋亡基因检测中,HBO治疗组Bcl-2 mRNA和蛋白的表达显著高于模型组(P＜0.05),其Bax mRNA和蛋白的表达则相应地降低.结论 HBO可能通过抑制Aβ25-35诱导的海马神经元凋亡而改善AD大鼠模型的认知和记忆能力.
목적 탐토고압양(HBO)치료대β-정분양단백(Aβ) 25-35소치의아이자해묵병(AD)모형대서인지화기억공능적개변급기해마신경원조망정황적영향.방법 선취건강성년웅성Sprague Dawley(SD)대서48지,안수궤수자표법분위정상대조조、가수술조、모형조화HBO치료조,매조12지.정상대조조불주임하처리.기여각조대서급여10％수합록철(4 ml)복강주사마취,가수술조대서매측해마주사5μl생리염수;조모대서(모형조화HBO치료조)매측해마주사5μl적Aβ25-35제비의AD대서치태모형.조모성공후,모형조대서불주임하치료처리;HBO치료조대서조모2주후,상규HBO치료,매일1차,10d위1개료정,중간휴식3d,공2개료정.채용Morris수미궁법관찰각조대서공간기억능력적개변,TUNEL염색관찰대서해마신경원조망정황적개변,동시검측해마조직조망상관기인Bcl-2화Bax적mRNA、단백표체적개변.결과 수미궁실험중,제5천화제6천HBO치료조대서적도피잠복기여모형조비교현저축단[(33.4±4.5)s비(48.1±2.7)s,(20.8±1.7)s비(40.5±1.9)s,P＜O.05],공간탐색실험중HBO치료조대서재원평태소재상한적시간급천과원평태적차수교모형조현저증가[(35.8±5.6)％비(21.1±3.8)％,(4.8±1.1)차비(3.1±1.2)차,P＜0.05].TUNEL염색중,모형조해마신경원중포핵정현종색조망형태적교다,이HBO치료조칙견소수조망적해마신경원.해마조직조망기인검측중,HBO치료조Bcl-2 mRNA화단백적표체현저고우모형조(P＜0.05),기Bax mRNA화단백적표체칙상응지강저.결론 HBO가능통과억제Aβ25-35유도적해마신경원조망이개선AD대서모형적인지화기억능력.
Objective To explore the possible protective effect of hyperbaric oxygen (HBO) on cognitive deficits induced by amyloid β25-35 (Aβ25-35) and neuronal apoptosis in the hippocampi of rats with Alzheimer's disease (AD).Methods The animal AD model was established in 24 Sprague-Dawley rats by bilateral hippocampal injection of Aβ25-35.Twelve rats were injected with normal saline as controls,and another 12 served as normal controls.After the injection,the model rats were further divided into a model group and a treatment group.All the rats were housed with normal feeding for 2 weeks and then those in the treatment groups received a total of 2 courses of HBO treatment (10 days each with an interval of 3 days in between).The other groups were left with no treatment.After the treatment,the rats' learning and memory ability were tested using Morris' water maze test,and any neuronal changes were observed using TUNEL staining.The expression of mRNA and Bcl-2 and Bax proteins in the hippocampus were detected using a RT-PCR and Western blotting.Results HBO significantly improved the learning and memory impairment and alleviated neuronal apoptosis in the hippocampus compared against the control group.In addition,HBO treatment significantly increased the mRNA and protein expression of Bcl-2 and down-regulated the expression of Bax.Conclusion HBO treatment can prevent learning and memory impairment induced by Aβ25-35 peptides,which might be mediated by inhibiting neuronal apoptosis in the hippocampus.