中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
1期
12-15
,共4页
李砚屏%刘青乐%郑成刚%衣洪杰%赵黎明%杭晓华
李硯屏%劉青樂%鄭成剛%衣洪傑%趙黎明%杭曉華
리연병%류청악%정성강%의홍걸%조려명%항효화
高压氧%急性一氧化碳中毒%迟发性脑病%髓鞘碱性蛋白
高壓氧%急性一氧化碳中毒%遲髮性腦病%髓鞘堿性蛋白
고압양%급성일양화탄중독%지발성뇌병%수초감성단백
Hyperbaric oxygen%Carbon monoxide poisoning%Myelin basic protein%Memory%Learning
目的 观察高压氧(HBO)对急性一氧化碳中毒(COP)迟发性脑病大鼠学习记忆及脑组织髓鞘碱性蛋白(MBP)表达的影响.方法 选取雄性SD大鼠45只,经Morris水迷宫筛选后,分为正常对照组(NC组)11只、急性一氧化碳中毒组(COP组)17只和急性一氧化碳中毒高压氧治疗组(HB0组)17只.COP组和HBO组采用分次腹腔注射CO气体法建立大鼠急性COP迟发性脑病模型,NC组采用相同方法腹腔注射等量空气.造模成功后,HBO组大鼠进行HBO治疗,NC组及COP组均给予常压饲养.与造模成功后第1天至造模成功后第21天对3组大鼠进行水迷宫测试,观察其学习记忆能力的变化情况,并于造模成功后第21天检测3组大鼠脑组织中MBP的表达情况.结果 造模后21 d内,COP组迟发性脑病发病9只(64.3％),HB0组发病4只(26.7％),2组间差异有统计学意义(P＜0.05).HBO组大鼠平均逃避潜伏期为(6.20±1.98)s,与COP组的(10.61±4.82)s比较,差异有统计学意义(P＜0.05).造模成功后第21天,COP组和HBO组大鼠脑组织MBP的表达均低于NC组(P＜0.05);COP组大鼠脑组织MBP的表达低于HBO组(P＜0.05).3组大鼠脑组织灰度值比较,NC组显著优于COP组和HBO组(P＜0.05),而HBO组亦显著优于COP组(P＜0.05).结论 HBO治疗能改善急性COP迟发性脑病大鼠学习记忆能力,减轻脑组织脱髓鞘损伤.
目的 觀察高壓氧(HBO)對急性一氧化碳中毒(COP)遲髮性腦病大鼠學習記憶及腦組織髓鞘堿性蛋白(MBP)錶達的影響.方法 選取雄性SD大鼠45隻,經Morris水迷宮篩選後,分為正常對照組(NC組)11隻、急性一氧化碳中毒組(COP組)17隻和急性一氧化碳中毒高壓氧治療組(HB0組)17隻.COP組和HBO組採用分次腹腔註射CO氣體法建立大鼠急性COP遲髮性腦病模型,NC組採用相同方法腹腔註射等量空氣.造模成功後,HBO組大鼠進行HBO治療,NC組及COP組均給予常壓飼養.與造模成功後第1天至造模成功後第21天對3組大鼠進行水迷宮測試,觀察其學習記憶能力的變化情況,併于造模成功後第21天檢測3組大鼠腦組織中MBP的錶達情況.結果 造模後21 d內,COP組遲髮性腦病髮病9隻(64.3％),HB0組髮病4隻(26.7％),2組間差異有統計學意義(P＜0.05).HBO組大鼠平均逃避潛伏期為(6.20±1.98)s,與COP組的(10.61±4.82)s比較,差異有統計學意義(P＜0.05).造模成功後第21天,COP組和HBO組大鼠腦組織MBP的錶達均低于NC組(P＜0.05);COP組大鼠腦組織MBP的錶達低于HBO組(P＜0.05).3組大鼠腦組織灰度值比較,NC組顯著優于COP組和HBO組(P＜0.05),而HBO組亦顯著優于COP組(P＜0.05).結論 HBO治療能改善急性COP遲髮性腦病大鼠學習記憶能力,減輕腦組織脫髓鞘損傷.
목적 관찰고압양(HBO)대급성일양화탄중독(COP)지발성뇌병대서학습기억급뇌조직수초감성단백(MBP)표체적영향.방법 선취웅성SD대서45지,경Morris수미궁사선후,분위정상대조조(NC조)11지、급성일양화탄중독조(COP조)17지화급성일양화탄중독고압양치료조(HB0조)17지.COP조화HBO조채용분차복강주사CO기체법건립대서급성COP지발성뇌병모형,NC조채용상동방법복강주사등량공기.조모성공후,HBO조대서진행HBO치료,NC조급COP조균급여상압사양.여조모성공후제1천지조모성공후제21천대3조대서진행수미궁측시,관찰기학습기억능력적변화정황,병우조모성공후제21천검측3조대서뇌조직중MBP적표체정황.결과 조모후21 d내,COP조지발성뇌병발병9지(64.3％),HB0조발병4지(26.7％),2조간차이유통계학의의(P＜0.05).HBO조대서평균도피잠복기위(6.20±1.98)s,여COP조적(10.61±4.82)s비교,차이유통계학의의(P＜0.05).조모성공후제21천,COP조화HBO조대서뇌조직MBP적표체균저우NC조(P＜0.05);COP조대서뇌조직MBP적표체저우HBO조(P＜0.05).3조대서뇌조직회도치비교,NC조현저우우COP조화HBO조(P＜0.05),이HBO조역현저우우COP조(P＜0.05).결론 HBO치료능개선급성COP지발성뇌병대서학습기억능력,감경뇌조직탈수초손상.
Objective To observe the effects of hyperbaric oxygen (HBO) on learning and memory ability and on the level of myelin basic protein (MBP) in the brain tissue of rats with delayed neurological sequelae (DNS) after acute carbon monoxide poisoning (COP).Methods Forty-eight male SD rats had their cognitive performance assessed with the Morris water maze.After basic training with the Morris water maze and screening,the rats were randomly divided intoanormalcontrol (NC) group (n=11),a COP group (n=17) and an HBO group (n=17).Pure CO gas was injected intraperitoneally to establish acute COP in the latter two groups.The NC group received a similar injection of air.The HBO group was given HBO therapy.The rats in each group were tested for changes in their learning and memory abilities using the Morris water maze.On the 21 st day after the treatment,paraffin tissue sections of the rats' brains were subjected to immunohistochemical (IHC) examination and Western blotting (WB) was used to detect any expression of MBP in the brain tissue.Results After 21 days,morbidity among the COP group was 64.3％,while it was 26.7％ in the HBO group,a significant difference.The average maze escape latency in the COP group was significantly longer than in the HBO group.IHC staining and Western blotting showed that MBP in the hippocampal tissue of the COP group was significantly lower than that in the HBO group.In gray scale comparisons of the rats' brain tissue,that from the NC group was significantly better than that from the COP and HBO groups,but that from the HBO group was significantly better than that from the COP group.Conclusion HBO can effectively reduce DNS after acute COP,mitigate the severity of DNS,reduce demyelination of brain tissue and thus play an important role in protecting brain cells.
