经肋间动脉移植骨髓基质细胞对家兔脊髓损伤后神经轴突及胶质瘢痕的影响
경륵간동맥이식골수기질세포대가토척수손상후신경축돌급효질반흔적영향
Effects of transplanting bone marrow stromal cells on axonal and glial scarring after spinal cord injury
  • 万方数据
    中华物理医学与康复杂志 중화물리의학여강복잡지
    CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
    2014年 1期 25-30 ,共6页

    王晶%周晓忠%李超%孙鸿涛%陈为坚%李贵涛

    왕정%주효충%리초%손홍도%진위견%리귀도

    脊髓损伤%肋间动脉%骨髓基质细胞%神经微丝%胶质纤维酸性蛋白%家兔 脊髓損傷%肋間動脈%骨髓基質細胞%神經微絲%膠質纖維痠性蛋白%傢兔
    척수손상%륵간동맥%골수기질세포%신경미사%효질섬유산성단백%가토
    Spinal cord injury%Intercostal arteries%Bone marrow stromal cells%Neurofilaments%Glial fibrillary acidic protein%Rabbits
    目的 探讨经肋间动脉移植骨髓基质细胞(BMSC)对家兔脊髓损伤(SCI)后神经轴突及胶质瘢痕的影响.方法 采用随机数字表法将30只家兔分为假手术组、损伤对照组和BMSC治疗组.采用钳夹法将损伤对照组及BMSC治疗组制成T9 SCI动物模型,假手术组仅打开椎板,不损伤脊髓.BMSC治疗组和损伤对照组于制模后1周时分别经肋间动脉注射0.5 ml BMSC细胞悬液和等量生理盐水.于制模后第1天、第1周、第2周和第4周时分别采用BBB评分评定各组家兔后肢运动功能,于制模后4周时提取各组家兔受损脊髓行HE和Nissl染色,观察脊髓病理形态改变;并采用免疫组化法观察各组家兔受损脊髓神经丝蛋白(N F200)和胶原纤维酸性蛋白(GFAP)变化.结果 制模后不同时间点发现假手术组BBB评分均明显高于损伤对照组及BMSC治疗组(P ＜0.05);BMSC治疗组制模后第2周、第4周时BBB评分[分别是(8.38±0.97)分和(14.63 ±1.77)分]均明显高于损伤对照组[分别是(6.38 ±1.07)分和(8.50±0.93)分](P＜0.05).HE染色显示,制模后第4周时假手术组脊髓内未发现胶质瘢痕及空洞形成;损伤对照组和BMSC治疗组SCI区域均可见胶质细胞增生、胶质瘢痕及空洞形成,并且BMSC治疗组病理改变较损伤对照组减轻.Nissl染色显示假手术组典型神经元数量较多,损伤对照组及BMSC治疗组均有大量神经元碎裂、降解,其中BMSC治疗组的残存神经元数量明显多于损伤对照组;免疫组化检查提示损伤对照组、BMSC治疗组NF200阳性细胞数及GFAP阳性染色面积均较假手术组明显增加(P＜0.05),其中BMSC治疗组受损脊髓内NF200阳性细胞数[(57.88±9.76)％]明显高于损伤对照组[(21.25±4.50)％](P＜0.05);同时BMSC治疗组GFAP阳性染色面积[(3154.01 ±334.47) μm2]明显小于损伤对照组[(4536.79±686.83)μm2](P＜0.05).结论 经肋间动脉移植BMSC能促进SCI家兔受损脊髓神经轴突生长,抑制胶质细胞增生及胶质瘢痕形成,有助于脊髓神经功能恢复.
    목적 탐토경륵간동맥이식골수기질세포(BMSC)대가토척수손상(SCI)후신경축돌급효질반흔적영향.방법 채용수궤수자표법장30지가토분위가수술조、손상대조조화BMSC치료조.채용겸협법장손상대조조급BMSC치료조제성T9 SCI동물모형,가수술조부타개추판,불손상척수.BMSC치료조화손상대조조우제모후1주시분별경륵간동맥주사0.5 ml BMSC세포현액화등량생리염수.우제모후제1천、제1주、제2주화제4주시분별채용BBB평분평정각조가토후지운동공능,우제모후4주시제취각조가토수손척수행HE화Nissl염색,관찰척수병리형태개변;병채용면역조화법관찰각조가토수손척수신경사단백(N F200)화효원섬유산성단백(GFAP)변화.결과 제모후불동시간점발현가수술조BBB평분균명현고우손상대조조급BMSC치료조(P ＜0.05);BMSC치료조제모후제2주、제4주시BBB평분[분별시(8.38±0.97)분화(14.63 ±1.77)분]균명현고우손상대조조[분별시(6.38 ±1.07)분화(8.50±0.93)분](P＜0.05).HE염색현시,제모후제4주시가수술조척수내미발현효질반흔급공동형성;손상대조조화BMSC치료조SCI구역균가견효질세포증생、효질반흔급공동형성,병차BMSC치료조병리개변교손상대조조감경.Nissl염색현시가수술조전형신경원수량교다,손상대조조급BMSC치료조균유대량신경원쇄렬、강해,기중BMSC치료조적잔존신경원수량명현다우손상대조조;면역조화검사제시손상대조조、BMSC치료조NF200양성세포수급GFAP양성염색면적균교가수술조명현증가(P＜0.05),기중BMSC치료조수손척수내NF200양성세포수[(57.88±9.76)％]명현고우손상대조조[(21.25±4.50)％](P＜0.05);동시BMSC치료조GFAP양성염색면적[(3154.01 ±334.47) μm2]명현소우손상대조조[(4536.79±686.83)μm2](P＜0.05).결론 경륵간동맥이식BMSC능촉진SCI가토수손척수신경축돌생장,억제효질세포증생급효질반흔형성,유조우척수신경공능회복.
    Objective To investigate the effects of bone marrow stromal cell (BMSC) transplantation on axonal and glial scarring after spinal cord injury (SCI).Methods Thirty New Zealand white rabbits were randomly assigned to a sham operation group (group A),a saline treatment group (group B) or a BMSC treatment group (group C).Group A served as controls,in which the canal was opened without damage to the spinal cord.In groups C and B SCI models were established with aneurysm clips and the rabbits of groups C and B were then given injections of BMSCs and saline solution respectively via the intra-intercostal artery at 1 week post injury.At 1 day,1 week,2 weeks and 4 weeks post injury,Basso Beattie-Bresnahan (BBB) scores were assessed to evaluate the recovery of locomotor function in the hind limbs.Spinal cord samples were harvested for HE and Nissl staining,and immunohistochemistry and image analysis were used to detect any changes in neurofilament (NF200) and glial fibrillary acidic protein (GFAP) in the injured spinal cords.Results The average BBB scores of group A were significantly higher those that of groups B and C at each time point,and those of group C were significantly better than those of group B at the 2nd and 4th week post injury.At the 4th week post injury,HE staining showed there was no glial scarring or cavities in group A,but that there was glial cellular proliferation,glial scarring and cavity formation at the injury site in groups B and C.In group C all were obviously less than in group B.Nissl staining indicated there were more typical neurons in group A,while there were a larger number of ruptured neurons,more degradation,and irregular remaining neurons in groups B and C.These abnormalities were again significantly more prevalent in group C.Immunohistochemical examination showed significant increases in NF200 positive neurons and GFAP in groups B and C compared with group A.The number of NF200 positive neurons was significantly higher in group C than in group B,but the GFAP positive area was significantly smaller in group C than in group B.Conclusion BMSC transplantation via the intercostal arteries can effectively improve axonal regeneration,attenuate glial cellular proliferation and reduce glial scar formation,promoting functional recovery after SCI,at least in rabbits.
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