中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
7期
595-599
,共5页
李世军%余春%王定明%唐青%陶晓燕%李浩%庄妍%周敬祝%王月%田克诚%唐光鹏
李世軍%餘春%王定明%唐青%陶曉燕%李浩%莊妍%週敬祝%王月%田剋誠%唐光鵬
리세군%여춘%왕정명%당청%도효연%리호%장연%주경축%왕월%전극성%당광붕
狂犬病病毒%糖蛋白基因%疫苗毒株%差异
狂犬病病毒%糖蛋白基因%疫苗毒株%差異
광견병병독%당단백기인%역묘독주%차이
Rabies virus%Glycoprotein gene%Vaccine strain%Differences
目的 分析贵州省狂犬病病毒流行毒株与疫苗毒株的糖蛋白基因(G基因)差异,为狂犬病疫苗研制及有效防控措施的制定提供科学依据.方法 以RT-PCR扩增贵州省近几年人和犬狂犬病病毒阳性脑组织标本G基因序列,对扩增产物进行序列测定后采用生物信息学软件对序列与疫苗株G基因序列进行比较分析.结果 贵州省狂犬病病毒阳性标本经RT-PCR扩增、测序与拼接后得到8株狂犬病病毒G基因全长序列,与我国常用狂犬病疫苗毒株在核苷酸和推导的氨基酸水平上的同源性分别为82.0％ ～94.1％和87.6％ ～ 97.5％,与人用疫苗株中的CTN株G基因核苷酸和氨基酸序列同源性最高(分别为87.0％ ～ 94.1％和93.7.％～97.5％),而在兽用疫苗株中与Flury株的核苷酸和氨基酸同源性最高(分别为83.9％ ～ 84.6％和91.1％ ～ 93.0％).在所测定序列中以来自贵州省2005年的GZ09与人用疫苗毒株CTN和动物用疫苗株Flury株G同源性最高,而以2010年的GZ30与人用疫苗毒株CTN和动物用疫苗株Flury株G基因同源性最低.系统进化树分析显示,本次所测定的序列和9株疫苗株与狂犬病病毒基因1～7型中的基因1型代表毒株聚类在同一分支,与疫苗毒株中的CTN亲缘进化关系最近,而与其他疫苗株相对较远,所测定序列中以2005年的GZ09与CTN疫苗株同源性最近,而其他序列与CTN毒株的亲缘进化关系相对较远.结论 本研究从狂犬病病毒G基因水平证明贵州省狂犬病病毒流行毒株与常用的疫苗株同属狂犬病病毒基因1型,与人用疫苗株中的CTN毒株及兽用疫苗株中的Flury株差异最小,且随着时间的推移贵州省狂犬病病毒流行毒株与疫苗毒株G基因的差异越来越大,该研究结果将为狂犬病疫苗研制及有效防控措施的制定提供科学依据.
目的 分析貴州省狂犬病病毒流行毒株與疫苗毒株的糖蛋白基因(G基因)差異,為狂犬病疫苗研製及有效防控措施的製定提供科學依據.方法 以RT-PCR擴增貴州省近幾年人和犬狂犬病病毒暘性腦組織標本G基因序列,對擴增產物進行序列測定後採用生物信息學軟件對序列與疫苗株G基因序列進行比較分析.結果 貴州省狂犬病病毒暘性標本經RT-PCR擴增、測序與拼接後得到8株狂犬病病毒G基因全長序列,與我國常用狂犬病疫苗毒株在覈苷痠和推導的氨基痠水平上的同源性分彆為82.0％ ～94.1％和87.6％ ～ 97.5％,與人用疫苗株中的CTN株G基因覈苷痠和氨基痠序列同源性最高(分彆為87.0％ ～ 94.1％和93.7.％～97.5％),而在獸用疫苗株中與Flury株的覈苷痠和氨基痠同源性最高(分彆為83.9％ ～ 84.6％和91.1％ ～ 93.0％).在所測定序列中以來自貴州省2005年的GZ09與人用疫苗毒株CTN和動物用疫苗株Flury株G同源性最高,而以2010年的GZ30與人用疫苗毒株CTN和動物用疫苗株Flury株G基因同源性最低.繫統進化樹分析顯示,本次所測定的序列和9株疫苗株與狂犬病病毒基因1～7型中的基因1型代錶毒株聚類在同一分支,與疫苗毒株中的CTN親緣進化關繫最近,而與其他疫苗株相對較遠,所測定序列中以2005年的GZ09與CTN疫苗株同源性最近,而其他序列與CTN毒株的親緣進化關繫相對較遠.結論 本研究從狂犬病病毒G基因水平證明貴州省狂犬病病毒流行毒株與常用的疫苗株同屬狂犬病病毒基因1型,與人用疫苗株中的CTN毒株及獸用疫苗株中的Flury株差異最小,且隨著時間的推移貴州省狂犬病病毒流行毒株與疫苗毒株G基因的差異越來越大,該研究結果將為狂犬病疫苗研製及有效防控措施的製定提供科學依據.
목적 분석귀주성광견병병독류행독주여역묘독주적당단백기인(G기인)차이,위광견병역묘연제급유효방공조시적제정제공과학의거.방법 이RT-PCR확증귀주성근궤년인화견광견병병독양성뇌조직표본G기인서렬,대확증산물진행서렬측정후채용생물신식학연건대서렬여역묘주G기인서렬진행비교분석.결과 귀주성광견병병독양성표본경RT-PCR확증、측서여병접후득도8주광견병병독G기인전장서렬,여아국상용광견병역묘독주재핵감산화추도적안기산수평상적동원성분별위82.0％ ～94.1％화87.6％ ～ 97.5％,여인용역묘주중적CTN주G기인핵감산화안기산서렬동원성최고(분별위87.0％ ～ 94.1％화93.7.％～97.5％),이재수용역묘주중여Flury주적핵감산화안기산동원성최고(분별위83.9％ ～ 84.6％화91.1％ ～ 93.0％).재소측정서렬중이래자귀주성2005년적GZ09여인용역묘독주CTN화동물용역묘주Flury주G동원성최고,이이2010년적GZ30여인용역묘독주CTN화동물용역묘주Flury주G기인동원성최저.계통진화수분석현시,본차소측정적서렬화9주역묘주여광견병병독기인1～7형중적기인1형대표독주취류재동일분지,여역묘독주중적CTN친연진화관계최근,이여기타역묘주상대교원,소측정서렬중이2005년적GZ09여CTN역묘주동원성최근,이기타서렬여CTN독주적친연진화관계상대교원.결론 본연구종광견병병독G기인수평증명귀주성광견병병독류행독주여상용적역묘주동속광견병병독기인1형,여인용역묘주중적CTN독주급수용역묘주중적Flury주차이최소,차수착시간적추이귀주성광견병병독류행독주여역묘독주G기인적차이월래월대,해연구결과장위광견병역묘연제급유효방공조시적제정제공과학의거.
Objective To study the differences of glycoprotein gene (G gene) between rabies virus epidemic strains of Guizhou province in recent years and vaccine strains,and to provide scientific basis for the development of rabies vaccine and establishment of effective control and prevention measures.Methods RT-PCR assay was used to amplify the G gene of rabies positive brain tissues samples of human and dog derived from Guizhou province in recent years.The amplification products were sequenced and comparatively analyzed with that of vaccine strains by using bioinformatics software.Results Eight full-length G gene sequences were obtained by RT-PCR amplification,sequencing and splicing.The homogeny of G gene between 8 epidemic strains of Guizhou province and 9 vaccine strains were 82.0％-94.1％ and 87.6％-97.5％ on nucleotide and deduced amino acid level,respectively,and the highest homogeny were found with the human vaccine strain CTN (87.0％-94.1％ for nucleotide and 93.7.％-97.5％ for amino acid) among the 6 human rabies vaccine strains,while highest homogeny were found with strain Flury (83.9％-84.6％ for nucleotide and 91.1％-93.0％ for amino acid) among the three animal vaccine strains.Besides,among the 8 epidemic strains from Guizhou province,strain GZ09 collected in the year of 2005 was of the highest homogeny with human rabies vaccine strain CTN and animal rabies vaccine strain Flury,while strain GZ30 collected in the year of 2010 was of lowest homogeny with human rabies vaccine strain CTN and animal rabies vaccine strain Flury.Moreover,phylogenetic analysis based on the G gene indicated that the relationship of 8 epidemic strains derived from Guizhou,the 9 vaccine strains and genotype 1 Lyssavirus were clustered to a same branch.Vaccine strain CTN among the 9 vaccine strains was closest to the 8 epidemic strains,and the other 8 vaccine strains were relatively more distant from the epidemic strains of Guizhou province.In addition,phylogenetic analysis indicated that among the 8 epidemic strains from Guizhou province,strain GZ09 collectcd in the year of 2005 was of closest evolutionary relationship to CTN,while the other 7 epidemic strains were relatively more distant from CTN.Conclusion This study confirmed on the G gene level that rabies virus strains circulated in Guizhou province in recent years and the vaccine strains used in China belonged to rabies virus genotype 1,and the virus strains circulated in Guizhou province in recent years is of smallest difference with the human vaccine strain CTN and animal vaccine strain Flury.Besides,as time goes on,the difference between the epidemic strain and the vaccine strains becomes more and more obvious.The results of this study will provide scientific basis for the development of rabies vaccine and establishment of effective control and prevention measures.
