CAJ | 학술논문

目的了解贵阳地区GⅡ.4型诺如病毒变异株的组成及其点突变.方法监测2010年6-11月于贵阳地区哨点医院就诊的急性胃肠炎病例,收集粪便标本,采用荧光定量逆转录-聚合酶链反应(real-time RT-PCR)初步鉴定,随机选取部分GⅡ型诺如病毒阳性标本,采用一步法RT-PCR对其VP1基因区克隆及测序,基因序列与国内外流行的GⅡ.4型诺如病毒进行系统进化及氨基酸位点的突变分析.结果检测标本426份,有267份(62.68％)GⅡ型诺如病毒阳性,测序获得了9份GⅡ.4型诺如病毒VP1基因组序列,贵州省2010年流行的GⅡ.4型诺如病毒包括2个变异株(GⅡ.42008a和GⅡ.4 2008b新型变异株),亚型组闻的VP1基因的同源性为95.90％～ 96.72％,亚型组内同源性为99.45％～ 100％,有2个氨基酸位点易发生突变.结论贵阳地区2010年夏秋季急性胃肠炎以诺如病毒GⅡ型为主,并且变异株具有多样性.
목적 료해귀양지구GⅡ.4형낙여병독변이주적조성급기점돌변.방법 감측2010년6-11월우귀양지구초점의원취진적급성위장염병례,수집분편표본,채용형광정량역전록-취합매련반응(real-time RT-PCR)초보감정,수궤선취부분GⅡ형낙여병독양성표본,채용일보법RT-PCR대기VP1기인구극륭급측서,기인서렬여국내외류행적GⅡ.4형낙여병독진행계통진화급안기산위점적돌변분석.결과 검측표본426빈,유267빈(62.68％)GⅡ형낙여병독양성,측서획득료9빈GⅡ.4형낙여병독VP1기인조서렬,귀주성2010년류행적GⅡ.4형낙여병독포괄2개변이주(GⅡ.42008a화GⅡ.4 2008b신형변이주),아형조문적VP1기인적동원성위95.90％～ 96.72％,아형조내동원성위99.45％～ 100％,유2개안기산위점역발생돌변.결론 귀양지구2010년하추계급성위장염이낙여병독GⅡ형위주,병차변이주구유다양성.
Objective To study the composition of variant and point mutations of Norovirus GⅡ.4 in Guiyang regions.Methods From June to November 2010,cases information and fecal specimens were collected from guard-hospitals in Guiyang regions,who had caught the acute-gastroenteritis.Noroviruses in specimens were detected by a real-time reverse transcription polymerase chain reaction(real-time RT-PCR),and then partial genotyped norovirus-positive clinical samples (in random) were cloned and sequenced in VP1 gene code.Furthermore,the gene sequences were compared with the published variants at home and abroad of norovirus(GⅡ.4),including the phylogenetic analyses of genomes and variation of amino acids within individual sites.Results Those 267 specimens were GⅡ-norovirus-positive(62.68％) in 426 clinical samples.There were nine GⅡ.4-norovirus-positive VP1 gene-sequences available,and two subtype-norovirus variants (GⅡ.4 2008a and G Ⅱ.4 2008b variant) were epidemic in 2010,Guizhou province.The homology between and in subgroups were 95.90％-96.72％ and 99.45％-100％.Two amino acids within individual sites were apt to mutate.Conclusion Norovirus GⅡ genotype were predominant in summer and fall acute gastroenteritis in 2010 for Guiyang regions,and the variants were diversity.