中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
7期
636-641
,共6页
乙型肝炎病毒%肝衰竭%自然杀伤细胞
乙型肝炎病毒%肝衰竭%自然殺傷細胞
을형간염병독%간쇠갈%자연살상세포
Hepatitis B virus%Liver failure%Natural killer cell
目的 探讨HBV相关慢加急性肝衰竭(HBV-ACLF)患者外周血和肝组织自然杀伤(NK)细胞的表达频率及功能与肝损伤的相关性.方法 利用多色流式技术检测14例HBV-ACLF患者和15例HBV-CHB(慢性乙型肝炎)患者外周血NK细胞(CD3-CD56+)的表达频率和NK细胞表面CD107a的表达,胞内细胞因子染色技术检测NK细胞分泌IFN-γ的水平.收集20例HBV-ACLF患者、10例轻度CHB患者肝组织穿刺标本,利用免疫组织化学双染技术检测肝组织中CD3和CD57抗原的表达.分析轻度CHB和HBV-ACLF患者肝脏NK细胞的表达频率及与肝损伤程度(TBIL水平)的相关性.结果 HBV-ACLF患者与轻度CHB患者外周血NK细胞的表达频率和IFN-γ的表达差异均无统计学意义(12.7％ ±5.3％ vs 9.6％±3.3％和4.52％ ±2.52％ vs 9.06％±7.6％,P均＞0.05),HBV-ACLF患者外周血CD107a表达(NK细胞的杀伤活性)要显著强于轻度CHB患者(52.1％±18.9％ vs 30.9％ ±12.4％,P＜0.05).HBV-ACLF患者肝组织中CD57+NK细胞的表达频率显著高于轻度CHB患者(95.1±21.3/低倍镜视野 vs 9.5 ±10.6/低倍镜视野,P＜0.01).肝脏CD57+NK细胞的表达频率与TBIL水平显著正相关,相关系数为0.936.结论 外周血NK细胞杀伤活性显著增强和肝脏中CD57+NK细胞大量募集可能是导致HBV-ACLF免疫性肝损伤加剧,肝细胞大量凋亡和坏死的重要因素.
目的 探討HBV相關慢加急性肝衰竭(HBV-ACLF)患者外週血和肝組織自然殺傷(NK)細胞的錶達頻率及功能與肝損傷的相關性.方法 利用多色流式技術檢測14例HBV-ACLF患者和15例HBV-CHB(慢性乙型肝炎)患者外週血NK細胞(CD3-CD56+)的錶達頻率和NK細胞錶麵CD107a的錶達,胞內細胞因子染色技術檢測NK細胞分泌IFN-γ的水平.收集20例HBV-ACLF患者、10例輕度CHB患者肝組織穿刺標本,利用免疫組織化學雙染技術檢測肝組織中CD3和CD57抗原的錶達.分析輕度CHB和HBV-ACLF患者肝髒NK細胞的錶達頻率及與肝損傷程度(TBIL水平)的相關性.結果 HBV-ACLF患者與輕度CHB患者外週血NK細胞的錶達頻率和IFN-γ的錶達差異均無統計學意義(12.7％ ±5.3％ vs 9.6％±3.3％和4.52％ ±2.52％ vs 9.06％±7.6％,P均＞0.05),HBV-ACLF患者外週血CD107a錶達(NK細胞的殺傷活性)要顯著彊于輕度CHB患者(52.1％±18.9％ vs 30.9％ ±12.4％,P＜0.05).HBV-ACLF患者肝組織中CD57+NK細胞的錶達頻率顯著高于輕度CHB患者(95.1±21.3/低倍鏡視野 vs 9.5 ±10.6/低倍鏡視野,P＜0.01).肝髒CD57+NK細胞的錶達頻率與TBIL水平顯著正相關,相關繫數為0.936.結論 外週血NK細胞殺傷活性顯著增彊和肝髒中CD57+NK細胞大量募集可能是導緻HBV-ACLF免疫性肝損傷加劇,肝細胞大量凋亡和壞死的重要因素.
목적 탐토HBV상관만가급성간쇠갈(HBV-ACLF)환자외주혈화간조직자연살상(NK)세포적표체빈솔급공능여간손상적상관성.방법 이용다색류식기술검측14례HBV-ACLF환자화15례HBV-CHB(만성을형간염)환자외주혈NK세포(CD3-CD56+)적표체빈솔화NK세포표면CD107a적표체,포내세포인자염색기술검측NK세포분비IFN-γ적수평.수집20례HBV-ACLF환자、10례경도CHB환자간조직천자표본,이용면역조직화학쌍염기술검측간조직중CD3화CD57항원적표체.분석경도CHB화HBV-ACLF환자간장NK세포적표체빈솔급여간손상정도(TBIL수평)적상관성.결과 HBV-ACLF환자여경도CHB환자외주혈NK세포적표체빈솔화IFN-γ적표체차이균무통계학의의(12.7％ ±5.3％ vs 9.6％±3.3％화4.52％ ±2.52％ vs 9.06％±7.6％,P균＞0.05),HBV-ACLF환자외주혈CD107a표체(NK세포적살상활성)요현저강우경도CHB환자(52.1％±18.9％ vs 30.9％ ±12.4％,P＜0.05).HBV-ACLF환자간조직중CD57+NK세포적표체빈솔현저고우경도CHB환자(95.1±21.3/저배경시야 vs 9.5 ±10.6/저배경시야,P＜0.01).간장CD57+NK세포적표체빈솔여TBIL수평현저정상관,상관계수위0.936.결론 외주혈NK세포살상활성현저증강화간장중CD57+NK세포대량모집가능시도치HBV-ACLF면역성간손상가극,간세포대량조망화배사적중요인소.
Objective To investigate whether the expression frequency and function of peripheral and liver NK cells was correlated with the liver injury in patients with hepatitis B virus related acute-on-chronic liver failure(HBV-ACLF).Methods Peripheral blood samples were obtained from fifteen HBV-ACLF patients and fifteen chronic hepatitis B patients.The frequency of peripheral NK cells (CD3-CD56+) and CD107a expression on surface of peripheral NK cells were detected by multicolor flow cytometry.Expression of IFN-γ by peripheral NK cells was detected by intracellular cytokine staining.Needle biopsy liver tissues were obtained from twenty patients with HBV-ACLF,ten patients with mild CHB,and expression of live NK cells (CD3-CD57+) was analyzed by dual immmunohistochemical staining of CD 3 and CD57.Results There was no significant difference in the frequency of peripheral NK cell and IFN-γ expression by peripheral NK cells between HBV-ACLF and mild CHB patients.However,CD107a expression on surface of peripheral NK cells of HBV-ACLF patients was remarkably higher than that of CHB patients.The frequency of liver CD57+NK cell of HBV-ACLF patients was remarkably higher than that of CHB patients(95.1 ±21.3/low power field vs 9.5±10.6/low power field,P＜0.01).Further analysis revealed that the frequency of liver CD57+ NK cells in HBV-ACLF patients was positively correlated with the TBIL level.Conclusion The enhanced cytotoxic activity of peripheral NK cells and the recruitment of liver CD57+ NK cells may aggravate immune-mediated liver injury and promote the apoptosis and necrosis of hepatocytes.
