中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
8期
685-688
,共4页
张传兴%王璐%张毅%朱传福
張傳興%王璐%張毅%硃傳福
장전흥%왕로%장의%주전복
人类白细胞抗原%多态性%汉族
人類白細胞抗原%多態性%漢族
인류백세포항원%다태성%한족
Human leukocyte antigen%Polymorphism%Han population
目的 分析济南地区汉族人群人类白细胞抗原(human leukocyte antigens,HLA)-A、B、DRB1座位等位基因的高分辨多态性.方法 采用PCR-测序分型(PCR-sequence-based typing,PCR-SBT)对鲁南地区483名无血缘关系的汉族健康个体进行HLA-A、B、DRB1座位高分辨基因分型,采用Arlequin3.5软件计算等位基因频率、单倍型频率,并就常见等位基因与其他人群进行比较.结果 济南汉族HLA-A、B、DRB1座位分别检出27、56和41个等位基因,其中等位基因频率分布最高的分别是A* 11∶01 (0.1615)、B*13∶02(0.1163)和DRB1* 07∶01 (0.1763)；最常见的A*-B*-DRB1*单倍型是A*30∶01-B* 13∶02-DRB1* 07∶01 (0.0867).结论 济南地区汉族人群HLA-A、B、DRB1等位基因和单倍型具有较高的多态性.
目的 分析濟南地區漢族人群人類白細胞抗原(human leukocyte antigens,HLA)-A、B、DRB1座位等位基因的高分辨多態性.方法 採用PCR-測序分型(PCR-sequence-based typing,PCR-SBT)對魯南地區483名無血緣關繫的漢族健康箇體進行HLA-A、B、DRB1座位高分辨基因分型,採用Arlequin3.5軟件計算等位基因頻率、單倍型頻率,併就常見等位基因與其他人群進行比較.結果 濟南漢族HLA-A、B、DRB1座位分彆檢齣27、56和41箇等位基因,其中等位基因頻率分佈最高的分彆是A* 11∶01 (0.1615)、B*13∶02(0.1163)和DRB1* 07∶01 (0.1763)；最常見的A*-B*-DRB1*單倍型是A*30∶01-B* 13∶02-DRB1* 07∶01 (0.0867).結論 濟南地區漢族人群HLA-A、B、DRB1等位基因和單倍型具有較高的多態性.
목적 분석제남지구한족인군인류백세포항원(human leukocyte antigens,HLA)-A、B、DRB1좌위등위기인적고분변다태성.방법 채용PCR-측서분형(PCR-sequence-based typing,PCR-SBT)대로남지구483명무혈연관계적한족건강개체진행HLA-A、B、DRB1좌위고분변기인분형,채용Arlequin3.5연건계산등위기인빈솔、단배형빈솔,병취상견등위기인여기타인군진행비교.결과 제남한족HLA-A、B、DRB1좌위분별검출27、56화41개등위기인,기중등위기인빈솔분포최고적분별시A* 11∶01 (0.1615)、B*13∶02(0.1163)화DRB1* 07∶01 (0.1763)；최상견적A*-B*-DRB1*단배형시A*30∶01-B* 13∶02-DRB1* 07∶01 (0.0867).결론 제남지구한족인군HLA-A、B、DRB1등위기인화단배형구유교고적다태성.
Objective To analyze the polymorphic character of HLA-A,B,DRB1 alleles at highresolution level in Ji-nan Han population.Methods A total of 483 unrelated and healthy Han ethnic individual from Ji-nan region were genotyped by PCR-SBT for HLA-A,B and DRB1 loci.Then allelic and haplotypic frequencies were calculated by maximum likelihood estimation method using Arlequin software.Results There were 27,56 and 41 alleles identified at HLA-A,B and DRB1 loci respectively,and the most common alleles were A * 11∶01(0.1615),B * 13∶02(0.1163) and DRB1 * 07∶01 (0.1763).The three loci haplotype was dominated by A* 30∶01-B * 13∶02-DRB1 * 07∶01 with a frequency of 0.0867.Conclusion The HLA-A,B and DRB1 loci are highly polymorphic in Ji-nan Han population.
