CAJ | 학술논문

目的探究人巨细胞病毒(HCMV) pp65是否可以诱发正常C57BL/6小鼠产生系统性红斑狼疮(SLE)相关实验室诊断指标的变化.方法构建HCMV pp65原核表达质粒与真核表达质粒,然后进行HCMV pp65原核蛋白表达与纯化.间接法ELISA检测anti-pp65 IgG、dsDNA、抗核抗体(ANA),竞争法ELISA检测血浆中IL-1b、IL-6、TNF-α浓度.结果成功获得HCMV pp65原核表达蛋白和真核表达质粒,免疫小鼠后血清中anti-pp65、抗dsDNA抗体、ANA、IL-6均有明显上升趋势.结论 HCMVpp65可以使C57BL/6小鼠SLE相关检测指标发生明显改变,这一结果有助于进一步研究自身免疫病的发病机制与影响因素.
목적 탐구인거세포병독(HCMV) pp65시부가이유발정상C57BL/6소서산생계통성홍반랑창(SLE)상관실험실진단지표적변화.방법 구건HCMV pp65원핵표체질립여진핵표체질립,연후진행HCMV pp65원핵단백표체여순화.간접법ELISA검측anti-pp65 IgG、dsDNA、항핵항체(ANA),경쟁법ELISA검측혈장중IL-1b、IL-6、TNF-α농도.결과 성공획득HCMV pp65원핵표체단백화진핵표체질립,면역소서후혈청중anti-pp65、항dsDNA항체、ANA、IL-6균유명현상승추세.결론 HCMVpp65가이사C57BL/6소서SLE상관검측지표발생명현개변,저일결과유조우진일보연구자신면역병적발병궤제여영향인소.
Objective To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV pp65) in murine systemic lupus erythematosus (SLE).Methods The prokaryotic vector pET-28b and eukaryotic vector pcDNA 3.0 were constructed to express the HCMV pp65 protein.All the C57BL/6 mice were inoculated with pp65 eukaryotic vector intramuscularly five times at 2-week intervals and then were bled via the retro-orbital vein.Subsequently,indirect ELISA was used to evaluate the concentration of anti-pp65 IgG,anti-dsDNA and ANA.At the same time,IL-1 b,IL-6,and TNF-α were determined by competitive ELISA.Results The early onset of autoantibodies and an overexpression of IL-6 were observed in immunized male C57BL/6 mice.Conclusion HCMV pp65 triggers the deregulation of humoral immunity in C57BL/6 mice,which indicates that the immune responses induced by HCMV pp65 may be involved in the development of SLE.