CAJ | 학술논문

目的了解并确定2002-2008年杭州地区伤寒沙门菌、甲型副伤寒沙门菌优势菌株的分子特征.方法采用脉冲场凝胶电泳(PFGE)、多位点串联重复序列分析(MLVA)或多位点序列分型(MLST)对2002-2008年杭州地区分离的31株伤寒沙门菌、404株甲型副伤寒沙门菌进行分型并分析.结果 404株甲型副伤寒沙门菌可分为6个PFGE型,P1型和P2型属于同一个克隆系,99.0％ (400/404)菌株属于该克隆系,其中P1型菌株占该克隆系菌株的93.3％ (373/400).31株伤寒沙门菌株存在高度多样性,可分为14个PFGE型、28个MLVA型(分辨率90.3％)、3个MIST型.根据MLVA各型靶位点多态性差异,本地区流行的伤寒沙门菌株与东南亚地区菌株相近,但与欧洲菌株差距较大,呈高度多态性的可变串联重复序列(VNTR)位点TR1、TR2、Sal02可作为本地区伤寒沙门菌株的检测标志.伤寒沙门菌株MLST型别包括了目前国际上发现的所有3个型别,但以ST2型为主(23/31,74.2％).结论近年杭州地区甲型副伤寒疫情由同一亚系菌株感染所致,但本地区流行的伤寒沙门菌株呈现高度多样性.
목적 료해병학정2002-2008년항주지구상한사문균、갑형부상한사문균우세균주적분자특정.방법 채용맥충장응효전영(PFGE)、다위점천련중복서렬분석(MLVA)혹다위점서렬분형(MLST)대2002-2008년항주지구분리적31주상한사문균、404주갑형부상한사문균진행분형병분석.결과 404주갑형부상한사문균가분위6개PFGE형,P1형화P2형속우동일개극륭계,99.0％ (400/404)균주속우해극륭계,기중P1형균주점해극륭계균주적93.3％ (373/400).31주상한사문균주존재고도다양성,가분위14개PFGE형、28개MLVA형(분변솔90.3％)、3개MIST형.근거MLVA각형파위점다태성차이,본지구류행적상한사문균주여동남아지구균주상근,단여구주균주차거교대,정고도다태성적가변천련중복서렬(VNTR)위점TR1、TR2、Sal02가작위본지구상한사문균주적검측표지.상한사문균주MLST형별포괄료목전국제상발현적소유3개형별,단이ST2형위주(23/31,74.2％).결론 근년항주지구갑형부상한역정유동일아계균주감염소치,단본지구류행적상한사문균주정현고도다양성.
Objective To determine the molecular characteristics of predominant Salmonella typhi and Salmonella paratyphi A strains prevalent in Hangzhou area from 2002 to 2008.Methods Pulse field gel electrophoresis (PFGE),multi-locus variable-number tandem repeat analysis (MLVA) and multi-locus sequence typing (MLST) were applied for typing as well as analysis of the molecular characteristics of 31 S.typhi isolates and 404 S.paratyphi A isolates from Hangzhou area during 2002 to 2008.Results The 404 S.paratyphi A isolates could be divided into six PFGE types (P1-P6).99.0％ of the S.paratyphi A isolates (400/404) belonged to the same one clone family (P1 and P2 types),in which P1 strains occupied 93.3％ (373/400) of the isolates.The 31 S.typhi isolates displayed a high diversity,which could be classified into 14 PFGE types,28 MLVA types with 90.3％ resolving power and 3 MLST types.The S.typhi strains prevalent in Hangzhou area were similar to those in Southeast Asia but different from those in Europe.The variable number tandem repeat (VNTR) sites with high polymorphism,TR1,TR2 and Sal02,could be used to the markers for diagnosis of S.typhi isolates in the area.The MLST types of 31 S.typhi isolates included all the three types currently found in the world but the ST2 type of S.typhi strains was predominant (23/31,74.2％).Conclusion The paratyphoid A prevalence in Hangzhou area in the recent years is caused by infection of the same clone family of S.paratyphi A whereas the S.typhi strains prevalent in the area display a high diversity.