中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
9期
808-812
,共5页
董敖%陶然%胡颖姿%华春珍%尚世强
董敖%陶然%鬍穎姿%華春珍%尚世彊
동오%도연%호영자%화춘진%상세강
甘露(聚)糖结合凝集素%基因多态%儿童%感染
甘露(聚)糖結閤凝集素%基因多態%兒童%感染
감로(취)당결합응집소%기인다태%인동%감염
Mannose/mannan-binding lectin%Genetic polymorphism%Children%Infection
目的 研究浙江省汉族儿童中甘露糖结合凝集素(MBL)基因多态性和蛋白水平与常见感染(反复呼吸道感染、急性呼吸道感染、CMV活动性感染、体表局部脓肿、中耳炎)易感性的关系.方法 用PCR和测序法对感染组和对照组儿童的MBL基因启动子区和外显子1区的6个突变位点进行检测和分型,用ELISA试剂盒检测两组儿童的血浆MBL蛋白浓度.结果 感染组和对照组均未检测到MBL基因外显子1区+223位点(C/T)和+239位点(G/A)的突变,对照组也未检测到启动子区+4位点(C/T)的突变.启动子区-550位点3种基因型HH、HL、LL在感染组和对照组的频率分别为43.1％、29.1％、27.8％和63.8％、17.1％、19.1％,两组间基因型频率差异有统计学意义(P＜0.05).启动子区和外显子1区所有基因型组合形成的完整基因型可分为与血浆蛋白浓度相关的“YA型”和“XB型”,两种完整基因型频率在感染组和对照组间差异有统计学意义(P＜0.05).感染组和对照组血浆MBL蛋白浓度均呈偏态分布.CMV感染组蛋白浓度低于对照组,急性呼吸道感染和局部脓肿组蛋白浓度高于对照组,差异均有统计学意义(P＜0.05).结论 包括启动子区和外显子1区在内的MBL基因多态性与儿童常见感染的易感性具有一定相关性.
目的 研究浙江省漢族兒童中甘露糖結閤凝集素(MBL)基因多態性和蛋白水平與常見感染(反複呼吸道感染、急性呼吸道感染、CMV活動性感染、體錶跼部膿腫、中耳炎)易感性的關繫.方法 用PCR和測序法對感染組和對照組兒童的MBL基因啟動子區和外顯子1區的6箇突變位點進行檢測和分型,用ELISA試劑盒檢測兩組兒童的血漿MBL蛋白濃度.結果 感染組和對照組均未檢測到MBL基因外顯子1區+223位點(C/T)和+239位點(G/A)的突變,對照組也未檢測到啟動子區+4位點(C/T)的突變.啟動子區-550位點3種基因型HH、HL、LL在感染組和對照組的頻率分彆為43.1％、29.1％、27.8％和63.8％、17.1％、19.1％,兩組間基因型頻率差異有統計學意義(P＜0.05).啟動子區和外顯子1區所有基因型組閤形成的完整基因型可分為與血漿蛋白濃度相關的“YA型”和“XB型”,兩種完整基因型頻率在感染組和對照組間差異有統計學意義(P＜0.05).感染組和對照組血漿MBL蛋白濃度均呈偏態分佈.CMV感染組蛋白濃度低于對照組,急性呼吸道感染和跼部膿腫組蛋白濃度高于對照組,差異均有統計學意義(P＜0.05).結論 包括啟動子區和外顯子1區在內的MBL基因多態性與兒童常見感染的易感性具有一定相關性.
목적 연구절강성한족인동중감로당결합응집소(MBL)기인다태성화단백수평여상견감염(반복호흡도감염、급성호흡도감염、CMV활동성감염、체표국부농종、중이염)역감성적관계.방법 용PCR화측서법대감염조화대조조인동적MBL기인계동자구화외현자1구적6개돌변위점진행검측화분형,용ELISA시제합검측량조인동적혈장MBL단백농도.결과 감염조화대조조균미검측도MBL기인외현자1구+223위점(C/T)화+239위점(G/A)적돌변,대조조야미검측도계동자구+4위점(C/T)적돌변.계동자구-550위점3충기인형HH、HL、LL재감염조화대조조적빈솔분별위43.1％、29.1％、27.8％화63.8％、17.1％、19.1％,량조간기인형빈솔차이유통계학의의(P＜0.05).계동자구화외현자1구소유기인형조합형성적완정기인형가분위여혈장단백농도상관적“YA형”화“XB형”,량충완정기인형빈솔재감염조화대조조간차이유통계학의의(P＜0.05).감염조화대조조혈장MBL단백농도균정편태분포.CMV감염조단백농도저우대조조,급성호흡도감염화국부농종조단백농도고우대조조,차이균유통계학의의(P＜0.05).결론 포괄계동자구화외현자1구재내적MBL기인다태성여인동상견감염적역감성구유일정상관성.
Objective To investigate the association between genetic polymorphisms and protein levels of mannose-binding lectin (MBL) and the sensitivities of common infections in a pediatric Han population lived in Zhejiang Province.Methods MBL genetic polymorphisms of patients and controls were detected by PCR-based sequencing.MBL protein levels were measured using MBL ELISA Kit.Results No mutations at positions +223 and +239 of the exon 1 were detected in either patients or controls.No mutation at position +4 of the promoter was detected in controls.The frequencies of the three genotypes HH,HL,and LL at position-550 of the promoter were different between patients and controls(P＜0.05).The frequencies of genotypes "YA" and "XB" relevant to MBL protein levels were also different between patients and controls(P＜0.05).Comparing the frequencies of genotypes "YA" and "XB" in separate infectious disease with controls,significant differences were found in the group of RRI and CMV infection.The distributions of serum MBL level frequencies in patients and controls were both characterized by skewed distributions.MBL levels of patients with CMV infection were lower than those of controls(P＜0.05).Inversely,MBL levels of patients with acute respiratory infection and localized abscess were higher than those of controls (P＜O.05).Conclusion Genetic polymorphism of MBL gene is seemed to be relative to the sensitivity of common infections in children.
