中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
9期
813-815
,共3页
李靖%何艳%郑煜煌%刘艳科%尹柯%封文军%杨晓云%刘超群%何芳%屈满英%欧阳成林
李靖%何豔%鄭煜煌%劉豔科%尹柯%封文軍%楊曉雲%劉超群%何芳%屈滿英%歐暘成林
리정%하염%정욱황%류염과%윤가%봉문군%양효운%류초군%하방%굴만영%구양성림
肺结核%Th17细胞%调节性T细胞%抗结核治疗
肺結覈%Th17細胞%調節性T細胞%抗結覈治療
폐결핵%Th17세포%조절성T세포%항결핵치료
Tuberculosis%Th17 cell%Regulatory T cell (Tr)%Anti-tuberculosis
目的 观察肺结核患者CD3+ CD4+ IL-17+T细胞(Th17细胞)和CD4+ CD25+ Foxp3+T 细胞(调节性T细胞,Tr)的平衡状态及其在6个月抗结核治疗中的变化.方法 选取经抗结核治疗的肺结核患者32例,同时选取32例健康志愿者为对照,分别于治疗0、3、6个月采集静脉血,用流式检测Th17细胞及Tr细胞百分比,并对比分析其相互关系.结果 肺结核患者在抗结核治疗前、治疗3月、6个月与正常人外周血的Th17细胞在CD4+T细胞中的比例分别为(1.10±0.39)％、(2.50±1.03)％、(3.90±1.34)％、(4.50±1.52)％；Tr细胞在CD4+T细胞中的比例分别为(9.17±3.26)％、(6.85±2.73)％、(5.46±1.69)％、(4.35±0.86)％.结论 结核分枝杆菌感染导致Th17细胞和Tr细胞的失平衡,而抗结核治疗可能逐渐恢复二者的免疫平衡状态.免疫细胞量的变化有可能成为观察肺结核进展和抗结核疗效的参考指标.
目的 觀察肺結覈患者CD3+ CD4+ IL-17+T細胞(Th17細胞)和CD4+ CD25+ Foxp3+T 細胞(調節性T細胞,Tr)的平衡狀態及其在6箇月抗結覈治療中的變化.方法 選取經抗結覈治療的肺結覈患者32例,同時選取32例健康誌願者為對照,分彆于治療0、3、6箇月採集靜脈血,用流式檢測Th17細胞及Tr細胞百分比,併對比分析其相互關繫.結果 肺結覈患者在抗結覈治療前、治療3月、6箇月與正常人外週血的Th17細胞在CD4+T細胞中的比例分彆為(1.10±0.39)％、(2.50±1.03)％、(3.90±1.34)％、(4.50±1.52)％；Tr細胞在CD4+T細胞中的比例分彆為(9.17±3.26)％、(6.85±2.73)％、(5.46±1.69)％、(4.35±0.86)％.結論 結覈分枝桿菌感染導緻Th17細胞和Tr細胞的失平衡,而抗結覈治療可能逐漸恢複二者的免疫平衡狀態.免疫細胞量的變化有可能成為觀察肺結覈進展和抗結覈療效的參攷指標.
목적 관찰폐결핵환자CD3+ CD4+ IL-17+T세포(Th17세포)화CD4+ CD25+ Foxp3+T 세포(조절성T세포,Tr)적평형상태급기재6개월항결핵치료중적변화.방법 선취경항결핵치료적폐결핵환자32례,동시선취32례건강지원자위대조,분별우치료0、3、6개월채집정맥혈,용류식검측Th17세포급Tr세포백분비,병대비분석기상호관계.결과 폐결핵환자재항결핵치료전、치료3월、6개월여정상인외주혈적Th17세포재CD4+T세포중적비례분별위(1.10±0.39)％、(2.50±1.03)％、(3.90±1.34)％、(4.50±1.52)％；Tr세포재CD4+T세포중적비례분별위(9.17±3.26)％、(6.85±2.73)％、(5.46±1.69)％、(4.35±0.86)％.결론 결핵분지간균감염도치Th17세포화Tr세포적실평형,이항결핵치료가능축점회복이자적면역평형상태.면역세포량적변화유가능성위관찰폐결핵진전화항결핵료효적삼고지표.
Objective To observe the Th17 and regulatory T cells(Tr) equilibrium state as well as their changes of tuberculosis patients in six-month's anti-Tuberculosis treatment.Methods Select thirty-two tuberculosis patients received anti-Tuberculosis treatment while thirty-two healthy volunteers as controls.Flow cytometry was used to analyze Th17 and Tr cells in venous blood at the time of pre-therapy,3th,6th month.Results The ratio of Th17 cells in CD4 cells in tuberculosis patients and volunteers were (1.10±0.39)％,(2.50±1.03) ％,(3.90±1.34) ％,(4.50±1.52)％,respectively; the ratio of Tr cells were (9.17±3.26)％,(6.85±2.73)％,(5.46±1.69)％,(4.35±0.86)％,respectively.Conclusion Tuberculosis could make Th17 cells and Tr cells lost their balance,but the immune equilibrium state may gradually recover after anti-tuberculosis.The change of the amount of immune cells was likely to be the reference indexes to observe the progress of tuberculosis and the treatment effect of anti-tuberculosis.
