CAJ | 학술논문

目的探讨二硫代氨基甲酸吡咯烷(PDTC)对PV-杀白细胞素(panton-valentine Ieucocidin,PVL)诱导THP-1巨噬细胞NF-κB及炎性因子表达的影响.方法实验分3组,PBS对照组、rPVL刺激组和PDTC处理组,在加入rPVL刺激前60 min,给予100 μmol/L PDTC预处理THP-1巨噬细胞.采用免疫组化检测NF-κB的移位,Western blot检测细胞胞质IκB蛋白和胞核NF-κB蛋白表达,ELISA法检测细胞上清IL-8和IL-6蛋白的表达,RT-PCR法检测IL-8和IL-6 mRNA水平表达.结果 PDTC处理组NF-κB阳性细胞明显减少,核蛋白表达降低,胞质IκB蛋白降解减少；IL-8和IL-6mRNA表达明显下调,蛋白分泌量分别由12.96 ng/ml和6.55 ng/ml降低到6.78 ng/ml和3.88ng/ml,差异有统计学意义(P＜0.05).结论 PDTC可能通过抑制NF-κB的活性,从而降低II-8和IL-6的表达,对PVL引起的炎性损伤具有一定的保护作用.
목적 탐토이류대안기갑산필각완(PDTC)대PV-살백세포소(panton-valentine Ieucocidin,PVL)유도THP-1거서세포NF-κB급염성인자표체적영향.방법 실험분3조,PBS대조조、rPVL자격조화PDTC처리조,재가입rPVL자격전60 min,급여100 μmol/L PDTC예처리THP-1거서세포.채용면역조화검측NF-κB적이위,Western blot검측세포포질IκB단백화포핵NF-κB단백표체,ELISA법검측세포상청IL-8화IL-6단백적표체,RT-PCR법검측IL-8화IL-6 mRNA수평표체.결과 PDTC처리조NF-κB양성세포명현감소,핵단백표체강저,포질IκB단백강해감소；IL-8화IL-6mRNA표체명현하조,단백분비량분별유12.96 ng/ml화6.55 ng/ml강저도6.78 ng/ml화3.88ng/ml,차이유통계학의의(P＜0.05).결론 PDTC가능통과억제NF-κB적활성,종이강저II-8화IL-6적표체,대PVL인기적염성손상구유일정적보호작용.
Objective To investigate effect of PDTC on the NF-κB activation and the expression of inflammatory cytokines in THP-1 macrophages induced by rPVL.Methods The study was divided into three groups:PBS-treated control group,rPVL-treated group and PDTC group which was given 100 μmol/L PDTC at 60 min before rPVL exposure.Immunohistochemistry method was used to test the translocation of NF-κB protein; the expression of NF-κB and IκB protein was analyzed by Western blot; RT-PCR and ELISA was performed to test expression of IL-8 and L-6 in THP-1 macrophages.Results Compared with rPVL-treated group,the activation of NF-κB and the expression of IL-8 and L-6 in PDTC group was significantly decreased.The protein secretions of IL-8 and IL-6 were reduced to 6.78 ng/ml,3.88 ng/ml,receptively(P ＜0.05).Conclusion The inhibitor of NF-κB,PDTC,could significantly decrease the secretion of pro-inflammatory in THP-1 macrophages by rPVL,and it suggested that PDTC played an important role in protecting tissues from damage induced by rPVL.