CAJ | 학술논문

目的研究5型腺病毒(Ad5)不同途径感染小鼠后体内代谢和组织分布特点,为Ad5载体疫苗的应用和改造提供参考.方法构建携带萤火虫荧光素酶基因的Ad5-Fluc病毒并利用活体成像技术分析其感染BALB/c小鼠后的蛋白表达和组织分布特点,以及与裸鼠的感染特点进行比较.结果 Ad5-Fluc肌肉途径感染BALB/c小鼠后出现肝脾转移表达的特点,且肌肉途径表达周期最长(＞60d),表达水平最高(P＜0.01).病毒感染裸鼠后分布特点与BALB/c小鼠一致但表达周期明显延长.另外,用Ad35的纤突蛋白替代Ad5的纤突蛋白后的5型腺病毒载体(Ad5F35)在保持相似表达效率的同时消除了肝嗜性的特点.结论肌肉途径可能为5型腺病毒载体疫苗理想的免疫方式,同时Ad5F35病毒是一种理想的替代性腺病毒疫苗载体.
목적 연구5형선병독(Ad5)불동도경감염소서후체내대사화조직분포특점,위Ad5재체역묘적응용화개조제공삼고.방법 구건휴대형화충형광소매기인적Ad5-Fluc병독병이용활체성상기술분석기감염BALB/c소서후적단백표체화조직분포특점,이급여라서적감염특점진행비교.결과 Ad5-Fluc기육도경감염BALB/c소서후출현간비전이표체적특점,차기육도경표체주기최장(＞60d),표체수평최고(P＜0.01).병독감염라서후분포특점여BALB/c소서일치단표체주기명현연장.령외,용Ad35적섬돌단백체대Ad5적섬돌단백후적5형선병독재체(Ad5F35)재보지상사표체효솔적동시소제료간기성적특점.결론 기육도경가능위5형선병독재체역묘이상적면역방식,동시Ad5F35병독시일충이상적체대성선병독역묘재체.
Objective To study the in vivo expression and biodistribution of Ad5-Fluc (Adenovirus carrying firefly luciferase genes) in mice.Methods The recombinant Ad5-Fluc virus was constructed and infected to BALB/c or nude mice through three different routes.The protein expression level,tissue distribution and the characteristics of infection were analyzed by in vivo bioluminescence imaging technology.Results Compared to other two routes,the BALB/c mice infected through muscular route had the longest expression cycle (over 60 days) and the highest expression level,while the virus was transferred into the liver and spleen after infection.The nude mice had a significantly extended expression cycle than BALB/c mice.Moreover,the characteristic of liver tropism was eliminated after Ad5 F35 infection in mice,while maintained similar expression efficiency.Conclusion Due to the highest expression efficiency,the muscular route would be the optimal route for Ad5 vector based vaccination.In addition,Ad5F35 virus could become an ideal alternative vaccine vector for eliminating the liver tropism.