CAJ | 학술논문

目的研究西藏地区水痘-带状疱疹病毒临床分离株基因型.方法将临床采集的水痘或带状疱疹患者皮肤水疱液接种至人胚胎成纤维细胞进行病毒分离；对分离得到的病毒运用间接免疫荧光法鉴定,鉴定结果为阳性的病毒继续体外培养以提取其基因组DNA.PCR法扩增并测序水痘-带状疱疹病毒基因组中开放读码框1、21、50、54的部分片段.测序结果与GenBank中水痘-带状疱疹病毒参考株Dumas进行序列比对分析其中单核苷酸多态性,并利用Primer 5.0软件进行酶切位点分析,从而确定基因型.结果从临床采集的16份标本中经分离鉴定后得到10株水痘-带状疱疹病毒临床分离株,病毒分离阳性率为62.5％.基因分析结果显示西藏地区10株水痘-带状疱疹病毒临床分离株中基因型A1有3株,基因型A2有4株,基因型J1有3株.结论西藏地区水痘-带状疱疹病毒基因型分布与西藏地区地理位置密切相关.
목적 연구서장지구수두-대상포진병독림상분리주기인형.방법 장림상채집적수두혹대상포진환자피부수포액접충지인배태성섬유세포진행병독분리；대분리득도적병독운용간접면역형광법감정,감정결과위양성적병독계속체외배양이제취기기인조DNA.PCR법확증병측서수두-대상포진병독기인조중개방독마광1、21、50、54적부분편단.측서결과여GenBank중수두-대상포진병독삼고주Dumas진행서렬비대분석기중단핵감산다태성,병이용Primer 5.0연건진행매절위점분석,종이학정기인형.결과 종림상채집적16빈표본중경분리감정후득도10주수두-대상포진병독림상분리주,병독분리양성솔위62.5％.기인분석결과현시서장지구10주수두-대상포진병독림상분리주중기인형A1유3주,기인형A2유4주,기인형J1유3주.결론 서장지구수두-대상포진병독기인형분포여서장지구지리위치밀절상관.
Objective To analyze the genotypes of clinical varicella-zoster virus(VZV) isolates in Tibet.Methods The samples of vesicular fluid from patients with chickenpox or zoster were collected to isolate VZV in human embryonic fibroblast cells.Furtherly,the isolated VZV were identified by indirect immunofluorescence assay and the positive ones were continuely cultured in vitro to get their genome DNAs.Then,PCR and nucleotide sequencing were performed on the part of open reading frames 1,21,50,54.To confirm the genotypes of isolated virus strains,the gene sequences obtained from PCR products were compared with that in GenBank of VZV Dumas strains,and their restriction enzyme sites were analyzed via Primer 5.0.Results Ten clinical isolates of VZV were obtained from sixteen specimens,the positive isolation rate was 62.5％.Gene analysis showed that among all the ten clinical isolates of VZV in Tibet,three strains are genotype A1,four are genotype A2 and the other three are genotype J1.Conclusion The VZV genotype distribution in Tibet was tightly related to their special geographic locations.