中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
11期
939-943
,共5页
孙红妹%薛冠华%闫超%冯燕玲%王丽琼%赵汉青%李少丽%曹玲
孫紅妹%薛冠華%閆超%馮燕玲%王麗瓊%趙漢青%李少麗%曹玲
손홍매%설관화%염초%풍연령%왕려경%조한청%리소려%조령
肺炎支原体%基因分型%多位点可变数目串联重复序列分析%P1-限制性片段长度多态性%M-P分型系统
肺炎支原體%基因分型%多位點可變數目串聯重複序列分析%P1-限製性片段長度多態性%M-P分型繫統
폐염지원체%기인분형%다위점가변수목천련중복서렬분석%P1-한제성편단장도다태성%M-P분형계통
Mycoplasma pneumoniae%Genotype%Multiple-locus variable-number tandem-repeat analysis (MLVA)%P1-restriction fragment length polymorphism analysis (P1-RFLP)%M-P typing system
目的 对肺炎支原体(Mp)的暴发流行进行监测.方法 采用real-time PCR法对北京地区呼吸道感染患儿进行检测；阳性标本进行M-P基因分型[M:多位点可变数目串联重复序列分析(MLVA)；P:P1-限制性片段长度多态性(P1-RFLP)]分析.结果 2010年1月-2012年5月446例呼吸道感染患儿中,69例经real-time PCR检测为阳性,2010、2011及2012年上半年的感染率分别为:11.69％、15.56％及20.00％；采用我们提出的新的M-P基因分型系统,69例标本可分为11个基因型,其中M43562P1和M53562P1为2010至2011年两个主要基因型；M33562P1和M63562P1在2012年有感染上升趋势.结论 在本次国际Mp流行中,北京地区2011年肺炎支原体感染率也有所增加,主要基因型为M43562P1和M53562P1；其中M43562与欧洲流行基因型相同,M53562与以色列流行基因型相同.M-P基因分型系统,可以很好地监测世界不同地区肺炎支原体流行情况.
目的 對肺炎支原體(Mp)的暴髮流行進行鑑測.方法 採用real-time PCR法對北京地區呼吸道感染患兒進行檢測；暘性標本進行M-P基因分型[M:多位點可變數目串聯重複序列分析(MLVA)；P:P1-限製性片段長度多態性(P1-RFLP)]分析.結果 2010年1月-2012年5月446例呼吸道感染患兒中,69例經real-time PCR檢測為暘性,2010、2011及2012年上半年的感染率分彆為:11.69％、15.56％及20.00％；採用我們提齣的新的M-P基因分型繫統,69例標本可分為11箇基因型,其中M43562P1和M53562P1為2010至2011年兩箇主要基因型；M33562P1和M63562P1在2012年有感染上升趨勢.結論 在本次國際Mp流行中,北京地區2011年肺炎支原體感染率也有所增加,主要基因型為M43562P1和M53562P1；其中M43562與歐洲流行基因型相同,M53562與以色列流行基因型相同.M-P基因分型繫統,可以很好地鑑測世界不同地區肺炎支原體流行情況.
목적 대폐염지원체(Mp)적폭발류행진행감측.방법 채용real-time PCR법대북경지구호흡도감염환인진행검측；양성표본진행M-P기인분형[M:다위점가변수목천련중복서렬분석(MLVA)；P:P1-한제성편단장도다태성(P1-RFLP)]분석.결과 2010년1월-2012년5월446례호흡도감염환인중,69례경real-time PCR검측위양성,2010、2011급2012년상반년적감염솔분별위:11.69％、15.56％급20.00％；채용아문제출적신적M-P기인분형계통,69례표본가분위11개기인형,기중M43562P1화M53562P1위2010지2011년량개주요기인형；M33562P1화M63562P1재2012년유감염상승추세.결론 재본차국제Mp류행중,북경지구2011년폐염지원체감염솔야유소증가,주요기인형위M43562P1화M53562P1；기중M43562여구주류행기인형상동,M53562여이색렬류행기인형상동.M-P기인분형계통,가이흔호지감측세계불동지구폐염지원체류행정황.
Objective To inspect the source of an outbreak with Mycoplasma pneumoniae (Mp).Methods We carried out real-time PCR to analyze specimens collected from pediatric patients in Beijing during January 2010 to May 2012,diagnosed as pneumonia or a respiratory infection according to clinical symptoms.These positive samples were analyzed by the M-P typing system(M:multiple-locus variable-number tandem-repeat analysis,MLVA; P:P1-restriction fragment length polymorphism analysis,P1-RFLP).Results Sixty-nine specimens were tested positive to Mp by the real-time PCR in 446 specimens from pediatric patients.The infection rate was 11.69％,15.56％ and 20.00％ respectively in 2010,2011 and the first half of 2012.According to the M-P system,11 distinct genotypes were identified from 69 positive specimens,M43562P1 and M53562P1 were the two main genotypes that showed an increasing trend from 2010 to 2011,and M33562P1 and M63562P1 showed an increasing trend from 2011 to 2012 in China.Conclusion During this international Mp epidemic,the infection rate of Mp was also increase in Beijing in 2011,and M43562P1 and M53562P1 were the two main genotypes.Among them,M43562 were consistent with pop genotypes in Europe,and M53562 were consistent with pop genotype in Israel.The M-P system would be valuable to monitor the epidemic of Mp in different countries in the world.
