CAJ | 학술논문

目的研究艾滋病痴呆综合征(ADC)患者体内不同部位HIV-1nef基因变异导致氨基酸位点的改变,为研究ADC发病机制提供思路.方法克隆1例ADC病例尸检标本的外周(脾)和中枢神经系统(基底核、额叶灰质、脑膜、颞叶)共5个部位HIV-1 nef基因并测序,利用Bioedit、MEGA4等生物学软件与HIV-1标准株HXB2 Nef氨基酸序列进行比对,分析氨基酸位点变异情况.结果该ADC患者Nef蛋白某些氨基酸位点发生改变,且外周组织与中枢神经系统间及中枢神经系统不同部位间的变异情况不同,某些变异为外周和中枢所共有,某些变异仅发生在外周脾,而某些变异仅发生于中枢神经系统.这些变异可能不同程度地改变Nef蛋白某些生物学活性及功能,进而影响到HIV-1病毒的复制、感染及凋亡等.结论 ADC患者体内的HIV-1 nef基因变异可导致Nef蛋白某些氨基酸位点改变,且可能对其生物学活性造成影响,这些变异是否与ADC的发病机制有关有待进一步研究.
목적 연구애자병치태종합정(ADC)환자체내불동부위HIV-1nef기인변이도치안기산위점적개변,위연구ADC발병궤제제공사로.방법 극륭1례ADC병례시검표본적외주(비)화중추신경계통(기저핵、액협회질、뇌막、섭협)공5개부위HIV-1 nef기인병측서,이용Bioedit、MEGA4등생물학연건여HIV-1표준주HXB2 Nef안기산서렬진행비대,분석안기산위점변이정황.결과 해ADC환자Nef단백모사안기산위점발생개변,차외주조직여중추신경계통간급중추신경계통불동부위간적변이정황불동,모사변이위외주화중추소공유,모사변이부발생재외주비,이모사변이부발생우중추신경계통.저사변이가능불동정도지개변Nef단백모사생물학활성급공능,진이영향도HIV-1병독적복제、감염급조망등.결론 ADC환자체내적HIV-1 nef기인변이가도치Nef단백모사안기산위점개변,차가능대기생물학활성조성영향,저사변이시부여ADC적발병궤제유관유대진일보연구.
Objective To study the mutations of HIV-1 Nef proteins at amino acid level in different sites of neural tissues derived from a patient with AIDS dementia complex (ADC) and the possible pathogenic mechanism for ADC.Methods The nefgenes of HIV-1 from spleen and different brain tissues (basal ganglia,frontal gray matter,meninges and temporal lobe) of a corpse with ADC were amplified by PCR and sequenced.All sequences were analyzed by Bioedit and MEGA4 software.The mutations of HIV-1 Nef proteins at aminoacid level were analyzed by comparing with the amino acid sequences of a HIV-1 standard strain HXB2.Results HIV-1 Nef proteins isolated from different tissues of the patient with ADC showed different mutations at amino acid sites.Some mutations were co-existed in tissues from spleen and brain,while others were only found in spleen or brain tissues.These mutations might affect the biological activity or function of Nef proteins at certain degree and then have an impact on the replication,infection or apoptosis of HIV-1 viruses.Conclusion The mutations of HIV-1 nef genes resulted in some amino acid changes in Nef proteins isolated from different tissues of the patient with ADC,which might affect their biological activities.However,the correlation between these mutations and the pathogenesis of ADC still needs further investigation.