CAJ | 학술논문

目的探讨犬食管支架良性增生性狭窄模型的建立方法.方法采用补片外固定法建立模型.按照正交设计表确定2种类型的支架与6种规格的聚四氟乙烯补片的不同组合,支架采用头端杯口状、尾端蘑菇状设计.模型采用比格犬,术后观察犬进食情况,8周后处死实验犬观察支架段食管黏膜增生情况.按照进食情况和大体标本组织增生情况,对动物模型效果进行评分.结果正交设计提供8类组合方式.3类组合模型失败:1只犬完全不能进食,行支架回收;1只犬支架吐出;1只犬术后1周死于补片与支架之间的食管缺血坏死.4类组合模型实现食管黏膜肉芽增生,但存在缺陷.1类组合模型术后实验犬正常进食,透视随访支架位置固定,8周后大体标本可见食管黏膜炎症反应和肉芽增生显著.8类组合间在模型效果评价上差异有统计学意义(F=14.7000,P=0.031).结论采用体质量10 ～ 12 kg比格犬,总长度50 mm、主体直径20 mm、头端外径30 mm、尾端外径25 mm、头尾长度10 mm的裸支架,长度60 mm、宽度15 mm的补片可以获得组织增生良好的动物模型.
목적 탐토견식관지가량성증생성협착모형적건립방법.방법 채용보편외고정법건립모형.안조정교설계표학정2충류형적지가여6충규격적취사불을희보편적불동조합,지가채용두단배구상、미단마고상설계.모형채용비격견,술후관찰견진식정황,8주후처사실험견관찰지가단식관점막증생정황.안조진식정황화대체표본조직증생정황,대동물모형효과진행평분.결과 정교설계제공8류조합방식.3류조합모형실패:1지견완전불능진식,행지가회수;1지견지가토출;1지견술후1주사우보편여지가지간적식관결혈배사.4류조합모형실현식관점막육아증생,단존재결함.1류조합모형술후실험견정상진식,투시수방지가위치고정,8주후대체표본가견식관점막염증반응화육아증생현저.8류조합간재모형효과평개상차이유통계학의의(F=14.7000,P=0.031).결론 채용체질량10 ～ 12 kg비격견,총장도50 mm、주체직경20 mm、두단외경30 mm、미단외경25 mm、두미장도10 mm적라지가,장도60 mm、관도15 mm적보편가이획득조직증생량호적동물모형.
Objective To establish and observe the canine model with esophageal stent implantation for further study of the benign stenosis caused by proliferation.Methods According to orthogonal design,different combinations of two stents and six polytetrafluoroethylene (PTFE) patches were confirmed.Stent was designed as cylinder with mushroom shape on both ends.Beagle dogs (weight 10-12 kg) were adopted and cervical segment of esophagus were dissected.After PTFE patch was encircled around the esophagus,stent was delivered under fluoroscopy.The main body of the stent was located in accordance with the patch.Eating condition and position of the stent were followed on week 1,2,4,6 and 8.Gross specimen was harvested at the end point,and the degree of tissue hyperplasia was evaluated.Each animal model was given a mark according to the eating condition and tissue hyperplasia.Results Eight combinations of stent and patch were provided with orthogonal design.Three models failed for the following reasons:unable to eat in one dog,stent disgorged out in another,and the third died from esophageal necrosis between stent and patch.Four models had obvious tissue hyperplasia on the segment of stent,and weight loss or stent dislocation were observed in each model.One model developed appropriate tissue hyperplasia with normal diet,and stent dislocation was not found during the follow-up.Significant difference was confirmed among 8 models (F =14.7000,P =0.031).Conclusion Animal model with appropriate tissue hyperplasia could be established with following elements:beagle dogs weight from 10 kg to 12 kg; stent 50 mm in length,20 mm in diameter,with top mushroom 10 mm in length,30 mm in diameter,and end mushroom 10 mm in length,25 mm in diameter; PTFE patch 60 mm in length,15 mm in width.