中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2013年
12期
845-848
,共4页
宋允娜%郑萍%肖军华%卢战军
宋允娜%鄭萍%肖軍華%盧戰軍
송윤나%정평%초군화%로전군
卵泡抑素相关蛋白质类%结肠炎,溃疡性%酶联免疫吸附测定%免疫组织化学%染色与标记
卵泡抑素相關蛋白質類%結腸炎,潰瘍性%酶聯免疫吸附測定%免疫組織化學%染色與標記
란포억소상관단백질류%결장염,궤양성%매련면역흡부측정%면역조직화학%염색여표기
Follistatin-related proteins%Colitis,ulcerative%Enzyme-linked immunosorbent assay%Immunohistochemistry%Staining and labeling
目的 检测卵泡抑素样蛋白1(FSTLl)在UC患者外周血和肠黏膜组织中的表达情况,分析其与UC疾病活动性的关系.方法 收集2010年10月至2012年6月UC患者60例,收集2012年4月至10月肠镜和病理检查均未见明显黏膜病变者30例为对照.应用ELISA法检测UC组和对照组患者外周血血浆中FSTL1的表达水平,组间比较行t检验.应用免疫组织化学染色方法检测UC组和对照组患者肠黏膜组织标本中FSTL1的表达情况,组间比较行卡方检验.对UC患者进行UC疾病活动指数(DAI)评分,其与血浆FSTL1水平的相关性行Pearson相关分析.结果 UC组血浆FSTL1表达水平[(14.37±1.80)μg/L]高于对照组[(5.80±0.72) μg/L],差异有统计学意义(t=25.01,P＜0.05).UC患者血浆FSTL1表达水平与UCDAI呈正相关(r=0.814,P＜0.05).UC组肠黏膜组织中FSTL1的阳性表达率[86.7％(52/60)]高于对照组[46.7％(14/30)],差异有统计学意义(x2=52.334,P＜0.05).结论 UC患者FSTL1表达水平上升,并与疾病活动性呈正相关,FSTL1可能在UC发生发展中起一定的作用.
目的 檢測卵泡抑素樣蛋白1(FSTLl)在UC患者外週血和腸黏膜組織中的錶達情況,分析其與UC疾病活動性的關繫.方法 收集2010年10月至2012年6月UC患者60例,收集2012年4月至10月腸鏡和病理檢查均未見明顯黏膜病變者30例為對照.應用ELISA法檢測UC組和對照組患者外週血血漿中FSTL1的錶達水平,組間比較行t檢驗.應用免疫組織化學染色方法檢測UC組和對照組患者腸黏膜組織標本中FSTL1的錶達情況,組間比較行卡方檢驗.對UC患者進行UC疾病活動指數(DAI)評分,其與血漿FSTL1水平的相關性行Pearson相關分析.結果 UC組血漿FSTL1錶達水平[(14.37±1.80)μg/L]高于對照組[(5.80±0.72) μg/L],差異有統計學意義(t=25.01,P＜0.05).UC患者血漿FSTL1錶達水平與UCDAI呈正相關(r=0.814,P＜0.05).UC組腸黏膜組織中FSTL1的暘性錶達率[86.7％(52/60)]高于對照組[46.7％(14/30)],差異有統計學意義(x2=52.334,P＜0.05).結論 UC患者FSTL1錶達水平上升,併與疾病活動性呈正相關,FSTL1可能在UC髮生髮展中起一定的作用.
목적 검측란포억소양단백1(FSTLl)재UC환자외주혈화장점막조직중적표체정황,분석기여UC질병활동성적관계.방법 수집2010년10월지2012년6월UC환자60례,수집2012년4월지10월장경화병리검사균미견명현점막병변자30례위대조.응용ELISA법검측UC조화대조조환자외주혈혈장중FSTL1적표체수평,조간비교행t검험.응용면역조직화학염색방법검측UC조화대조조환자장점막조직표본중FSTL1적표체정황,조간비교행잡방검험.대UC환자진행UC질병활동지수(DAI)평분,기여혈장FSTL1수평적상관성행Pearson상관분석.결과 UC조혈장FSTL1표체수평[(14.37±1.80)μg/L]고우대조조[(5.80±0.72) μg/L],차이유통계학의의(t=25.01,P＜0.05).UC환자혈장FSTL1표체수평여UCDAI정정상관(r=0.814,P＜0.05).UC조장점막조직중FSTL1적양성표체솔[86.7％(52/60)]고우대조조[46.7％(14/30)],차이유통계학의의(x2=52.334,P＜0.05).결론 UC환자FSTL1표체수평상승,병여질병활동성정정상관,FSTL1가능재UC발생발전중기일정적작용.
Objective To examine the expression of follistatin-like protein 1 (FSTL1) in the peripheral blood and intestinal mucosa tissues of ulcerative colitis (UC) patients and analyze the correlation between its expression and the activity of UC.Methods From October 2010 to June 2012,sixty patients with UC were collected.From April 2012 to October 2012,thirty individuals without any obvious mucosa lesion under colonoscope and confirmed by pathological examination were set as control group.The serum expression level of FSTL1 of both UC group and control group were determined by enzyme linked immunosorbent assay (ELISA).t-test was performed for comparison between groups.The expression of FSTL1 in the intestinal mucosa of UC group and control group was detected by immunohistochemistry.Chi-square test was used for comparison between groups.The patients with UC were scored with ulcerative colitis disease activity index (UCDAI).Its correlation with plasma FSTL1 was analyzed by Pearson correlation coefficient.Results The serum expression level of FSTL1 of UC group ((14.37-±-1.80) μg/L) was higher than that of control group ((5.80±0.72) μg/L)and the difference was statistically significant (t=25.01,P＜ 0.05).The serum expression level of FSTL1 of UC group was positively correlated with UCDAI (r=0.814,P＜0.05).The positive expression rate of FSTL1 in the intestinal mucosa tissues of UC group (86.7％,52/60)was higher than that of control group (46.7％,14/30) and the difference was statistically significant (x2 =52.334,P＜0.05).Conclusions The expression of FSTL1 of UC patients increases and is positively correlated with disease activity.FSTL1 may play a role in the development of UC.
