中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2014年
1期
45-48
,共4页
吴迎%胡佳辰%周瑞%夏冰
吳迎%鬍佳辰%週瑞%夏冰
오영%호가신%주서%하빙
炎性肠疾病%蛋白质阵列分析%细胞因子类%印迹法,蛋白质
炎性腸疾病%蛋白質陣列分析%細胞因子類%印跡法,蛋白質
염성장질병%단백질진렬분석%세포인자류%인적법,단백질
Inflammatory bowel diseases%Protein array analysis%Cytokines%Blotting,Western
目的 应用细胞因子抗体芯片技术研究IBD患者肠组织差异表达的蛋白质,探讨筛选出的蛋白在IBD发病机制中可能涉及的作用.方法 收集9例UC、9例CD和9例对照患者的肠组织标本,每组提取总蛋白后,采用Raybiotech L系列人抗体芯片分析各组的差异表达蛋白质谱.另收集9例UC,9例CD和9例对照患者肠组织标本,用Western印迹法对差异较大的蛋白质进行验证.两组间比较采用t检验.结果 与对照组相比,UC组共有263个细胞因子表达发生显著变化,CD组共有414个细胞因子表达发生显著变化.随后采用Western印迹法验证了单纯疱疹病毒进入介导子、白血病抑制因子和血小板因子4在IBD患者肠组织中均高表达,差异均有统计学意义(UC组,t=23.85、9.53、18.88,CD组t=13.54、16.65、13.67,P均＜0.01).结论 采用抗体芯片技术筛选出IBD患者肠组织细胞因子差异表达谱,有利于揭示IBD发病机制及发现新的分子标志物.
目的 應用細胞因子抗體芯片技術研究IBD患者腸組織差異錶達的蛋白質,探討篩選齣的蛋白在IBD髮病機製中可能涉及的作用.方法 收集9例UC、9例CD和9例對照患者的腸組織標本,每組提取總蛋白後,採用Raybiotech L繫列人抗體芯片分析各組的差異錶達蛋白質譜.另收集9例UC,9例CD和9例對照患者腸組織標本,用Western印跡法對差異較大的蛋白質進行驗證.兩組間比較採用t檢驗.結果 與對照組相比,UC組共有263箇細胞因子錶達髮生顯著變化,CD組共有414箇細胞因子錶達髮生顯著變化.隨後採用Western印跡法驗證瞭單純皰疹病毒進入介導子、白血病抑製因子和血小闆因子4在IBD患者腸組織中均高錶達,差異均有統計學意義(UC組,t=23.85、9.53、18.88,CD組t=13.54、16.65、13.67,P均＜0.01).結論 採用抗體芯片技術篩選齣IBD患者腸組織細胞因子差異錶達譜,有利于揭示IBD髮病機製及髮現新的分子標誌物.
목적 응용세포인자항체심편기술연구IBD환자장조직차이표체적단백질,탐토사선출적단백재IBD발병궤제중가능섭급적작용.방법 수집9례UC、9례CD화9례대조환자적장조직표본,매조제취총단백후,채용Raybiotech L계렬인항체심편분석각조적차이표체단백질보.령수집9례UC,9례CD화9례대조환자장조직표본,용Western인적법대차이교대적단백질진행험증.량조간비교채용t검험.결과 여대조조상비,UC조공유263개세포인자표체발생현저변화,CD조공유414개세포인자표체발생현저변화.수후채용Western인적법험증료단순포진병독진입개도자、백혈병억제인자화혈소판인자4재IBD환자장조직중균고표체,차이균유통계학의의(UC조,t=23.85、9.53、18.88,CD조t=13.54、16.65、13.67,P균＜0.01).결론 채용항체심편기술사선출IBD환자장조직세포인자차이표체보,유리우게시IBD발병궤제급발현신적분자표지물.
Objective To investigate differential expressed protein in the intestinal mucosa of patients with inflammatory bowel disease (IBD) with antibody chips,and to explore the possible role of the screened proteins in pathogenesis of IBD.Methods The mucosa tissues of nine patients with ulcerative colitis (UC),nine patients with Crohn's disease (CD) and nine control individuals were collected.After total protein of each group was extracted,the differential expressed protein of each group was analyzed by Raybiotech L-series human cytokine antibody chips.The mucose tissues of other nine patients with UC,nine patients with CD and nine control individuals were collected,and were used to verify the greatly differential expressed proteins by Western blot.The t-test was performed to compare two groups.Results Compared with the control group,there was significantly difference in 263 cytokines of UC group,and 414 cytokines of CD group.And then the higher expressions of herpes virus entry mediator,leukemia inhibitory factor and platelet factor 4 in the mucosa tissues of IBD patients were confirmed by Western blot and the differences were statistically significant (UC:t=23.85,9.53,18.88; CD:t=13.54,16.65,13.67,all P＜0.01).Conclusion The screened differential expressed cytokines in the mucosa tissues of IBD patients by cytokine antibody chips could be helpful to reveal the pathogenesis of IBD and discover new molecular biomarkers.
