CAJ | 학술논문

目的探讨高迁移率蛋白A2(HMGA2)在哈萨克族食管癌中的表达并分析其对上皮间质转化(EMT)的调控作用.方法免疫组织化学SP法检测72例新疆地区哈萨克族食管癌患者癌组织及癌旁组织中HMGA2及EMT相关蛋白Snail、上皮钙黏素的表达,并分析三者与食管癌浸润、转移等临床病理参数的相关性.采用McNemar检验和卡方检验进行率的比较,Spearman法进行相关性分析.结果 HMGA2及Snail在72例食管癌患者癌组织中的表达阳性率分别为83.3％(60/72)、45.8％(33/72),明显高于二者在癌旁组织中的表达(52.8％,38/72,McNemar检验,P＜0.01;29.2％,21/72,McNemar检验,P＜0.05),同时HMGA2与Snail在癌组织中的表达呈正相关(r=0.262,P＜0.05).上皮钙黏素在癌组织中的表达阳性率为40.3％(29/72),明显低于其在癌旁组织中的表达(72.2％,52/72,McNemar检验,P＜0.01).Snail的表达与肿瘤浸润深度相关,肿瘤侵犯至食管外膜及周围组织的患者Snail表达率显著高于仅侵犯至黏膜层及肌层的患者(x2=5.308,P＜0.05),上皮钙黏素的表达与患者的年龄及肿瘤分化程度相关,年龄＜58岁(x2=3.952,P＜0.05)、分化程度较高(x2=8.080,P＜0.05)者上皮钙黏素的表达率较高.结论 HMGA2、Snail在哈萨克族食管癌患者癌组织中显著高表达,上皮钙黏素呈低表达,提示EMT可能参与了哈萨克族患者食管癌的发生、发展.
목적 탐토고천이솔단백A2(HMGA2)재합살극족식관암중적표체병분석기대상피간질전화(EMT)적조공작용.방법 면역조직화학SP법검측72례신강지구합살극족식관암환자암조직급암방조직중HMGA2급EMT상관단백Snail、상피개점소적표체,병분석삼자여식관암침윤、전이등림상병리삼수적상관성.채용McNemar검험화잡방검험진행솔적비교,Spearman법진행상관성분석.결과 HMGA2급Snail재72례식관암환자암조직중적표체양성솔분별위83.3％(60/72)、45.8％(33/72),명현고우이자재암방조직중적표체(52.8％,38/72,McNemar검험,P＜0.01;29.2％,21/72,McNemar검험,P＜0.05),동시HMGA2여Snail재암조직중적표체정정상관(r=0.262,P＜0.05).상피개점소재암조직중적표체양성솔위40.3％(29/72),명현저우기재암방조직중적표체(72.2％,52/72,McNemar검험,P＜0.01).Snail적표체여종류침윤심도상관,종류침범지식관외막급주위조직적환자Snail표체솔현저고우부침범지점막층급기층적환자(x2=5.308,P＜0.05),상피개점소적표체여환자적년령급종류분화정도상관,년령＜58세(x2=3.952,P＜0.05)、분화정도교고(x2=8.080,P＜0.05)자상피개점소적표체솔교고.결론 HMGA2、Snail재합살극족식관암환자암조직중현저고표체,상피개점소정저표체,제시EMT가능삼여료합살극족환자식관암적발생、발전.
Objective To investigate the expression of high mobility group protein A2 (HMGA2) in esophageal squamous cell carcinoma (ESCC) of Kazakh ethnic and its regulation role in epithelial-mesenchymal transition (EMT).Methods The expression of HMGA2 and EMT related protein Snail and E-cadherin in carcinoma tissues and adjacent tissues of 72 Kazakh with ESCC was detected by immunohistochemistry (IHC) SP method,and the correlation among these proteins and invasion as well as metastasis of ESCC was analyzed.McNemar's test and Chi square test were performed for the rate comparison.Spearman method was used for correlation analysis.Results The positive rate of HMGA2 and Snail expression in carcinoma tissues of patients with ESCC was 83.3％ (60/72) and 45.8％ (33/72),which were higher than those of adjacent tissues (52.8 ％,38/72,McNemar's test,P＜0.01 ; 29.2 ％,21/72,McNemar's test,P＜ 0.05).The expression of HMGA2 was positively correlated with the expression of Snail in adjacent tissues (r=0.262,P＜0.05).The positive expression rate of E-cadherin in carcinoma tissues was 40.3 ％ (29/72),which was significantly lower than the expression in carcinoma adjacent tissues (72.2％,52/72,McNemar's test,P＜0.01).The expression of Snail was correlated with depth of tumor invasion.The expression quantity of Snail in patients with tumor invasion to the outer membrane of the esophagus and surrounding tissues was significantly higher than that in patients with tumor invasion to mucous layer and muscular layer (x2 =5.308,P＜0.05).The expression of E-cadherin was correlated with the age of patients and the degree of tumor differentiation.The expression of E-cadherin was higher in patients less than 58 years old (x2 =3.952,P＜0.05) and with high degree of differentiation (x2 =8.080,P＜0.05).Conclusion The low expressions of E-cadherin and the high expressions of HMGA2 and Snail in carcinoma tissues of Kazakh's patients with ESCC indicates that EMT may participate in the genesis and development of ESCC.