中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2014年
4期
256-259
,共4页
刘业六%钱海鑫%张逖%唐晓军%庞利群%刘卫东%李斌
劉業六%錢海鑫%張逖%唐曉軍%龐利群%劉衛東%李斌
류업륙%전해흠%장적%당효군%방리군%류위동%리빈
受体,CCR6%趋化因子CCL20%癌,肝细胞
受體,CCR6%趨化因子CCL20%癌,肝細胞
수체,CCR6%추화인자CCL20%암,간세포
Receptors,CCR6%Chemokine CCL20%Carcinoma,hepatocellular
目的 探讨趋化因子受体6/趋化因子20生物轴在肝癌组织中的表达情况和临床意义.方法 选择2003年3月至2005年12月肝癌患者48例,取其肝癌组织、癌旁组织配对标本各1份.另选取8例肝良性病变患者,取其手术切除的正常肝组织各1份.用实时荧光定量PCR法检测趋化因子受体6和趋化因子20 mRNA相对表达量.用免疫组织化学染色法检测趋化因子受体6和趋化因子20蛋白的表达情况.计量资料组间差异行单因素方差分析,率的比较行卡方检验,采用Spearman法计算相关系数,采用Kaplan-Meier法绘制生存曲线,生存率的比较行Log rank检验.结果 肝癌组织的趋化因子受体6和趋化因子20的mRNA相对表达量(0.99±0.21和0.46±0.11)均高于癌旁组织(0.33±0.09和0.31±0.07)和正常肝组织(0.22±0.06和0.28±0.05),差异均有统计学意义(F=127.43和21.10,P均＜0.05).肝癌组织趋化因子受体6蛋白阳性表达率[54.17％(26/48)]高于癌旁组织[16.67％(8/48)]和正常肝组织(0/8),差异有统计学意义(x2=19.55,P＜0.05).肝癌组织趋化因子20蛋白阳性表达率[50.00％(24/48)]与癌旁组织[33.33％(16/48)]和正常肝组织(2/8)比较差异均无统计学意义(P均＞0.05).肝癌组织中趋化因子受体6蛋白阳性表达率与趋化因子20蛋白阳性表达率之间存在相关性(r=0.42,P＜0.05).趋化因子受体6蛋白阳性表达率与肝癌pTNM分期、有否脉管癌栓、有否肝内转移、有否肺转移相关(x2=5.48、4.02、5.07、5.19,P均＜0.05).趋化因子20蛋白阳性表达率与肝癌肿瘤最大径、pTNM分期相关(x2=4.09、4.00,P均＜0.05).趋化因子受体6蛋白阳性组的无瘤生存率和总体生存率均低于阴性组(x2=4.57和6.57,P均＜0.05).趋化因子20蛋白阳性组的无瘤生存率和总体生存率与阴性组比较差异均无统计学意义(P均＞0.05).结论 趋化因子受体6/趋化因子20生物轴与肝癌的恶性生物学行为和预后相关.
目的 探討趨化因子受體6/趨化因子20生物軸在肝癌組織中的錶達情況和臨床意義.方法 選擇2003年3月至2005年12月肝癌患者48例,取其肝癌組織、癌徬組織配對標本各1份.另選取8例肝良性病變患者,取其手術切除的正常肝組織各1份.用實時熒光定量PCR法檢測趨化因子受體6和趨化因子20 mRNA相對錶達量.用免疫組織化學染色法檢測趨化因子受體6和趨化因子20蛋白的錶達情況.計量資料組間差異行單因素方差分析,率的比較行卡方檢驗,採用Spearman法計算相關繫數,採用Kaplan-Meier法繪製生存麯線,生存率的比較行Log rank檢驗.結果 肝癌組織的趨化因子受體6和趨化因子20的mRNA相對錶達量(0.99±0.21和0.46±0.11)均高于癌徬組織(0.33±0.09和0.31±0.07)和正常肝組織(0.22±0.06和0.28±0.05),差異均有統計學意義(F=127.43和21.10,P均＜0.05).肝癌組織趨化因子受體6蛋白暘性錶達率[54.17％(26/48)]高于癌徬組織[16.67％(8/48)]和正常肝組織(0/8),差異有統計學意義(x2=19.55,P＜0.05).肝癌組織趨化因子20蛋白暘性錶達率[50.00％(24/48)]與癌徬組織[33.33％(16/48)]和正常肝組織(2/8)比較差異均無統計學意義(P均＞0.05).肝癌組織中趨化因子受體6蛋白暘性錶達率與趨化因子20蛋白暘性錶達率之間存在相關性(r=0.42,P＜0.05).趨化因子受體6蛋白暘性錶達率與肝癌pTNM分期、有否脈管癌栓、有否肝內轉移、有否肺轉移相關(x2=5.48、4.02、5.07、5.19,P均＜0.05).趨化因子20蛋白暘性錶達率與肝癌腫瘤最大徑、pTNM分期相關(x2=4.09、4.00,P均＜0.05).趨化因子受體6蛋白暘性組的無瘤生存率和總體生存率均低于陰性組(x2=4.57和6.57,P均＜0.05).趨化因子20蛋白暘性組的無瘤生存率和總體生存率與陰性組比較差異均無統計學意義(P均＞0.05).結論 趨化因子受體6/趨化因子20生物軸與肝癌的噁性生物學行為和預後相關.
목적 탐토추화인자수체6/추화인자20생물축재간암조직중적표체정황화림상의의.방법 선택2003년3월지2005년12월간암환자48례,취기간암조직、암방조직배대표본각1빈.령선취8례간량성병변환자,취기수술절제적정상간조직각1빈.용실시형광정량PCR법검측추화인자수체6화추화인자20 mRNA상대표체량.용면역조직화학염색법검측추화인자수체6화추화인자20단백적표체정황.계량자료조간차이행단인소방차분석,솔적비교행잡방검험,채용Spearman법계산상관계수,채용Kaplan-Meier법회제생존곡선,생존솔적비교행Log rank검험.결과 간암조직적추화인자수체6화추화인자20적mRNA상대표체량(0.99±0.21화0.46±0.11)균고우암방조직(0.33±0.09화0.31±0.07)화정상간조직(0.22±0.06화0.28±0.05),차이균유통계학의의(F=127.43화21.10,P균＜0.05).간암조직추화인자수체6단백양성표체솔[54.17％(26/48)]고우암방조직[16.67％(8/48)]화정상간조직(0/8),차이유통계학의의(x2=19.55,P＜0.05).간암조직추화인자20단백양성표체솔[50.00％(24/48)]여암방조직[33.33％(16/48)]화정상간조직(2/8)비교차이균무통계학의의(P균＞0.05).간암조직중추화인자수체6단백양성표체솔여추화인자20단백양성표체솔지간존재상관성(r=0.42,P＜0.05).추화인자수체6단백양성표체솔여간암pTNM분기、유부맥관암전、유부간내전이、유부폐전이상관(x2=5.48、4.02、5.07、5.19,P균＜0.05).추화인자20단백양성표체솔여간암종류최대경、pTNM분기상관(x2=4.09、4.00,P균＜0.05).추화인자수체6단백양성조적무류생존솔화총체생존솔균저우음성조(x2=4.57화6.57,P균＜0.05).추화인자20단백양성조적무류생존솔화총체생존솔여음성조비교차이균무통계학의의(P균＞0.05).결론 추화인자수체6/추화인자20생물축여간암적악성생물학행위화예후상관.
Objective To explore the expression and clinical significance of chemokine receptor 6 (CCR6)/chemokine ligand 20(CCL20) axis in hepatocellular carcinoma (HCC).Methods From March 2003 to December 2005,48 patients with HCC were selected,and one specimen of HCC tissue and one of corresponding adjacent tissue were taken from every patient.Another eight patients with benign liver lesions were selected,and one specimen of surgical sectioned normal liver tissue of each was taken.The relative expression quantity of CCR6 and CCL20 at mRNA level was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR).And the expression of CCR6 and CCL20 at protein level was determined by immunohistochemisty methods.One-way analysis of variance (ANOVA) was performed for comparison among groups of measurement data.Chi-square test was used for rate comparison.The correlation coefficient was calculated by Spearman's method.Survival curves were plotted by Kaplan-Meier method and the survival rate was compared by Log-rank test.Results The relative expression quantity of CCR6/CCL20 at mRNA level in HCC tissues (0.99±0.21 and 0.46± 0.11) were significantly higher than those of para carcinoma tissues (0.33 ± 0.09 and 0.31 ± 0.07) and normal liver tissues (0.22±0.06 and 0.28±0.05),and the differences were statistically significant (F=127.43 and 21.10,both P＜0.05).The positive percentage of CCR6 protein expression in HCC tissues (54.17％,26/48) was significantly higher than that in para carcinoma tissues (16.67％,8/48) and normal liver tissues (0/8),and the difference was statistically significant (x2 =19.55,P＜0.05).There was no statistically significant difference in the positive percentage of CCL20 protein expression among HCC tissues (50.00％,24/48),paracarcinoma tissues (33.33％,16/48),normal liver tissues (2/8) (all P＜0.05).There was correlation between the positive percentage of CCR6 protein expression and that of CCL20 protein expression in HCC tissues (r=0.42,P＜0.05).The positive percentage of CCR6 protein expression was correlated with the pTNM stage of HCC,vascular tumor thrombosis,intrahepatic metastasis and lung metastasis (x2 =5.48,4.02,5.07 and 5.19,all P＜0.05).The positive percentage of CCL20 expression was significantly correlated to tumor maximum diameter and pTNM stage (x2 =4.09 and 4.00,both P＜0.05).Both the disease-free survival (DFS) rate and overall survival (OS) rate of CCR6-positive group were significantly lower than those of negative group (x2 =4.57 and 6.57,both P＜ 0.05).There were no significant differences in DFS rate and OS rate between CCL20-positive group and negative group (both P＞0.05).Conclusion CCR6/CCL20 axis may be related with the malignant behavior and the prognosis of HCC.
