中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2014年
8期
516-520
,共5页
裴媛媛%杨文秀%孟青%陈琴%李品浩
裴媛媛%楊文秀%孟青%陳琴%李品浩
배원원%양문수%맹청%진금%리품호
淋巴瘤%CARMA1%螺杆菌,幽门
淋巴瘤%CARMA1%螺桿菌,幽門
림파류%CARMA1%라간균,유문
Lymphoma%CARMA1%Helicobacter pylori
目的 探讨含Caspase募集结构域的膜相关鸟苷酸激酶蛋白1(CARMA1)表达与胃肠道MALT淋巴瘤和弥漫大B细胞淋巴瘤(DLBCL)临床病理特征及H.pylori感染的关系.方法 选取1999年1月至2011年3月胃肠道DLBCL患者34例和MALT淋巴瘤患者20例,同时选取21例胃肠淋巴组织反应性增生者为对照.用免疫组织化学染色法检测所有研究对象的Ki-67、CARMA1、细胞毒素相关基因A (CagA)蛋白表达情况.用实时定量PCR方法检测28例DLBCL患者和14例MALT淋巴瘤患者手术所获组织标本中CARMA1 mRNA的相对表达量.用硼酸亚甲蓝染色或半套式PCR方法检测病变部位为胃的25例淋巴瘤患者和10例对照者的H.pylori感染情况.计数资料行卡方检验.计量资料行独立样本t检验.采用COX多因素回归分析法进行生存分析.用Kaplan-Meier法绘制生存曲线并行对数秩和检验.结果 28例DLBCL患者的CARMA1 mRNA相对表达量(3.073±1.846)高于14例MALT淋巴瘤患者,差异有统计学意义(F= 0.975,P<0.05).胃肠道淋巴瘤组的CARMA1蛋白阳性表达率[75.9%(41/54)]高于对照组[47.6%(10/21)],差异有统计学意义(x2=5.568,P<0.05),其中MALT淋巴瘤组(11/20)低于DLBCL组[88.2%(30/34)],差异有统计学意义(x2=5.900,P<0.05).在42例接受手术治疗的胃肠道淋巴瘤患者中,高增殖活性者的CARMA1 mRNA相对表达量(2.885±1.837)高于低增殖活性者,临床晚期病例组CARMA1 mRNA相对表达量(4.416±1.0L0)高于临床早期病例组,差异均有统计学意义(F=3.317和2.972,P均<0.05).在54例胃肠道淋巴瘤患者中,高增殖活性者的CARMA1蛋白阳性表达率[88.6%(31/35)]高于低增殖活性者(10/19),差异有统计学意义(x2=6.847,P<0.05).相对11例未感染H.pylori的胃淋巴瘤患者,14例感染H.pylori的胃淋巴瘤患者CARMA1 mRNA相对表达量和CARMA1蛋白表达阳性率差异均无统计学意义(P均>0.05).CagA阳性和CagA阴性的H.pylori感染胃淋巴瘤患者CARMA1蛋白表达阳性率分别为11/11和2/3.CARMA1蛋白的表达情况与胃肠道淋巴瘤的预后有关(RR= 4.160,P<0.05).在获随访的29例胃肠道淋巴瘤患者和18例DLBCL患者中,CARMA1蛋白阳性表达率≥50%者生存状况较<50%者差,差异均有统计学意义(x2=5.383和4.028,P均<0.05).结论 CARMA1表达可能参与MALT淋巴瘤和DLBCL的发生、发展,并可能是其不良预后的相关因子;这两种淋巴瘤中CARMA1的表达情况与H.pylori感染无关.
目的 探討含Caspase募集結構域的膜相關鳥苷痠激酶蛋白1(CARMA1)錶達與胃腸道MALT淋巴瘤和瀰漫大B細胞淋巴瘤(DLBCL)臨床病理特徵及H.pylori感染的關繫.方法 選取1999年1月至2011年3月胃腸道DLBCL患者34例和MALT淋巴瘤患者20例,同時選取21例胃腸淋巴組織反應性增生者為對照.用免疫組織化學染色法檢測所有研究對象的Ki-67、CARMA1、細胞毒素相關基因A (CagA)蛋白錶達情況.用實時定量PCR方法檢測28例DLBCL患者和14例MALT淋巴瘤患者手術所穫組織標本中CARMA1 mRNA的相對錶達量.用硼痠亞甲藍染色或半套式PCR方法檢測病變部位為胃的25例淋巴瘤患者和10例對照者的H.pylori感染情況.計數資料行卡方檢驗.計量資料行獨立樣本t檢驗.採用COX多因素迴歸分析法進行生存分析.用Kaplan-Meier法繪製生存麯線併行對數秩和檢驗.結果 28例DLBCL患者的CARMA1 mRNA相對錶達量(3.073±1.846)高于14例MALT淋巴瘤患者,差異有統計學意義(F= 0.975,P<0.05).胃腸道淋巴瘤組的CARMA1蛋白暘性錶達率[75.9%(41/54)]高于對照組[47.6%(10/21)],差異有統計學意義(x2=5.568,P<0.05),其中MALT淋巴瘤組(11/20)低于DLBCL組[88.2%(30/34)],差異有統計學意義(x2=5.900,P<0.05).在42例接受手術治療的胃腸道淋巴瘤患者中,高增殖活性者的CARMA1 mRNA相對錶達量(2.885±1.837)高于低增殖活性者,臨床晚期病例組CARMA1 mRNA相對錶達量(4.416±1.0L0)高于臨床早期病例組,差異均有統計學意義(F=3.317和2.972,P均<0.05).在54例胃腸道淋巴瘤患者中,高增殖活性者的CARMA1蛋白暘性錶達率[88.6%(31/35)]高于低增殖活性者(10/19),差異有統計學意義(x2=6.847,P<0.05).相對11例未感染H.pylori的胃淋巴瘤患者,14例感染H.pylori的胃淋巴瘤患者CARMA1 mRNA相對錶達量和CARMA1蛋白錶達暘性率差異均無統計學意義(P均>0.05).CagA暘性和CagA陰性的H.pylori感染胃淋巴瘤患者CARMA1蛋白錶達暘性率分彆為11/11和2/3.CARMA1蛋白的錶達情況與胃腸道淋巴瘤的預後有關(RR= 4.160,P<0.05).在穫隨訪的29例胃腸道淋巴瘤患者和18例DLBCL患者中,CARMA1蛋白暘性錶達率≥50%者生存狀況較<50%者差,差異均有統計學意義(x2=5.383和4.028,P均<0.05).結論 CARMA1錶達可能參與MALT淋巴瘤和DLBCL的髮生、髮展,併可能是其不良預後的相關因子;這兩種淋巴瘤中CARMA1的錶達情況與H.pylori感染無關.
목적 탐토함Caspase모집결구역적막상관조감산격매단백1(CARMA1)표체여위장도MALT림파류화미만대B세포림파류(DLBCL)림상병리특정급H.pylori감염적관계.방법 선취1999년1월지2011년3월위장도DLBCL환자34례화MALT림파류환자20례,동시선취21례위장림파조직반응성증생자위대조.용면역조직화학염색법검측소유연구대상적Ki-67、CARMA1、세포독소상관기인A (CagA)단백표체정황.용실시정량PCR방법검측28례DLBCL환자화14례MALT림파류환자수술소획조직표본중CARMA1 mRNA적상대표체량.용붕산아갑람염색혹반투식PCR방법검측병변부위위위적25례림파류환자화10례대조자적H.pylori감염정황.계수자료행잡방검험.계량자료행독립양본t검험.채용COX다인소회귀분석법진행생존분석.용Kaplan-Meier법회제생존곡선병행대수질화검험.결과 28례DLBCL환자적CARMA1 mRNA상대표체량(3.073±1.846)고우14례MALT림파류환자,차이유통계학의의(F= 0.975,P<0.05).위장도림파류조적CARMA1단백양성표체솔[75.9%(41/54)]고우대조조[47.6%(10/21)],차이유통계학의의(x2=5.568,P<0.05),기중MALT림파류조(11/20)저우DLBCL조[88.2%(30/34)],차이유통계학의의(x2=5.900,P<0.05).재42례접수수술치료적위장도림파류환자중,고증식활성자적CARMA1 mRNA상대표체량(2.885±1.837)고우저증식활성자,림상만기병례조CARMA1 mRNA상대표체량(4.416±1.0L0)고우림상조기병례조,차이균유통계학의의(F=3.317화2.972,P균<0.05).재54례위장도림파류환자중,고증식활성자적CARMA1단백양성표체솔[88.6%(31/35)]고우저증식활성자(10/19),차이유통계학의의(x2=6.847,P<0.05).상대11례미감염H.pylori적위림파류환자,14례감염H.pylori적위림파류환자CARMA1 mRNA상대표체량화CARMA1단백표체양성솔차이균무통계학의의(P균>0.05).CagA양성화CagA음성적H.pylori감염위림파류환자CARMA1단백표체양성솔분별위11/11화2/3.CARMA1단백적표체정황여위장도림파류적예후유관(RR= 4.160,P<0.05).재획수방적29례위장도림파류환자화18례DLBCL환자중,CARMA1단백양성표체솔≥50%자생존상황교<50%자차,차이균유통계학의의(x2=5.383화4.028,P균<0.05).결론 CARMA1표체가능삼여MALT림파류화DLBCL적발생、발전,병가능시기불량예후적상관인자;저량충림파류중CARMA1적표체정황여H.pylori감염무관.
Objective To investigate the relation between the expression of Caspase recruitment domain-containing membrane-associated guanylate kinase protein 1 (CARMA1),clinicopathological features of gastrointestinal mucosa-associated lymphoid tissue (MALT) lymphoma,diffuse large B cell lymphoma (DLBCL),and Helicobacter pylori (H.pylori) infection.Methods From January 1999 to March 2011,34 patients with DLBCL and 20 patients with MALT lymphoma were selected,and at same period 21 cases with reactive hyperplasia of gastrointestinal lymphoid tissue were enrolled in as control.The expression of CARMA1,Ki-67 and cytotoxin-associated gene A (CagA) at protein level were examined by immunohistochemistry.The relative expression quantity of CARMA1 mRNA was detected by real-time polymerase chain reaction (PCR).The condition of H.pylori infection in 25 gastric lymphoma and 10 controls was detected by methylene boric acid and blue staining or semi-nested PCR.Chi-square test was used for counting data analysis,t test for measurement data.Multivariate COX regression analysis was implemented for survival analysis.Survival curve was drawn by Kaplan-Meier method and analyzed by Log-rank test.Results The relative expression quantity of CARMA1 mRNA of 28 patients with DLBLC (3.073±1.846) was higher than that of 14 patients with MALT lymphoma,and the difference was statistically significant (F 0.975,P< 0.05).The positive rate of CARMA1 expression at protein level of gastrointestinal lymphoma group (75.9 %,41/54) was higher than that of control group (47.6%,10/21),and the difference was statistically significant (x2 =5.568,P<0.05),and the positive rate of CARMA1 expression of MALT lymphoma group (11/20) was lower than that of DLBCL group (88.2%,30/34),and the difference was statistically significant (x2 =5.900,P<0.05).Among 42 patients with gastrointestinal lymphoma who received surgery,the relative expression quantity of CARMA1 mRNA in cases with high proliferation (2.885±1.837) was higher than that in cases with low proliferation.The expression of CARMA1 mRNA in the cases at advanced stage of the disease (4.416± 1.010) was higher than that in cases at early stage,and the difference was statistically significant (F=3.317 and 2.972,both P<0.05).Among 54 patients with gastrointestinal lymphoma,the positive rate of CARMA1 expression at protein level of patients with high proliferation (88.6%,31/35) was higher that that of patients with low proliferation (10/19),and the difference was statistically significant (x22 = 6.847,P<0.05).There were no significant differences in relative expression quantity of CARMA1 mRNA and the positive rate of CARMA1 expression at protein level between 11 gastric lymphoma patients without H.pylori infection and 14 gastric lymphoma patients with H.pylori infection (both P>0.05).The positive rate of CARMA1 expression at protein level of CagA positive and CagA negative H.pylori infected gastric lymphoma patients was 11/11 and 2/3.The expression of CARMA1 at protein level was correlated with the prognosis of gastrointestinal lymphoma (RR=4.160,P<0.05).In the 29 cases of patients with gastrointestinal MALT lymphoma and 18 cases of patients with DLBCL who were followed up,the survival situation of gastrointestinal lymphoma patients with CARMA1 positive expression rate over 50% was worse than that of patients with the rate less than 50%,and the difference was statistically significant (x2=5.383 and 4.028,both P<0.05).Conclusions CARMA1 might be involved in the pathogenesis and progression of MALT and DLBCL; and it might be a related factor of poor prognosis.There was no correlation between the expression of CARMA1 and H.pylori infection in these two lymphomas.