CAJ | 학술논문

目的研究白藜芦醇对大鼠心肌梗死后室性心律失常、心室重构和长期存活率的影响.方法将SD大鼠随机分成假手术组、心肌梗死组、白藜芦醇治疗组(治疗组).心肌梗死组和治疗组开胸结扎冠状动脉左前降支,假手术组不结扎.植入性射频发射器记录24 h心电图,并分析心肌梗死后24 h内室性心律失常发病率;侵入性在体电生理检测评价室性心律失常的诱发率;全细胞膜片钳技术检测白藜芦醇对单个心室肌细胞的电生理作用;免疫荧光染色观察心肌细胞的结构改变.结果与心肌梗死组相比,治疗组明显抑制了心肌梗死导致的室性心动过速和心室颤动发病率,治疗组室性心动过速的诱发率低于心肌梗死组,差异有统计学意义(46%vs 81%,P<0.01).14周后,治疗组心肌梗死面积和死亡率较心肌梗死组分别下降了20%和33%.膜片钳记录显示白藜芦醇抑制了L型钙电流.免疫荧光染色发现治疗组心肌细胞横断面积小于心肌梗死组.结论白藜芦醇可以通过抑制大鼠心肌L型钙电流发挥抗心律失常作用,并抑制了左心室重构,提高了大鼠心肌梗死后的存活率.
목적 연구백려호순대대서심기경사후실성심률실상、심실중구화장기존활솔적영향.방법 장SD대서수궤분성가수술조、심기경사조、백려호순치료조(치료조).심기경사조화치료조개흉결찰관상동맥좌전강지,가수술조불결찰.식입성사빈발사기기록24 h심전도,병분석심기경사후24 h내실성심률실상발병솔;침입성재체전생리검측평개실성심률실상적유발솔;전세포막편겸기술검측백려호순대단개심실기세포적전생리작용;면역형광염색관찰심기세포적결구개변.결과 여심기경사조상비,치료조명현억제료심기경사도치적실성심동과속화심실전동발병솔,치료조실성심동과속적유발솔저우심기경사조,차이유통계학의의(46%vs 81%,P<0.01).14주후,치료조심기경사면적화사망솔교심기경사조분별하강료20%화33%.막편겸기록현시백려호순억제료L형개전류.면역형광염색발현치료조심기세포횡단면적소우심기경사조.결론 백려호순가이통과억제대서심기L형개전류발휘항심률실상작용,병억제료좌심실중구,제고료대서심기경사후적존활솔.
Objective The effects of resveratrol treatment on ventricular arthythmias,survival,and late cardiac remodeling were evaluated in rats with myocardial infarction(MI).Methods Three groups of rats (sham-operated,MI,and MI pre-treated with resveratrel)were treated in an in vivo MI model.The electrocardiogram(ECG)signals were momtored and recorded for 24 hours using an implanted telemetry transmitter.The incidence of ventricular arrhythmias during the first 24-hour after MI was also evaluated.Finally,the electrophysiological effects of resveratrol on isolated rat ventricuhr myocytes were examined by using the whole cell patch clamp method.Results Administration of resveratrol significantly suppressed the MI-induced ventricular tachycardia and ventricularfibrillation(0.4±0.2 in Resv group vs.7.1±2.2 in MI group episodes per hour per rat,P<0.01).Data also showed that the incidence of inducible ventricular tachycardia Wag lower in the Resv group than the MI greup(46%vs 81%,P<0.01).The infarct size and mortality in the Resv group at 14 weeks were reduced by 20%and 33%,respectively,compared with the MI groups.Results from patch clamp recording revesled that resveratrol inhibited L-type calcium current(ICa-L).Conclusion These results suggested that the emerging anti-arrbythmic character induced by resveratrol treatment in rat hearts could be mainly accounted for by inhibition of ICa-L.Administration of resveratrol also improved the long-term survival by suppressing left ventricular remodeling.