CAJ | 학술논문

目的探讨新疆哈萨克族高血压病患者外周血T淋巴细胞电压依赖性钾通道(Kv1.3钾通道)及钙激活钾通道(IKCa1钾通道)的电流变化.方法读取随机数字表法随机选取20例首次就诊且未经药物治疗的新疆哈萨克族高血压病患者作为高血压组,20例哈萨克族健康体检者作为对照组.采用免疫磁珠法分离两组患者外周血T淋巴细胞,运用全细胞膜片钳电流记录的方法,记录T淋巴细胞膜上Kv1.3和IKCa1钾通道电流特性.结果高血压组患者T淋巴细胞Kv1.3钾通道峰值电流密度明显高于对照组[(280±74) pA/pF(n =39)比(179 ±51) pA/pF(n=38),P＜0.01],Kv1.3钾通道膜电容与对照组比较差异则无统计学意义[(2.7±0.7)pF比(2.9±0.6) pF,P＞0.05].高血压组患者T淋巴细胞IKCa1钾通道峰值电流密度明显高于对照组[(198 ±44) pA/pF(n =28)比(124 ±43) pA/pF(n=26),P＜0.01],IKCa1钾通道膜电容与对照组比较差异则无统计学意义[(2.8±0.8)pF比(2.4±0.8) pF,P＞0.05].结论新疆哈萨克族高血压病患者外周血T淋巴细胞Kv1.3及IKCa1钾通道电流密度增加,提示Kv1.3和IKCa1钾通道在新疆哈萨克族高血压病患者的T淋巴细胞的激活过程中起着重要作用.
목적 탐토신강합살극족고혈압병환자외주혈T림파세포전압의뢰성갑통도(Kv1.3갑통도)급개격활갑통도(IKCa1갑통도)적전류변화.방법 독취수궤수자표법수궤선취20례수차취진차미경약물치료적신강합살극족고혈압병환자작위고혈압조,20례합살극족건강체검자작위대조조.채용면역자주법분리량조환자외주혈T림파세포,운용전세포막편겸전류기록적방법,기록T림파세포막상Kv1.3화IKCa1갑통도전류특성.결과 고혈압조환자T림파세포Kv1.3갑통도봉치전류밀도명현고우대조조[(280±74) pA/pF(n =39)비(179 ±51) pA/pF(n=38),P＜0.01],Kv1.3갑통도막전용여대조조비교차이칙무통계학의의[(2.7±0.7)pF비(2.9±0.6) pF,P＞0.05].고혈압조환자T림파세포IKCa1갑통도봉치전류밀도명현고우대조조[(198 ±44) pA/pF(n =28)비(124 ±43) pA/pF(n=26),P＜0.01],IKCa1갑통도막전용여대조조비교차이칙무통계학의의[(2.8±0.8)pF비(2.4±0.8) pF,P＞0.05].결론 신강합살극족고혈압병환자외주혈T림파세포Kv1.3급IKCa1갑통도전류밀도증가,제시Kv1.3화IKCa1갑통도재신강합살극족고혈압병환자적T림파세포적격활과정중기착중요작용.
Objective To observe the current changes of voltage-dependent potassium channel (Kv1.3 potassium channel) and calcium-activated potassium channel (IKCa1 potassium channel) in peripheral blood T-lymphocyte derived from hypertensive patients of Xinjiang Kazakh.Methods Twenty randomly selected untreated Kazakh hypertensive patients and 20 Kazakh healthy subjects from Xinjiang were included in this study.T-lymphocytes were isolated from peripheral blood with magnetic cell sorting,the whole-cell currents of Kv1.3 and IKCa1 potassium channels were recorded with patch-clamp technique.Results (1) The current density of Kv1.3 potassium channel was significantly higher in the hypertensive group [(280 ±74) pA/pF (n =39)] than that in the control group [(179 ±51) pA/pF (n =38),P ＜ 0.01],while the membrane capacitance was similar between the two groups.(2) The current density of IKCa1 potassium channel was also significantly higher in the hypertensive group [(198 ± 44) pA/pF (n =28)] than that in the control group [(124 ± 43) pA/pF (n =26),P ＜ 0.01],while the membrane capacitance was also similar between the two groups.Conclusions The T-lymphocytes Kv1.3 potassium channel and IKCa1 potassium channel current densities are higher in hypertensive patients in Xinjiang Kazakh suggesting a potential role of Kv1.3 and IKCa1 potassium channels activation in the pathophysiology of hypertension.